LOCUS       Wulf_H2W_1_pB80-hsKIF5B 8525 bp ds-DNA     circular     04-NOV-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     intron          1..968
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="95.4"
                     /fragment="False"
                     /other="intron"
     misc_feature    1032..2711
                     /label="hsKIF5B(1-560)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1032..3524
                     /label="hsKIF5B(1-560)-L-EGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKSRGGGGSGGGGSGGGGSGGGGSGGGGSGGGSKLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     linker          2718..2804
                     /label="linker 1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2811..3524
                     /label="EGFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     polyA_signal    complement(3623..3814)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    3684..3805
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_binding    complement(3854..3876)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(3880..3910)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(4216..4898)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4234..4822)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(4993..5853)
                     /label="Amp\(r)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(4996..5655)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6292..6360)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     6431..6448
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
       61 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      121 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      181 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      241 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      301 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
      361 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
      421 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg
      901 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcgggg tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg
     1021 ggCGCGCCAC Catggcggac ctggccgagt gcaacatcaa agtgatgtgt cgcttcagac
     1081 ctctcaacga gtctgaagtg aaccgcggcg acaagtacat cgccaagttt cagggagaag
     1141 acacggtcgt gatcgcgtcc aagccttatg catttgatcg ggtgttccag tcaagcacat
     1201 ctcaagagca agtgtataat gactgtgcaa agaagattgt taaagatgta cttgaaggat
     1261 ataatggaac aatatttgca tatggacaaa catcctctgg gaagacacac acaatggagg
     1321 gtaaacttca tgatccagaa ggcatgggaa ttattccaag aatagtgcaa gatattttta
     1381 attatattta ctccatggat gaaaatttgg aatttcatat taaggtttca tattttgaaa
     1441 tatatttgga taagataagg gacctgttag atgtttcaaa gaccaacctt tcagttcatg
     1501 aagacaaaaa ccgagttccc tatgtaaagg ggtgcacaga gcgttttgta tgtagtccag
     1561 atgaagttat ggataccata gatgaaggaa aatccaacag acatgtagca gttacaaata
     1621 tgaatgaaca tagctctagg agtcacagta tatttcttat taatgtcaaa caagagaaca
     1681 cacaaacgga acaaaagctg agtggaaaac tttatctggt tgatttagct ggtagtgaaa
     1741 aggttagtaa aactggagct gaaggtgctg tgctggatga agctaaaaac atcaacaagt
     1801 cactttctgc tcttggaaat gttatttctg ctttggctga gggtagtaca tatgttccat
     1861 atcgagatag taaaatgaca agaatccttc aagattcatt aggtggcaac tgtagaacca
     1921 ctattgtaat ttgctgctct ccatcatcat acaatgagtc tgaaacaaaa tctacactct
     1981 tatttggcca aagggccaaa acaattaaga acacagtttg tgtcaatgtg gagttaactg
     2041 cagaacagtg gaaaaagaag tatgaaaaag aaaaagaaaa aaataagatc ctgcggaaca
     2101 ctattcagtg gcttgaaaat gagctcaaca gatggcgtaa tggggagacg gtgcctattg
     2161 atgaacagtt tgacaaagag aaagccaact tggaagcttt cacagtggat aaagatatta
     2221 ctcttaccaa tgataaacca gcaaccgcaa ttggagttat aggaaatttt actgatgctg
     2281 aaagaagaaa gtgtgaagaa gaaattgcta aattatacaa acagcttgat gacaaggatg
     2341 aagaaattaa ccagcaaagt caactggtag agaaactgaa gacgcaaatg ttggatcagg
     2401 aggagctttt ggcatctacc agaagggatc aagacaatat gcaagctgag ctgaatcgcc
     2461 ttcaagcaga aaatgatgcc tctaaagaag aagtgaaaga agttttacag gccctagaag
     2521 aacttgctgt caattatgat cagaagtctc aggaagttga agacaaaact aaggaatatg
     2581 aattgcttag tgatgaattg aatcagaaat cggcaacttt agcgagtata gatgctgagc
     2641 ttcagaaact taaggaaatg accaaccacc agaaaaaacg agcagctgag atgatggcat
     2701 ctttactaaa atctagaggt ggcgggggca gtgggggagg gggaagtgga ggcggcggtt
     2761 ctggcggggg aggctccggt ggcgggggga gcgggggtgg gagtaagctt gtgagcaagg
