LOCUS       Exported               11186 bp ds-DNA     circular SYN 12-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             545..1261
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1263..1937
                     /label=NC Frame
     terminator      1964..2216
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2238..2262
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3562..4191
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4625..5692
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5758..5952
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6296..6436
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6622..7210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7297..8091)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8516..8540
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8618..8792
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8832..9857)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9925..10602)
                     /label=CaMV 35S promoter
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        10829..10859
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10867..10883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             10903..11136
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      601 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      661 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      721 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      781 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      841 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      901 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      961 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1021 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1081 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1141 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1201 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1261 gcagtggtct ctgtccagtc ctggcctcgt cggccattct cgactaagtt ggcagcatca
     1321 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
     1381 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     1441 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1501 gtattttttg agttatcgag attttcagga gctaaggaag ctaaactttt gctgacgaga
     1561 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     1621 ctgtttgtgg atgtacagag tgatattatt gacacgcctg ggcgacggat ggtgatcccc
     1681 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     1741 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtatccgtt
     1801 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     1861 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagggccag tctggccact
     1921 tgtggtctgc tgagaccgtg aggtgaccag ctcgaatttc cccgatcgtt caaacatttg
     1981 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     2041 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     2101 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     2161 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     2221 attaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     2281 tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     2341 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     2401 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     2461 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     2521 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     2581 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     2641 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     2701 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     2761 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     2821 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     2881 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     2941 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag
     3001 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     3061 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     3121 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     3181 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     3241 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     3301 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     3361 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     3421 ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     3481 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     3541 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     3601 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     3661 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     3721 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     3781 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     3841 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     3901 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     3961 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     4021 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     4081 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     4141 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     4201 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     4261 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     4321 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     4381 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     4441 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     4501 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     4561 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     4621 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     4681 ggagaagttg aaggcggcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     4741 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     4801 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     4861 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     4921 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     4981 gcacgtagag gtttccgcag ggccggcggg catggccagt gtgtgggatt acgacctggt
     5041 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     5101 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     5161 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     5221 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     5281 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     5341 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     5401 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     5461 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     5521 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     5581 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     5641 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     5701 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
     5761 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     5821 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     5881 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     5941 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     6001 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     6061 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     6121 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     6181 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6241 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     6301 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     6361 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6421 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7261 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
     7321 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     7381 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     7441 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     7501 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     7561 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     7621 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     7681 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     7741 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     7801 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     7861 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     7921 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     7981 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     8041 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     8101 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     8161 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     8221 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     8281 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     8341 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     8401 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     8461 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     8521 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     8581 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
     8641 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
     8701 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
     8761 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt
     8821 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     8881 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     8941 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     9001 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     9061 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     9121 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     9181 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     9241 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     9301 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     9361 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     9421 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     9481 tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg
     9541 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     9601 ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     9661 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     9721 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     9781 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     9841 agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag
     9901 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
     9961 tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
    10021 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    10081 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    10141 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    10201 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    10261 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    10321 tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg
    10381 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    10441 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    10501 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    10561 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    10621 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca
    10681 agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10741 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10801 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10861 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat
    10921 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg
    10981 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11041 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    11101 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
    11161 gtcgtttccc gccttcagtt tagctt
//
