LOCUS       Exported                9538 bp ds-DNA     circular SYN 03-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-CMV-human MYCN-EF1-mRFP1  4-25-22
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 21, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    181..186
                     /label=EcoRI
     misc_feature    196..336
                     /label=5' UTR hMYCN  BC002712
     CDS             337..1731
                     /codon_start=1
                     /label=Human MYCN  CDS  B002712
                     /translation="MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPP
                     GEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGG
                     LTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHG
                     GAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASG
                     AGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVT
                     VEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVE
                     SEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRS
                     SFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKI
                     EHARTC"
     misc_feature    1732..2226
                     /label=3' UTR hMYCN  BC002712
     misc_feature    2230..2235
                     /label=EcoRI
     promoter        2293..2504
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             2517..2785
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     CDS             2819..3493
                     /codon_start=1
                     /label=mRFP1
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=96.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERFMNFEDGGV
                     VTVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYKAKKQVQLPGAYMTDIKLDIISHNGDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    3506..4093
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4088..4195
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=69.4"
                     /note="/match_length=13.7"
                     /note="/other=CDS"
                     /translation="PRLVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRR"
     LTR             4220..4400
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    4472..4593
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      4633..4768
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     protein_bind    complement(4825..4841)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(4849..4879)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(4894..4915)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4928..5020)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(5203..5791)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5962..6822)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6823..6927)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             7022..7057
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    7159..7258
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
     CDS             complement(7264..7418)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     promoter        7432..7658
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             7659..7839
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    7886..8011
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             7995..8333
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVR"
     misc_feature    8504..8737
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             9114..9214
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    9233..9349
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     enhancer        9362..9438
                     /label=CMV enhancer (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.3"
                     /note="/other=enhancer"
     promoter        join(9439..9538,1..104)
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
       61 tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta gtgaaccgtc
      121 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc tagagctagc
      181 gaattcggca cgaggctccg gtgtgtctgt cggttgcagt gttggaggtc ggcgccggcc
      241 cccgccttcc gcgcccccca cgggaaggaa gcacccccgg tattaaaacg aacggggcgg
      301 aaagaagccc tcagtcgccg gccgggaggc gagccgatgc cgagctgctc cacgtccacc
      361 atgccgggca tgatctgcaa gaacccagac ctcgagtttg actcgctaca gccctgcttc
      421 tacccggacg aagatgactt ctacttcggc ggccccgact cgaccccccc gggggaggac
      481 atctggaaga agtttgagct gctgcccacg cccccgctgt cgcccagccg tggcttcgcg
      541 gagcacagct ccgagccccc gagctgggtc acggagatgc tgcttgagaa cgagctgtgg
      601 ggcagcccgg ccgaggagga cgcgttcggc ctggggggac tgggtggcct cacccccaac
      661 ccggtcatcc tccaggactg catgtggagc ggcttctccg cccgcgagaa gctggagcgc
      721 gccgtgagcg agaagctgca gcacggccgc gggccgccaa ccgccggttc caccgcccag
      781 tccccgggag ccggcgccgc cagccctgcg ggtcgcgggc acggcggggc tgcgggagcc
      841 ggccgcgccg gggccgccct gcccgccgag ctcgcccacc cggccgccga gtgcgtggat
      901 cccgccgtgg tcttcccctt tcccgtgaac aagcgcgagc cagcgcccgt gcccgcagcc
      961 ccggccagtg ccccggcggc gggccctgcg gtcgcctcgg gggcgggtat tgccgcccca
     1021 gccggggccc cgggggtcgc ccctccgcgc ccaggcggcc gccagaccag cggcggcgac
     1081 cacaaggccc tcagtacctc cggagaggac accctgagcg attcagatga tgaagatgat
     1141 gaagaggaag atgaagagga agaaatcgac gtggtcactg tggagaagcg gcgttcctcc
     1201 tccaacacca aggctgtcac cacattcacc atcactgtgc gtcccaagaa cgcagccctg
     1261 ggtcccggga gggctcagtc cagcgagctg atcctcaaac gatgccttcc catccaccag
     1321 cagcacaact atgccgcccc ctctccctac gtggagagtg aggatgcacc cccacagaag
     1381 aagataaaga gcgaggcgtc cccacgtccg ctcaagagtg tcatcccccc aaaggctaag
     1441 agcttgagcc cccgaaactc tgactcggag gacagtgagc gtcgcagaaa ccacaacatc
     1501 ctggagcgcc agcgccgcaa cgaccttcgg tccagctttc tcacgctcag ggaccacgtg
     1561 ccggagttgg taaagaatga gaaggccgcc aaggtggtca ttttgaaaaa ggccactgag
     1621 tatgtccact ccctccaggc cgaggagcac cagcttttgc tggaaaagga aaaattgcag
     1681 gcaagacagc agcagttgct aaagaaaatt gaacacgctc ggacttgcta gacgcttctc
