LOCUS       Exported                6656 bp ds-DNA     circular SYN 12-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      50..371
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     regulatory      377..698
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     5'UTR           799..929
                     /label=Translational enhancer
                     /note="translational enhancer 5'-UTR from tobacco etch 
                     virus"
     misc_feature    931..1606
                     /label=NC Frame
     misc_feature    1608..1802
                     /label=CEYFP
     regulatory      1824..2034
                     /regulatory_class="terminator"
                     /note="CaMV 35S terminator"
     misc_feature    2502..2526
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2617..3205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3306..4121)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4412..4847
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4980..5002
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      5026..5277
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5317..6111)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6145..6324)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc cagtggtctc tgtccagtcc tggcctcgtc
      961 ggccattctc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     1021 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     1081 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     1141 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1201 ctaaggaagc taaacttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     1261 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     1321 acacgcctgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     1381 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     1441 ccgatatggc cagtgtgccg gtatccgtta tcggggaaga agtggctgat ctcagccacc
     1501 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     1561 cccttataca cagggccagt ctggccactt gtggtctgct gagacccggc agcgtgcagc
     1621 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1681 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1741 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1801 agtgaagatc cacctgatct agagtccgca aaaatcacca gtctctctct acaaatctat
     1861 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     1921 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     1981 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcgaat
     2041 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     2101 ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc atagctgttt
     2161 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aaghcataaa
     2221 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2281 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2341 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2401 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2461 cacagaatca ggggataacg caggaaagaa catgaaggcc ttgacaggat atattggcgg
     2521 gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa
     2581 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     3061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3241 tcagtggaac gaaaactcac gttaagggat tttggtcatg gttacaacca attaaccaat
     3301 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     3361 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     3421 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     3481 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     3541 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     3601 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     3661 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     3721 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     3781 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     3841 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     3901 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     3961 agaaacaact ctggcgcatc gggcttccca tacaatccat agattgtcgc acctgattgc
     4021 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     4081 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     4141 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     4201 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     4261 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     4321 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc
     4381 tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt cgagcgggct
     4441 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
     4501 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
     4561 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
     4621 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
     4681 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
     4741 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
     4801 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
     4861 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
     4921 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt
     4981 ggcaggatat attgtggtgt aacgttatca gcttgcatgc cggtcgatct agtaacatag
     5041 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     5101 attaaatgta taattgcggg actctaatca aaaaacccat ctcataaata acgtcatgca
     5161 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
     5221 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc
     5281 ttgactctag ctagagtccg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     5341 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5401 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5461 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5521 catgatattc ggcaagcagg catcgccctg ggtcacgacg agatcctcgc cgtcgggcat
     5581 ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5641 atcatcctga tcgacaagac cggcttccat ccgagtacgt cctcgctcga tgcgatgttt
     5701 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5761 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     5821 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     5881 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt
     5941 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     6001 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6061 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc
     6121 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     6181 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     6241 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
     6301 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     6361 atcggcgggg gtcataacgt gactccctta attctcatgt atcgataaca ttaacgttta
     6421 caatttcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6481 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6541 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
     6601 actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgat
//
