LOCUS       Exported                5465 bp ds-DNA     circular SYN 04-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             545..1219
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    1220..1895
                     /label=NC Frame
     terminator      1922..2174
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2638..2662
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2753..3341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3512..4327)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4618..5053
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5188..5210
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
      601 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      661 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
      721 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      781 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      841 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      901 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      961 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
     1021 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     1081 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
     1141 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     1201 ccgccactcc accggcgccc agtggtctct gtccagtcct ggcctcgtcg gccattctcg
     1261 actaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat
     1321 cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac
     1381 ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     1441 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1501 aaacttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
     1561 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcctggg
     1621 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1681 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1741 agtgtgccgg tatccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1801 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     1861 agggccagtc tggccacttg tggtctgctg agaccgtgag gtgaccagct cgaatttccc
     1921 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     1981 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     2041 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     2101 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     2161 tatgttacta gatcgggcct gcagcccggg ggatccacta gttctagagc ggccgccacc
     2221 gcggtggagc tccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
     2281 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2341 agccggaagh cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg aaggccttga
     2641 caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag
     2701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3241 gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3481 aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc atcaaatgaa
     3541 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     3601 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     3661 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     3721 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     3781 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     3841 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     3901 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3961 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     4021 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     4081 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacaacat
     4141 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     4201 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     4261 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
     4321 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     4381 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     4441 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
     4501 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
     4561 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcgatcccca
     4621 tccaacagcc cgccgtcgag cgggcttttt tatccccgga agcctgtgga tagagggtag
     4681 ttatccacgt gaaaccgcta atgccccgca aagccttgat tcacggggct ttccggcccg
     4741 ctccaaaaac tatccacgtg aaatcgctaa tcagggtacg tgaaatcgct aatcggagta
     4801 cgtgaaatcg ctaataaggt cacgtgaaat cgctaatcaa aaaggcacgt gagaacgcta
     4861 atagcccttt cagatcaaca gcttgcaaac acccctcgct ccggcaagta gttacagcaa
     4921 gtagtatgtt caattagctt ttcaattatg aatatatata tcaattattg gtcgcccttg
     4981 gcttgtggac aatgcgctac gcgcaccggc tccgcccgtg gacaaccgca agcggttgcc
     5041 caccgtcgag cgccagcgcc tttgcccaca acccggcggc cggccgcaac agatcgtttt
     5101 ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc gggcttctgg
     5161 atttccgatc cccggaatta gagatcttgg caggatatat tgtggtgtaa cgttaacatt
     5221 aacgtttaca atttcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5281 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5341 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     5401 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat
     5461 cgata
//
