LOCUS       Exported                5622 bp ds-DNA     circular SYN 13-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
COMMENT     http://www.informaxinc.com/.
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(30..49)
                     /label=T7
                     /note="Geneious name: T7"
     rep_origin      213..668
                     /label=f1_ORI
                     /note="Geneious name: f1_ORI"
     misc_feature    complement(851..1711)
                     /label=AmpR
                     /note="Geneious name: AmpR"
     misc_feature    2956..3983
                     /label=p35S
                     /note="Geneious name: p35S;Geneious type: promoter 
                     uukaryotic"
     primer_bind     3842..3861
                     /label=35S-F
                     /note="Geneious name: 35S-F"
     CDS             4000..4716
                     /label=Egfp
                     /note="Geneious name: Egfp"
     misc_feature    4718..5392
                     /label=NC Frame
     terminator      complement(5397..5622)
                     /label=T35S
                     /note="Geneious name: T35S"
ORIGIN
        1 ggcaggcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg
       61 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      121 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      181 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
      241 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      301 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      361 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      421 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      481 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      541 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
      601 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
      661 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
      721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1081 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1381 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2521 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2581 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2641 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2701 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2761 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2821 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2881 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
     2941 ctctcccata tggtcgacta gagccaagct gatctccttt gccccggaga tcaccatgga
     3001 cgactttctc tatctctacg atctaggaag aaagttcgac ggagaaggtg acgataccat
     3061 gttcaccacc gataatgaga agattagcct cttcaatttc agaaagaatg ctgacccaca
     3121 gatggttaga gaggcctacg cggcaggtct catcaagacg atctacccga gtaataatct
     3181 ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
     3241 ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg
     3301 gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaagaa
     3361 agtagttcct actgaatcaa aggccatgga gtcaaaaatt cagatcgagg atctaacaga
     3421 actcgccgtg aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa
     3481 gaaaatcttc gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
     3541 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
     3601 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
     3661 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
     3721 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     3781 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     3841 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     3901 tttggagagg actccggtat ttttacaaca ataccacaac aaaacaaaca acaaacaaca
     3961 ttacaattta ctattctagt cgacctgcag gcggccgcaa tggtgagcaa gggcgaggag
     4021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4681 gccgggatca ctctcggcat ggacgagctg tacaagcagt ggtctctgtc cagtcctggc
     4741 ctcgtcggcc attctcgact aagttggcag catcacccga cgcactttgc gccgaataaa
     4801 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     4861 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     4921 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     4981 caggagctaa ggaagctaaa cttttgctga cgagaacagg gactggtgaa atgcagttta
     5041 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     5101 ttattgacac gcctgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     5161 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     5221 tgaccaccga tatggccagt gtgccggtat ccgttatcgg ggaagaagtg gctgatctca
     5281 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     5341 caggctccct tatacacagg gccagtctgg ccacttgtgg tctgctgaga ccgtgacggc
     5401 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     5461 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     5521 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     5581 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ct
//
