LOCUS       Exported                5464 bp ds-DNA     circular SYN 04-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    544..1219
                     /label=NC Frame
     CDS             1221..1895
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1921..2173
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2637..2661
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2752..3340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3511..4326)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4617..5052
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5187..5209
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacagtggt ctctgtccag tcctggcctc gtcggccatt ctcgactaag ttggcagcat
      601 cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa
      661 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
      721 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      781 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaactt ttgctgacga
      841 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
      901 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc tgggcgacgg atggtgatcc
      961 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     1021 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtatccg
     1081 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     1141 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagggcc agtctggcca
     1201 cttgtggtct gctgagaccg atggcctcct ccgaggacgt catcaaggag ttcatgcgct
     1261 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1321 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     1381 tgcccttcgc ctgggacatc ctgtcccctc agttccagta cggctccaag gcctacgtga
     1441 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1501 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1561 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1621 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcggatg taccccgagg
     1681 acggcgccct gaagggcgag atcaagatga ggctgaagct gaaggacggc ggccactacg
     1741 acgccgaggt caagaccacc tacatggcca agaagcccgt gcagctgccc ggcgcctaca
     1801 agaccgacat caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1861 acgagcgcgc cgagggccgc cactccaccg gcgcctgagg tgaccagctc gaatttcccc
     1921 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     1981 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     2041 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     2101 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     2161 atgttactag atcgggcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     2221 cggtggagct ccagcttttg ttccctttag tgagggttaa ttccgagctt ggcgtaatca
     2281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2341 gccggaaghc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2401 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatga aggccttgac
     2641 aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc
     2701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag
     3241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3481 aagtatatat gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca tcaaatgaaa
     3541 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     3601 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     3661 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     3721 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     3781 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     3841 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     3901 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     3961 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     4021 tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     4081 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacaacatt
     4141 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     4201 tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     4261 atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg
     4321 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     4381 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg
     4441 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     4501 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     4561 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat
     4621 ccaacagccc gccgtcgagc gggctttttt atccccggaa gcctgtggat agagggtagt
     4681 tatccacgtg aaaccgctaa tgccccgcaa agccttgatt cacggggctt tccggcccgc
     4741 tccaaaaact atccacgtga aatcgctaat cagggtacgt gaaatcgcta atcggagtac
     4801 gtgaaatcgc taataaggtc acgtgaaatc gctaatcaaa aaggcacgtg agaacgctaa
     4861 tagccctttc agatcaacag cttgcaaaca cccctcgctc cggcaagtag ttacagcaag
     4921 tagtatgttc aattagcttt tcaattatga atatatatat caattattgg tcgcccttgg
     4981 cttgtggaca atgcgctacg cgcaccggct ccgcccgtgg acaaccgcaa gcggttgccc
     5041 accgtcgagc gccagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
     5101 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
     5161 tttccgatcc ccggaattag agatcttggc aggatatatt gtggtgtaac gttaacatta
     5221 acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5281 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5341 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     5401 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
     5461 gata
//
