LOCUS       Exported                8513 bp ds-DNA     circular SYN 06-JAN-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     KJ103_pb80-hKif5b(1-560)-L-GFP-STOP from 1 to 8500
            pb80-hKif5b(1-560)-L-mCherry-STOP from 1 to 8491
            hKif5b(1-560)_mcherry_2xFKBP_MB from 1 to 9235
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..1018
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label=source:recombinant plasmid
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:recombinant plasmid"
                     /note="color: #ffc0cb"
     source          1019..1021
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label=source:recombinant plasmid
                     /label=source:recombinant plasmid(1)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:recombinant plasmid(1)"
                     /note="color: #ffc0cb"
     source          3515..8513
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label=source:recombinant plasmid
                     /label=source:recombinant plasmid(2)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:recombinant plasmid(2)"
                     /note="color: #ffc0cb"
     primer_bind     861..882
                     /locus_tag="b-actin_fwd"
                     /label=b-actin_fwd
                     /note="color: #14c0bd; direction: RIGHT"
     primer_bind     936..958
                     /locus_tag="pB80 fwd"
                     /label=pB80 fwd
                     /note="color: #14c0bd; direction: RIGHT"
     primer_bind     1011..1018
                     /locus_tag="MB13_Fwd"
                     /label=MB13_Fwd
                     /note="/ApEinfo_hidden="
                     /note="color: #14c0bd; direction: RIGHT"
     misc_feature    1016..1021
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
                     /note="color: #c0c0c0"
     primer_bind     1019..1021
                     /locus_tag="MB13_Fwd(1)"
                     /label=MB13_Fwd
                     /label=MB13_Fwd(1)
                     /note="/ApEinfo_hidden="
                     /note="color: #14c0bd; direction: RIGHT"
     misc_feature    1022..2701
                     /locus_tag="hKif5b(1-560)-rigor"
                     /label=hKif5b(1-560)-rigor
                     /note="color: #ff8040"
     misc_feature    1060..1078
                     /locus_tag="KJ-P139 rev sequencing"
                     /label=KJ-P139 rev sequencing
                     /note="color: #00ffff"
     misc_feature    1722
                     /locus_tag="was GGT now GCT for G-A mutation"
                     /label=was GGT now GCT for G-A mutation
                     /note="color: #00ffff"
     misc_feature    2441..2460
                     /locus_tag="Fwd seq primer KJ-P017"
                     /label=Fwd seq primer KJ-P017
                     /note="color: #00ffff"
     misc_feature    2699..2701
                     /locus_tag="residue 560"
                     /label=residue 560
                     /note="color: #ffff00"
     CDS             2708..2794
                     /locus_tag="linker\1"
                     /label=linker\1
                     /note="/vntifkey=4"
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    2801..3514
                     /locus_tag="mVenus"
                     /label=mVenus
                     /note="color: #ffff00"
     misc_feature    3515..3517
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #c0c0c0"
     primer_bind     complement(3576..3597)
                     /locus_tag="B-actin_Rev"
                     /label=B-actin_Rev
                     /note="color: #4ec02b; direction: LEFT"
     primer_bind     3617..3640
                     /locus_tag="pB80 rev"
                     /label=pB80 rev
                     /note="color: #14c0bd; direction: RIGHT"
     CDS             4982..5842
                     /codon_start=1
                     /locus_tag="Amp (r)"
                     /label=Amp (r)
                     /note="color: #e9d024"
                     /translation="LPMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCND
                     TARPTLTGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCH
                     CYRHRGVTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGY
                     GSTA*FSYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAHH
                     WKTFFGAKTLKDLTAVEIQFDVTHSCTQLIFSIFYFHQRFWVSKNRKAKCRKKGNKGDT
                     EMLNTH"
     promoter        6383..8513
                     /locus_tag="B-actin promoter"
                     /label=B-actin promoter
                     /note="color: #346ee0"
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac
     1021 catggcggac ctggccgagt gcaacatcaa agtgatgtgt cgcttcagac ctctcaacga
     1081 gtctgaagtg aaccgcggcg acaagtacat cgccaagttt cagggagaag acacggtcgt
     1141 gatcgcgtcc aagccttatg catttgatcg ggtgttccag tcaagcacat ctcaagagca
     1201 agtgtataat gactgtgcaa agaagattgt taaagatgta cttgaaggat ataatggaac
