LOCUS       pEXP5-NT/pLux_La        3444 bp    DNA     circular UNA 20-OCT-2022
DEFINITION  LuxR:3OC6 HSL inducible promoter to express 6xHis LasI (E. coli
            codon optimized).
ACCESSION   urn.local.gonzales.2d-fg3tmaf
VERSION     urn.local.gonzales.2d-fg3tmaf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        86..154
                     /created_by="gonzales"
                     /label="plux"
     regulatory      106..125
                     /created_by="gonzales"
                     /label="Lux operator"
     RBS             184..189
                     /created_by="gonzales"
                     /label="RBS"
     gene            196..864
                     /created_by="gonzales"
                     /label="6xHis LasIopt"
     Optimized_Codon 277..279
                     /Relative_Adaptiveness="1.0"
                     /Original="CGG"
                     /label="CGC"
     Optimized_Codon 322..324
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 403..405
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 421..423
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 466..468
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGC"
     Optimized_Codon 472..474
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 487..489
                     /Relative_Adaptiveness="1.0"
                     /Original="CCC"
                     /label="CCG"
     Optimized_Codon 517..519
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 562..564
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 586..588
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGC"
     Optimized_Codon 784..786
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 796..798
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 820..822
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 829..831
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGC"
     Optimized_Codon 847..849
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGC"
     terminator      877..1005
                     /created_by="gonzales"
                     /label="T7"
     gene            1414..2073
                     /created_by="gonzales"
                     /label="AmpR"
     rep_origin      2171..2853
                     /created_by="gonzales"
                     /label="ColE1 Origin"
ORIGIN      
        1 gatctgagct ggcgtacgcg ttgaacactt cacagatgat agggattcgg gtaaagagcg
       61 tgtcattggg ggcttataca ggcgttacaa ttacttaaca taagcacctg taggatcgta
      121 caggtttacg caagaaaatg gtttgttata gtcgtctaga aataattttg tttaacttta
      181 agaaggagat ataccatgtc tggttctcat catcatcatc atcatggtag cagcggcgaa
      241 aacctgtatt ttcagtccat gatcgtacaa attggtcgcc gcgaagagtt cgataaaaaa
      301 ctgctgggcg agatgcacaa gctgcgtgct caagtgttca aggagcgcaa aggctgggac
      361 gttagtgtca tcgacgagat ggaaatcgat ggttatgacg cactgagtcc ttattacatg
      421 ctgatccagg aagatactcc tgaagcccag gttttcggtt gctggcgcat tctggatacc
      481 actggcccgt acatgctgaa gaacaccttc ccggagctgc tgcacggcaa ggaagcgcct
      541 tgctcgccgc acatctggga actgagccgt ttcgccatca actctggcca gaaaggctcg
      601 ctgggctttt ccgactgtac gctggaggcg atgcgcgcgc tggcccgcta cagcctgcag
      661 aacgacatcc agacgctggt gacggtaacc accgtaggcg tggagaagat gatgatccgt
      721 gccggcctgg acgtatcgcg cttcggtccg cacctgaaga tcggcatcga gcgcgcggtg
      781 gccctgcgca tcgaactgaa tgccaagacc cagatcgcgc tgtacggggg cgtgctggtg
      841 gaacagcgcc tggcggtttc atgaagggtg atccggctgc taacaaagcc cgaaaggaag
      901 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      961 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataattc ttgaagacga
     1021 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1081 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1441 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     1501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3001 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3061 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     3121 atacactccg ctatcgctac gcttcgttaa tacagatgta ggtgttccac agggtagcca
     3181 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     3241 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     3301 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     3361 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     3421 tggccaggac ccaacgctgc ccga
//
