LOCUS       pEXP5-NT/pT7_Lux        3494 bp    DNA     circular UNA 20-OCT-2022
DEFINITION  T7 RNAP constitutive expression of 6xHis LuxR (E. coli codon
            optimized).
ACCESSION   urn.local.gonzales.iq-fg44w87
VERSION     urn.local.gonzales.iq-fg44w87
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      3474..108
                     /created_by="gonzales"
                     /label="T7"
     gene            517..1176
                     /created_by="gonzales"
                     /label="AmpR"
     rep_origin      1274..1956
                     /created_by="gonzales"
                     /label="ColE1"
     promoter        2567..2585
                     /created_by="gonzales"
                     /label="T7"
     RBS             2634..2639
                     /created_by="gonzales"
                     /label="RBS"
     gene            2646..3461
                     /created_by="gonzales"
                     /label="6xHis-LuxR opt"
     Optimized_Codon 2718..2720
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 2733..2735
                     /Relative_Adaptiveness="1.0"
                     /Original="ACA"
                     /label="ACC"
     Optimized_Codon 2739..2741
                     /Relative_Adaptiveness="1.0"
                     /Original="AGA"
                     /label="CGC"
     Optimized_Codon 2742..2744
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 2745..2747
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 2754..2756
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 2760..2762
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 2766..2768
                     /Relative_Adaptiveness="1.0"
                     /Original="AGA"
                     /label="CGC"
     Optimized_Codon 2778..2780
                     /Relative_Adaptiveness="0.59118"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 2781..2783
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 2793..2795
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 2805..2807
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 2814..2816
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 2832..2834
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 2835..2837
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 2841..2843
                     /Relative_Adaptiveness="1.0"
                     /Original="ATC"
                     /label="ATT"
     Optimized_Codon 2850..2852
                     /Relative_Adaptiveness="1.0"
                     /Original="CCT"
                     /label="CCG"
     Optimized_Codon 2862..2864
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 2877..2879
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 2883..2885
                     /Relative_Adaptiveness="1.0"
                     /Original="CTA"
                     /label="CTG"
     Optimized_Codon 2895..2897
                     /Relative_Adaptiveness="1.0"
                     /Original="CCT"
                     /label="CCG"
     Optimized_Codon 2907..2909
                     /Relative_Adaptiveness="0.95524"
                     /Original="AGG"
                     /label="CGT"
     Optimized_Codon 2925..2927
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 2928..2930
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 2931..2933
                     /Relative_Adaptiveness="0.20922"
                     /Original="TTA"
                     /label="CTT"
     Optimized_Codon 2934..2936
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 2943..2945
                     /Relative_Adaptiveness="0.59118"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 2946..2948
                     /Relative_Adaptiveness="1.0"
                     /Original="CCT"
                     /label="CCG"
     Optimized_Codon 2949..2951
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 2952..2954
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 2976..2978
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 2982..2984
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 2994..2996
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 3009..3011
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 3012..3014
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 3027..3029
                     /Relative_Adaptiveness="1.0"
                     /Original="CCA"
                     /label="CCG"
     Optimized_Codon 3033..3035
                     /Relative_Adaptiveness="0.70064"
                     /Original="GTA"
                     /label="GTT"
     Optimized_Codon 3036..3038
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3051..3053
                     /Relative_Adaptiveness="1.0"
                     /Original="ACA"
                     /label="ACC"
     Optimized_Codon 3054..3056
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 3060..3062
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3063..3065
                     /Relative_Adaptiveness="1.0"
                     /Original="ATC"
                     /label="ATT"
     Optimized_Codon 3066..3068
                     /Relative_Adaptiveness="0.61511"
                     /Original="ACT"
                     /label="ACG"
     Optimized_Codon 3069..3071
                     /Relative_Adaptiveness="1.0"
                     /Original="GGG"
                     /label="GGC"
     Optimized_Codon 3081..3083
                     /Relative_Adaptiveness="0.36686"
                     /Original="CCT"
                     /label="CCA"
     Optimized_Codon 3093..3095
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 3108..3110
                     /Relative_Adaptiveness="0.84475"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 3114..3116
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3129..3131
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 3147..3149
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 3156..3158
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3162..3164
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3165..3167
                     /Relative_Adaptiveness="0.75274"
                     /Original="CAT"
                     /label="CAC"
     Optimized_Codon 3180..3182
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 3183..3185
                     /Relative_Adaptiveness="1.0"
                     /Original="CCA"
                     /label="CCG"
     Optimized_Codon 3186..3188
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3195..3197
                     /Relative_Adaptiveness="1.0"
                     /Original="CCT"
                     /label="CCG"
     Optimized_Codon 3201..3203
                     /Relative_Adaptiveness="1.0"
                     /Original="CTA"
                     /label="CTG"
     Optimized_Codon 3216..3218
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGC"
     Optimized_Codon 3222..3224
