LOCUS       pEXP5-NT/pT7_Lux        3356 bp    DNA     circular UNA 20-OCT-2022
DEFINITION  T7 RNAP constitutive expression of LuxI (E. coli codon optimized).
ACCESSION   urn.local.gonzales.ia-fg44vng
VERSION     urn.local.gonzales.ia-fg44vng
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    183..188
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     gene            517..1176
                     /created_by="gonzales"
                     /label="AmpR"
     rep_origin      1274..1956
                     /created_by="gonzales"
                     /label="ColE1"
     promoter        2567..2585
                     /created_by="gonzales"
                     /label="T7"
     misc_feature    2605..2610
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     RBS             2634..2639
                     /created_by="gonzales"
                     /label="RBS"
     gene            2646..3323
                     /created_by="gonzales"
                     /modified_by="gonzales"
                     /label="6xHis LuxI opt"
     terminator      3336..108
                     /created_by="gonzales"
                     /label="T7"
ORIGIN      
        1 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
       61 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga
      121 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      181 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      241 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      301 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      361 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      421 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      481 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      541 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      601 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      661 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      721 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      781 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      841 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      901 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      961 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1021 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1081 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1141 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1201 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1261 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1321 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1381 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1441 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1501 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1561 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1621 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1681 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1741 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1801 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1861 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1921 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1981 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2041 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2101 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2161 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2221 agtatacact ccgctatcgc tacgcttcgt taatacagat gtaggtgttc cacagggtag
     2281 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     2341 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     2401 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     2461 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     2521 ccgtggccag gacccaacgc tgcccgagat ctcgatcccg cgaaattaat acgactcact
     2581 atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     2641 ataccatgtc tggttctcat catcatcatc atcatggtag cagcggcgaa aacctgtatt
     2701 ttcagtccat gaccattatg attaaaaaat cggattttct ggcaattccg tcggaagaat
     2761 ataaaggtat tctgagtctg cgttatcaag tgtttaaaca acgcctggaa tgggacctgg
     2821 ttgtggaaaa taacctggaa agcgatgaat atgataacag caatgcagaa tatatttatg
     2881 cgtgtgatga taccgaaaat gtgagtggct gctggcgtct gctgccgacc accggtgatt
     2941 atatgctgaa aagtgttttt ccggaactgc tgggtcaaca gagtgcgccg aaagatccga
     3001 atattgtcga actgagtcgt tttgcggtgg gtaaaaatag cagcaaaatt aataactctg
     3061 cgagtgaaat taccatgaaa ctgtttgaag cgatttataa acacgcggtt agtcaaggta
     3121 ttaccgaata tgtgaccgtg accagcaccg caattgaacg ctttctgaaa cgtattaaag
     3181 ttccgtgtca tcgtattggc gacaaagaaa ttcatgtgct gggtgatacc aaatcggttg
     3241 tgctgtctat gccgattaat gaacagttta aaaaagcagt cctgaatgca gcgaacgacg
     3301 aaaattacgc cctggcagcg taaagggtga tccggctgct aacaaagccc gaaagg
//
