LOCUS       pEXP5-NT/pLas_eG        3671 bp    DNA     circular UNA 20-OCT-2022
DEFINITION  LasR:3OC12 HSL inducible promoter to express 6xHis eGFP.
ACCESSION   urn.local.gonzales.2c-fg3tmaf
VERSION     urn.local.gonzales.2c-fg3tmaf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        86..189
                     /created_by="gonzales"
                     /label="pLas"
     RBS             219..224
                     /created_by="gonzales"
                     /label="RBS"
     gene            231..1091
                     /created_by="gonzales"
                     /label="eGFP"
     terminator      1104..1232
                     /created_by="gonzales"
                     /label="T7"
     gene            1641..2300
                     /created_by="gonzales"
                     /label="AmpR"
     rep_origin      2398..3080
                     /created_by="gonzales"
                     /label="ColE1 Origin"
ORIGIN      
        1 gatctgagct ggcgtacgcg ttgaacactt cacagatgat agggattcgg gtaaagagcg
       61 tgtcattggg ggcttataca ggcgttgctg ttccgctggg catgcttcga gcctagcaag
      121 ggtccgggtt caccgaaatc tatctcattt gctagttata aaattatgaa atttgcgtaa
      181 attcttcagt ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgtctggtt
      241 ctcatcatca tcatcatcat ggtagcagcg gcgaaaacct gtattttcag tcccttgtgg
      301 gtaccggttc gaatgatgac gacgacaaga gtccggatcc caattggatg gtgagcaagg
      361 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      421 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      481 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      541 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      601 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      661 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      721 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      781 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      841 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      901 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      961 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1021 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     1081 gagtctggta aagggtgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     1141 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     1201 ttttttgctg aaaggaggaa ctatatccgg ataattcttg aagacgaaag ggcctcgtga
     1261 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1681 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2581 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
     3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     3361 tcgctacgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     3421 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3481 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3541 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3601 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     3661 acgctgcccg a
//