                     /Relative_Adaptiveness="0.82162"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 3228..3230
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 3243..3245
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 3252..3254
                     /Relative_Adaptiveness="0.59118"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3255..3257
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3264..3266
                     /Relative_Adaptiveness="1.0"
                     /Original="AGA"
                     /label="CGC"
     Optimized_Codon 3279..3281
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3288..3290
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     Optimized_Codon 3297..3299
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGC"
     Optimized_Codon 3303..3305
                     /Relative_Adaptiveness="0.55618"
                     /Original="AGC"
                     /label="TCG"
     Optimized_Codon 3318..3320
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 3324..3326
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATT"
     Optimized_Codon 3327..3329
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3330..3332
                     /Relative_Adaptiveness="0.84475"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3339..3341
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3342..3344
                     /Relative_Adaptiveness="1.0"
                     /Original="CGT"
                     /label="CGC"
     Optimized_Codon 3345..3347
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 3351..3353
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 3360..3362
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3369..3371
                     /Relative_Adaptiveness="0.75802"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 3381..3383
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3387..3389
                     /Relative_Adaptiveness="1.0"
                     /Original="ACA"
                     /label="ACC"
     Optimized_Codon 3390..3392
                     /Relative_Adaptiveness="1.0"
                     /Original="ACA"
                     /label="ACC"
     Optimized_Codon 3423..3425
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3426..3428
                     /Relative_Adaptiveness="0.61511"
                     /Original="ACA"
                     /label="ACG"
     Optimized_Codon 3429..3431
                     /Relative_Adaptiveness="0.84475"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 3444..3446
                     /Relative_Adaptiveness="1.0"
                     /Original="CCA"
                     /label="CCG"
     Optimized_Codon 3447..3449
                     /Relative_Adaptiveness="1.0"
                     /Original="TAC"
                     /label="TAT"
     Optimized_Codon 3450..3452
                     /Relative_Adaptiveness="0.73943"
                     /Original="TTT"
                     /label="TTC"
     Optimized_Codon 3456..3458
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
ORIGIN      
        1 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
       61 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga
      121 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      181 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      241 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      301 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      361 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      421 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      481 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      541 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      601 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      661 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      721 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      781 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      841 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      901 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      961 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1021 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1081 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1141 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1201 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1261 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1321 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1381 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1441 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1501 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1561 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1621 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1681 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1741 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1801 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1861 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1921 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1981 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2041 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2101 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2161 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2221 agtatacact ccgctatcgc tacgcttcgt taatacagat gtaggtgttc cacagggtag
     2281 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     2341 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     2401 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     2461 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     2521 ccgtggccag gacccaacgc tgcccgagat ctcgatcccg cgaaattaat acgactcact
     2581 atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     2641 ataccatgtc tggttctcat catcatcatc atcatggtag cagcggcgaa aacctgtatt
     2701 ttcagtccat gaaaaacatc aatgccgacg acacctaccg cattatcaat aaaatcaaag
     2761 cgtgtcgcag caataatgac atcaatcaat gcctgtctga tatgaccaaa atggtgcatt
     2821 gtgaatatta tctgctggcg attatttatc cgcattctat ggtgaaatct gatattagca
     2881 ttctggataa ttacccgaaa aaatggcgtc aatattatga tgacgcgaac cttatcaaat
     2941 atgacccgat tgtggattat tctaactcca atcatagccc aatcaattgg aatatttttg
     3001 aaaacaatgc ggtgaataaa aaatctccga atgttatcaa agaagcgaaa accagcggtc
     3061 tgattacggg ctttagtttc ccaattcata cggcgaacaa tggcttcggt atgctgagtt
     3121 ttgcacatag cgaaaaagac aactatattg atagtctgtt tctgcacgcg tgtatgaaca
     3181 ttccgctgat tgttccgtct ctggttgata attatcgcaa aatcaatatt gcaaataata
     3241 aaagcaacaa cgacctgacc aaacgcgaaa aagaatgtct ggcgtgggcg tgcgaaggca
     3301 aatcgtcttg ggatattagc aaaattctgg gttgcagtga acgcaccgtc accttccatc
     3361 tgaccaatgc ccaaatgaaa ctgaatacca ccaaccgctg ccaaagtatt tctaaagcaa
     3421 ttctgacggg tgcaattgat tgcccgtatt tcaaaaacta aagggtgatc cggctgctaa
     3481 caaagcccga aagg
//
