LOCUS       Exported                5498 bp ds-DNA     circular SYN 04-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             545..1252
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1253..1928
                     /label=NC Frame
     terminator      1955..2207
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2671..2695
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2786..3374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3545..4360)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4651..5086
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5221..5243
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      601 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      661 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      721 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      781 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      841 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      901 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
      961 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     1021 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     1081 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     1141 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     1201 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agcagtggtc
     1261 tctgtccagt cctggcctcg tcggccattc tcgactaagt tggcagcatc acccgacgca
     1321 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt
     1381 gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg
     1441 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
     1501 gagttatcga gattttcagg agctaaggaa gctaaacttt tgctgacgag aacagggact
     1561 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     1621 gatgtacaga gtgatattat tgacacgcct gggcgacgga tggtgatccc cctggccagt
     1681 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     1741 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtatccgt tatcggggaa
     1801 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     1861 tggggaatat aaatgtcagg ctcccttata cacagggcca gtctggccac ttgtggtctg
     1921 ctgagaccgt gaggtgacca gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     1981 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     2041 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     2101 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     2161 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg cctgcagccc
     2221 gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
     2281 ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2341 ttgttatccg ctcacaattc cacacaacat acgagccgga aghcataaag tgtaaagcct
     2401 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2461 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2521 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2581 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2641 gggataacgc aggaaagaac atgaaggcct tgacaggata tattggcggg taaactaagt
     2701 cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc
     2761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3241 tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct tgatccggca
     3301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa cattggtcta
     3541 gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     3601 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     3661 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     3721 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     3781 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     3841 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     3901 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     3961 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     4021 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg
     4081 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     4141 agtttagtct gaccatctca tctgtaacaa cattggcaac gctacctttg ccatgtttca
     4201 gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc
     4261 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     4321 gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     4381 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     4441 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     4501 aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca
     4561 aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct
     4621 ggctggatga tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggctt
     4681 ttttatcccc ggaagcctgt ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc
     4741 gcaaagcctt gattcacggg gctttccggc ccgctccaaa aactatccac gtgaaatcgc
     4801 taatcagggt acgtgaaatc gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga
     4861 aatcgctaat caaaaaggca cgtgagaacg ctaatagccc tttcagatca acagcttgca
     4921 aacacccctc gctccggcaa gtagttacag caagtagtat gttcaattag cttttcaatt
     4981 atgaatatat atatcaatta ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc
     5041 ggctccgccc gtggacaacc gcaagcggtt gcccaccgtc gagcgccagc gcctttgccc
     5101 acaacccggc ggccggccgc aacagatcgt tttataaatt tttttttttg aaaaagaaaa
     5161 agcccgaaag gcggcaacct ctcgggcttc tggatttccg atccccggaa ttagagatct
     5221 tggcaggata tattgtggtg taacgttaac attaacgttt acaatttcgc gccattcgcc
     5281 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5341 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5401 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     5461 gggtaccggg ccccccctcg aggtcgacgg tatcgata
//