     2821 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2881 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2941 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3001 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3061 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3121 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcattg
     3181 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3241 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3301 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3361 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3421 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3481 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggctagc ggaggcggtt
     3541 gaggctcagg cacgcgtacc ggtcctagat aagtaatgat cataatcagc catatcacat
     3601 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3661 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3781 tgtccaaact catcaatgta tcttatcatg tctggatcaa ttcgtaatca tgtcatagct
     3841 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3901 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3961 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4021 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4081 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5101 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     6001 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     6061 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6121 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6181 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6241 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     6301 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     6361 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6421 gtcacgacgt tgtaaaacga cggccagtgc caagttggga tctttgcatt ggcccacggc
     6481 tctcaggatg gggatgctcc ccttcagcac ccggttcccc ttggaaactg atggtcctgg
     6541 ctctgtggca tggcagtggc actgtgagga gccccctacc agcagcacac agtggggttg
     6601 gcactgccac gctccggatg ccgcgctctg atccaacccc ataatcaagg gaacccgaat
     6661 tgccccatca ttgcccccac cacccccatc ctgccgggcc ctcacacccc acgctgcctt
     6721 ctggtgacat tccccagccc aaacccacgg cttcatggct accgcggggc atttcccatt
     6781 gccgccccat tatcagctct gcacacctcc cgctgtaccc atgcctcgtg gctgcccttc
     6841 tttgacgtat aatcttctaa ttaatacccg gccttgtcaa agtggagcac aaacgttaat
     6901 taattcccca gcaggcaggt aattaacagt gtgactccct ttttgctgcg agtggggctg
     6961 atacagagag atgtggcact atggagccca cggggtcctg gcactgggtg cccacggagg
     7021 tccccatgtg ctgcagtgtc accgcctccg aggagacagt attgtccctg cggtgtccct
     7081 gcagctcagc tctgtccaca gggccacctc cagtttggag gggacacaat gcagccccga
     7141 tgcaacccat cctcgcagca tcccagggac aaagacccca ctgcaagacc gcacacaggg
     7201 ctgggtcccg ctcccctaat atctacagtg cttcttgcat ggccccttaa tcaatgcagt
     7261 taatcagcat gcgctcatgc accgctctgg agctgcaaag cccctcgcag cgctgctcac
     7321 caacaccgcg caccgccccg gcccagcctg cagcacgcgc tgcaaacagg aaagaaacaa
     7381 aatattgccc aaatgtaggc aaaggcattc ggctgccttg acctccgccg ggccgggccc
     7441 tgcctgactc agctccttac tcagcgctcg cttcctccct ccggctgcca ccgccgcagc
     7501 gcacaccctg acaaagagtg gcccttaacg ggctctgagg tgcacccagc agtgcactca
     7561 gcagtccaag ggccggcctg gaggtttgca ccgctacgtg ctgacattag cattgaactt
     7621 ggccctgggt agtgctgcag gccgggcggg gtgggtgtag agagtgcagc gcgcgttgca
     7681 cccggtgccc cttcccctcc cttgcatccc agcaggctgc accccagcac caggcccgtg
     7741 catgcatgct cctggtgtta ttgcagcctg gtgcatgcat gcgtcttgat ggtgcagcgc
     7801 tgtgcatgca tcctccttgg tgtgtagcag tctagtgcat gcatacccct cggtgttatt
     7861 gctgctctgt gcacgcacgc tcattgtatc acttcatccc agtgcatgca ctcacactgg
     7921 agcgattgct gctcggtgca cgcacactca ttgtatcact gcagctcagt ggctgcacgc
     7981 acaccggtgt tattgctgct cggtgcgtgc atgcacatca gtgtcgctgc agctcagtgc
     8041 atgcacgctc attgcccatc gctatccctg cctctcctgc tggcgctccc cgggaggtga
     8101 cttcaagggg accgcaggac cacctcgggg gtggggggag ggctgcacac gcggaccccg
     8161 ctccccctcc ccaacaaagc actgtggaat caaaaagggg ggagggggga tggaggggcg
     8221 cgtcacaccc ccgccccaca ccctcacctc gaggtgagcc ccacgttctg cttcactctc
     8281 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     8341 gcagcgatgg gggcgggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     8401 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     8461 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     8521 gcggg
//