     1741 aaaactggac agtcactgcc actttgcaca ttttgatttt ttttttaaac aaacattgtg
     1801 ttgacattaa gaatgttggt ttactttcaa atcggtcccc tgtcgagttc ggctctgggt
     1861 gggcagtagg accaccagtg tggggttctg ctgggacctt ggagagcctg catcccagga
     1921 tgctgggtgg ccctgcagcc tcctccacct cacctccatg acagcgctaa acgttggtga
     1981 cggttgggag cctctggggc tgttgaagtc accttgtgtg ttccaagttt ccaaacaaca
     2041 gaaagtcatt ccttcttttt aaaatggtgc ttaagttcca gcagatgcca cataaggggt
     2101 ttgccatttg atacccctgg ggaacatttc tgtaaatacc attgacacat ccgccttttg
     2161 tatacatcct gggtaatgag aggtggcttt tgcggccagt attagactgg aagttcatac
     2221 ctaagtcctg aattcgaatt taaatcggat ccgcggccgc aaggatctgc gatcgctccg
     2281 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2341 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2401 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2461 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     2521 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     2581 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     2641 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     2701 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     2761 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgata tcgccaccat
     2821 ggccagctcc gaggatgtca tcaaagagtt tatgagattt aaggtcaaga tggagggaag
     2881 cgtcaacgga cacgagttcg agattgaggg agaaggagaa ggccggcctt acgagggcac
     2941 acaaaccgct aagctcaagg tcacaaaagg aggacccctc cccttctcct gggatattct
     3001 gagccctcag ttccagtacg gaagcaaagc ctatgttaaa caccctgccg acatccctga
     3061 ctatctgaag ctctccttcc ctgaaggctt caagtgggag agattcatga acttcgagga
     3121 cggaggcgtg gtgacagtca cacaagatag caccctccag gacggagagt ttatttataa
     3181 ggtgaaactc agaggaacca acttcccctc cgatggccct gtcatgcaaa aaaaaacaat
     3241 gggatgggaa gcctccaccg agagaatgta tcctgaggat ggcgctctga aaggcgaaat
     3301 taaaatgaga ctgaaactca aagacggagg acactacgat gccgaggtca aaacaaccta
     3361 caaggccaag aaacaagtgc agctgcctgg cgcctacatg actgatatta aactcgacat
     3421 tatcagccat aatggggact acaccatcgt ggaacaatat gagagagctg agggcagaca
     3481 tagcacaggc gctggataag tcgacaatca acctctgatt acaaaatttg tgaaagattg
     3541 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3601 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3661 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3721 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3781 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3841 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3901 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3961 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgc cctgctgccg
     4021 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4081 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     4141 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     4201 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4261 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4321 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4381 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     4441 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     4501 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4561 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     4621 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4681 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4741 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     4801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6961 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7021 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     7081 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     7141 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     7201 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7261 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7321 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7381 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgacgc gtgtagtctt
     7441 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7501 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     7561 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7621 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7681 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7741 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7801 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7861 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7921 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7981 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8041 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8101 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8161 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     8221 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8281 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8341 acagcaagcg gccactgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     8401 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8461 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8521 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8581 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8641 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8701 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8761 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8821 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8881 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8941 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9001 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9061 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9121 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     9181 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     9241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9301 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgatactag
     9361 tattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     9481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgtttt
//
