LOCUS       Exported                6666 bp ds-DNA     circular SYN 12-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      50..371
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     regulatory      377..698
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     5'UTR           799..929
                     /label=Translational enhancer
                     /note="translational enhancer 5'-UTR from tobacco etch 
                     virus"
     misc_feature    938..1132
                     /label=CEYFP
     misc_feature    1148..1823
                     /label=NC Frame
     regulatory      1834..2044
                     /regulatory_class="terminator"
                     /note="CaMV 35S terminator"
     misc_feature    2512..2536
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2627..3215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3316..4131)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4422..4857
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4990..5012
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      5036..5287
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5327..6121)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6155..6334)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc catgggcggc agcgtgcagc tcgccgacca
      961 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1021 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1081 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     1141 cagatctcag tggtctctgt ccagtcctgg cctcgtcggc cattctcgac taagttggca
     1201 gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa
     1261 taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa
     1321 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1381 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa acttttgctg
     1441 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     1501 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcctgggcg acggatggtg
     1561 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     1621 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggta
     1681 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     1741 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ggccagtctg
     1801 gccacttgtg gtctgctgag accctgatct agagtccgca aaaatcacca gtctctctct
     1861 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     1921 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     1981 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     2041 tgacgcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg
     2101 gagctccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
     2161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2221 aaghcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2281 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2461 tacggttatc cacagaatca ggggataacg caggaaagaa catgaaggcc ttgacaggat
     2521 atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag
     2581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3061 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg gttacaacca
     3301 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     3361 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     3421 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     3481 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     3541 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     3601 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     3661 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     3721 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     3781 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     3841 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     3901 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     3961 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatccat agattgtcgc
     4021 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     4081 ggaatttaat cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct
     4141 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     4201 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     4261 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     4321 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     4381 cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt
     4441 cgagcgggct tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc
     4501 gctaatgccc cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca
     4561 cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata
     4621 aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc
     4681 aacagcttgc aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta
     4741 gcttttcaat tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg
     4801 ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag
     4861 cgcctttgcc cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt
     4921 gaaaaagaaa aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga
     4981 attagatctt ggcaggatat attgtggtgt aacgttatca gcttgcatgc cggtcgatct
     5041 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     5101 tctatcgcgt attaaatgta taattgcggg actctaatca aaaaacccat ctcataaata
     5161 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     5221 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     5281 aacgatctgc ttgactctag ctagagtccg aaccccagag tcccgctcag aagaactcgt
     5341 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     5401 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     5461 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     5521 cattttccac catgatattc ggcaagcagg catcgccctg ggtcacgacg agatcctcgc
     5581 cgtcgggcat ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     5641 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt cctcgctcga
     5701 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     5761 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     5821 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     5881 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     5941 ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     6001 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     6061 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     6121 tgcctcgatc gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg
     6181 gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga
     6241 cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac
     6301 ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt
     6361 gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctcatgt atcgataaca
     6421 ttaacgttta caatttcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6481 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     6541 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     6601 ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt
     6661 atcgat
//
