LOCUS       pEXP5-NT/pT7_Las        3347 bp    DNA     circular UNA 21-OCT-2022
DEFINITION  T7 RNAP constitutive expression of 6xHis LasI (E. coli codon
            optimized).
ACCESSION   urn.local.gonzales.h5-fg44uk4
VERSION     urn.local.gonzales.h5-fg44uk4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      3327..108
                     /created_by="gonzales"
                     /label="T7"
     gene            517..1176
                     /created_by="gonzales"
                     /label="AmpR"
     rep_origin      1274..1956
                     /created_by="gonzales"
                     /label="ColE1"
     promoter        2567..2585
                     /created_by="gonzales"
                     /label="T7"
     RBS             2634..2639
                     /created_by="gonzales"
                     /label="RBS"
     gene            2646..3314
                     /created_by="gonzales"
                     /label="6xHis LasIopt"
     Optimized_Codon 2727..2729
                     /Relative_Adaptiveness="1.0"
                     /Original="CGG"
                     /label="CGC"
     Optimized_Codon 2772..2774
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 2853..2855
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 2871..2873
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 2916..2918
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGC"
     Optimized_Codon 2922..2924
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 2937..2939
                     /Relative_Adaptiveness="1.0"
                     /Original="CCC"
                     /label="CCG"
     Optimized_Codon 2967..2969
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3012..3014
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3036..3038
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGC"
     Optimized_Codon 3234..3236
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 3246..3248
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3270..3272
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3279..3281
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGC"
     Optimized_Codon 3297..3299
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGC"
ORIGIN      
        1 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
       61 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga
      121 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      181 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      241 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      301 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      361 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      421 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      481 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      541 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      601 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      661 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      721 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      781 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      841 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      901 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      961 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1021 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1081 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1141 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1201 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1261 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1321 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1381 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1441 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1501 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1561 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1621 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1681 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1741 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1801 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1861 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1921 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1981 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2041 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2101 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2161 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2221 agtatacact ccgctatcgc tacgcttcgt taatacagat gtaggtgttc cacagggtag
     2281 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     2341 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     2401 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     2461 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     2521 ccgtggccag gacccaacgc tgcccgagat ctcgatcccg cgaaattaat acgactcact
     2581 atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     2641 ataccatgtc tggttctcat catcatcatc atcatggtag cagcggcgaa aacctgtatt
     2701 ttcagtccat gatcgtacaa attggtcgcc gcgaagagtt cgataaaaaa ctgctgggcg
     2761 agatgcacaa gctgcgtgct caagtgttca aggagcgcaa aggctgggac gttagtgtca
     2821 tcgacgagat ggaaatcgat ggttatgacg cactgagtcc ttattacatg ctgatccagg
     2881 aagatactcc tgaagcccag gttttcggtt gctggcgcat tctggatacc actggcccgt
     2941 acatgctgaa gaacaccttc ccggagctgc tgcacggcaa ggaagcgcct tgctcgccgc
     3001 acatctggga actgagccgt ttcgccatca actctggcca gaaaggctcg ctgggctttt
     3061 ccgactgtac gctggaggcg atgcgcgcgc tggcccgcta cagcctgcag aacgacatcc
     3121 agacgctggt gacggtaacc accgtaggcg tggagaagat gatgatccgt gccggcctgg
     3181 acgtatcgcg cttcggtccg cacctgaaga tcggcatcga gcgcgcggtg gccctgcgca
     3241 tcgaactgaa tgccaagacc cagatcgcgc tgtacggggg cgtgctggtg gaacagcgcc
     3301 tggcggtttc atgaagggtg atccggctgc taacaaagcc cgaaagg
//
