LOCUS       Exported               10280 bp ds-DNA     circular SYN 02-JAN-2020
DEFINITION  Tobacco rattle virus RNA2-based VIGS vector pTRV2, complete 
            sequence.
ACCESSION   AF406991
VERSION     .
KEYWORDS    pNC-TRV2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP.
  TITLE     Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for 
            N-mediated resistance to tobacco mosaic virus
  JOURNAL   Plant J. 30 (4), 415-429 (2002)
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   Liu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2001) MCDB, Yale University, 165 Prospect Street, 
            New Haven, CT 06520, USA
REFERENCE   3  (bases 1 to 10280)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Tobacco rattle virus RNA2-based VIGS vector 
                     pTRV2"
                     /mol_type="other DNA"
                     /note="Tobacco rattle virus RNA2-based T-DNA vector; 
                     synonym: pYL156"
                     /db_xref="taxon:188057"
     misc_feature    1..1639
                     /label=TRV2
                     /note="TRV strain ppk20 RNA2 5'-sequence"
     misc_feature    1648..2322
                     /label=NC Frame
     misc_feature    2326..2720
                     /label=TRV2
                     /note="TRV strain ppk20 RNA2 3'-sequence"
     terminator      2833..3085
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3107..3131
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4431..5060
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5887..6561
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="SVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFC
                     RRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGR
                     PEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLF
                     KTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASA
                     GS"
     rep_origin      complement(7491..8079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8166..8960)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9385..9409
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      9512..10280
                     /regulatory_class="promoter"
                     /note="Unknown feature type:regulatory  CaMV 35S promoter 
                     from pCASS2"
ORIGIN
        1 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat
       61 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc
      121 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac
      181 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt
      241 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac
      301 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg
      361 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt
      421 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg
      481 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg
      541 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg
      601 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga
      661 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac
      721 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg
      781 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag
      841 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga
      901 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg
      961 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt
     1021 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact
     1081 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc
     1141 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg
     1201 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat
     1261 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc
     1321 gttggtagca tttgagtttc gcaatgcacg aattacttag gaagtggctt gacgacacta
     1381 atgtgttatt gttagataat ggtttggtgg tcaaggtacg tagtagagtc ccacatattc
     1441 gcacgtatga agtaattgga aagttgtcag tttttgataa ttcactggga gatgatacgc
     1501 tgtttgaggg aaaagtagag aacgtatttg tttttatgtt caggcggttc ttgtgtgtca
     1561 acaaagatgg acattgttac tcaaggaagc acgatgagct ttattattac ggacgagtgg
     1621 acttagattc tgtgagtaag gttaccgagt ggtctctgtc cagtcctggc ctcgtcggcc
     1681 attctcgact aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac
     1741 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     1801 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     1861 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1921 ggaagctaaa cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac
     1981 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     2041 gcctgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     2101 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     2161 tatggccagt gtgccggtat ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2221 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2281 tatacacagg gccagtctgg ccacttgtgg tctgctgaga ccggcatgtc ccgaagacat
     2341 taaactacgg ttctttaagt agatccgtgt ctgaagtttt aggttcaatt taaacctacg
     2401 agattgacat tctcgactga tcttgattga tcggtaagtc ttttgtaatt taattttctt
     2461 tttgatttta ttttaaattg ttatctgttt ctgtgtatag actgtttgag atcggcgttt
     2521 ggccgactca ttgtcttacc ataggggaac ggactttgtt tgtgttgtta ttttatttgt
     2581 attttattaa aattctcaac gatctgaaaa agcctcgcgg ctaagagatt gttggggggt
     2641 gagtaagtac ttttaaagtg atgatggtta caaaggcaaa aggggtaaaa cccctcgcct
     2701 acgtaagcgt tattacgccc gtctgtactt atatcagtac actgacgagt ccctaaagga
     2761 cgaaacggga gaacgctagc caccaccacc accaccacgt gtgaattaca ggtgaccagc
     2821 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2881 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     2941 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     3001 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     3061 gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt
     3121 ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg
     3181 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     3241 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     3301 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     3361 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     3421 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     3481 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     3541 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     3601 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     3661 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     3721 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     3781 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     3841 gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc ctaccctcac
     3901 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     3961 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     4021 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     4081 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     4141 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     4201 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     4261 gccggtttgt ctgatgccaa gctggcggcc tggccggcga gcttggccgc tgaagaaacc
     4321 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     4381 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     4441 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     4501 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     4561 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     4621 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     4681 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     4741 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     4801 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     4861 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     4921 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     4981 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     5041 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     5101 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     5161 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     5221 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     5281 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     5341 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     5401 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     5461 ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa
     5521 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag
     5581 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     5641 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     5701 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     5761 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     5821 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggcgggc
     5881 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     5941 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     6001 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     6061 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     6121 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     6181 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     6241 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     6301 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     6361 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     6421 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     6481 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     6541 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     6601 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     6661 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     6721 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     6781 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     6841 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     6901 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     6961 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     7021 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7081 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7141 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7201 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7261 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7921 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     8161 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     8221 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     8281 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     8341 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     8401 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     8461 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     8521 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     8581 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     8641 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     8701 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     8761 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     8821 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     8881 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     8941 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     9001 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     9061 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     9121 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     9181 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     9241 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     9301 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     9361 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     9421 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     9481 tcctaggcca ccatgttggg cccggcgcgc caagcttgca tgcctgcagg tcaacatggt
     9541 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
     9601 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     9661 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
     9721 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
     9781 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     9841 gcaagtggat tgatgtgatg gtcaacatgg tggagcacga cactctcgtc tactccaaga
     9901 atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa caaagggtaa
     9961 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    10021 tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
    10081 aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg
    10141 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
    10201 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    10261 gttcatttca tttggagagg
//
