LOCUS       Exported                4193 bp ds-DNA     circular SYN 14-OCT-2022
DEFINITION  pRec_EF1a-attB(Rec_Kp03)_mTagBFP2-pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 18, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SnapGene NotesSequence Type: NaturalTransformed Into: 
            unspecifiedSnapGene UUID: 
            124d74b4-cb18-46ff-ab4d-d8c2c1a42956Sequence Document Type: 
            UNAImported FromPath: C:\Users\aliso\Dropbox (MCBL-P)\Hsu Lab at 
            Berkeley\Alison sandbox\Recombinase plasmid maps\Round 1 LSRs\DNA 
            FilesFilename: pC476.dna
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        32..136
     CDS             137..997
                     /standard_name="AmpR"
                     /label=AmpR
     rep_origin      1168..1756
     promoter        1941..3118
     misc_feature    3119..3231
                     /label=Kp03 attB
                     /note="Geneious type: regulatory_region"
                     /note="/modified by=aliso"
     CDS             3241..3951
                     /standard_name="mTagBFP2"
                     /label=mTagBFP2
     misc_feature    3985..4192
                     /label=Geneious type: polyA
                     /note="Geneious type: polyA"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2221 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
     2281 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     2341 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     2401 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     2461 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     2521 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     2581 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     2641 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     2701 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     2761 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     2821 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     2881 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     2941 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3001 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3061 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgatt
     3121 tgcatttaaa atcggagcat catttttcaa cagaaacgac tatgagcgca atgacccttg
     3181 atttacctcg ccgctttccg tggccaacgc tgctgtccgt ggctatccac ggccgccacc
     3241 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
     3301 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     3361 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     3421 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     3481 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     3541 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     3601 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     3661 acactcggct gggaggcctt caccgaaacg ctgtaccccg ctgacggcgg cctggaaggc
     3721 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     3781 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     3841 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     3901 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaactcgag
     3961 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4081 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4141 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gac
//