     1261 aatatttgca tatggacaaa catcctctgg gaagacacac acaatggagg gtaaacttca
     1321 tgatccagaa ggcatgggaa ttattccaag aatagtgcaa gatattttta attatattta
     1381 ctccatggat gaaaatttgg aatttcatat taaggtttca tattttgaaa tatatttgga
     1441 taagataagg gacctgttag atgtttcaaa gaccaacctt tcagttcatg aagacaaaaa
     1501 ccgagttccc tatgtaaagg ggtgcacaga gcgttttgta tgtagtccag atgaagttat
     1561 ggataccata gatgaaggaa aatccaacag acatgtagca gttacaaata tgaatgaaca
     1621 tagctctagg agtcacagta tatttcttat taatgtcaaa caagagaaca cacaaacgga
     1681 acaaaagctg agtggaaaac tttatctggt tgatttagct gctagtgaaa aggttagtaa
     1741 aactggagct gaaggtgctg tgctggatga agctaaaaac atcaacaagt cactttctgc
     1801 tcttggaaat gttatttctg ctttggctga gggtagtaca tatgttccat atcgagatag
     1861 taaaatgaca agaatccttc aagattcatt aggtggcaac tgtagaacca ctattgtaat
     1921 ttgctgctct ccatcatcat acaatgagtc tgaaacaaaa tctacactct tatttggcca
     1981 aagggccaaa acaattaaga acacagtttg tgtcaatgtg gagttaactg cagaacagtg
     2041 gaaaaagaag tatgaaaaag aaaaagaaaa aaataagatc ctgcggaaca ctattcagtg
     2101 gcttgaaaat gagctcaaca gatggcgtaa tggggagacg gtgcctattg atgaacagtt
     2161 tgacaaagag aaagccaact tggaagcttt cacagtggat aaagatatta ctcttaccaa
     2221 tgataaacca gcaaccgcaa ttggagttat aggaaatttt actgatgctg aaagaagaaa
     2281 gtgtgaagaa gaaattgcta aattatacaa acagcttgat gacaaggatg aagaaattaa
     2341 ccagcaaagt caactggtag agaaactgaa gacgcaaatg ttggatcagg aggagctttt
     2401 ggcatctacc agaagggatc aagacaatat gcaagctgag ctgaatcgcc ttcaagcaga
     2461 aaatgatgcc tctaaagaag aagtgaaaga agttttacag gccctagaag aacttgctgt
     2521 caattatgat cagaagtctc aggaagttga agacaaaact aaggaatatg aattgcttag
     2581 tgatgaattg aatcagaaat cggcaacttt agcgagtata gatgctgagc ttcagaaact
     2641 taaggaaatg accaaccacc agaaaaaacg agcagctgag atgatggcat ctttactaaa
     2701 atctagaggt ggcgggggca gtgggggagg gggaagtgga ggcggcggtt ctggcggggg
     2761 aggctccggt ggcgggggga gcgggggtgg gagtaagctt gtgagcaagg gcgaggagct
     2821 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2881 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttgat
     2941 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg
     3001 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3061 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3121 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3181 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3241 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcaa acttcaagat
     3301 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     3361 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact
     3421 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3481 cgggatcact ctcggcatgg acgagctgta caagtgaata cgtatgtgta attatgtgaa
     3541 cgcgtaccgg tcctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt
     3601 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3661 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3721 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3781 tcaatgtatc ttatcatgtc tggatcaatt cgtaatcatg gtcatagctg tttcctgtgt
     3841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4621 cagagttctt gaagtggtgg cctaactacg gctacagtag aaggacagta tttggtatct
     4681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5941 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6001 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     6061 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6121 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggtt
     6181 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6241 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     6301 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6361 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6421 gtaaaacgac ggccagtgcc aagttgggat ctttgcattg gcccacggct ctcaggatgg
     6481 ggatgctccc cttcagcacc cggttcccct tggaaactga tggtcctggc tctgtggcat
     6541 ggcagtggca ctgtgaggag cccctaccag cagcacacag tgggtttggc actgccacgc
     6601 tccggatgcc gcgctctgat ccaaccccat aatcaaggga acccgaattg ccccatcatt
     6661 gcccccacca cccccatcct gccgggccct cacaccccac gctgccttgt ggtgacattc
     6721 cccagcccaa acccacggct tcatggctac cgcggggcat ttcccattgc cgccccatta
     6781 tcagctctgc acacctcccg ctgtacccat gcctcgtggc tgcccttctt tgacgtataa
     6841 tcttctaatt aatacccggc cttgtcaaag tggagcacaa acgttaatta attccccagc
     6901 aggcaggtaa ttaacagtgt gactcccttt ttgctgcgag tggggctgat acagagagat
     6961 gtggcactat ggagcccacg gggtcctggc actgggtgcc cacggaggtc cccatgtgct
     7021 gcagtgtcac cgcctccgag gtgacagtat tgtccctgcg gtgtccctgc agctcagctc
     7081 tgtccacagg gccacctcca gtttggaggg gacacaatgc agccccgatg caacccatcc
     7141 tcgcagcatc ccagggacaa agaccccact gcaagaccgc acacagggct gggtcccgct
     7201 cccctaatat ctacagtgct tttgcatggc cccttaatca atgcagttaa tcagcatgcg
     7261 ctcatgcacc gctctggagc tgcaaagccc ctcgcagcgc tgctcaccaa caccgcgcac
     7321 cgccccggcc cagcctgcag cacgcgctgc aaacaggaaa gaaacaaaat attgcccaaa
     7381 tgtaggcaaa ggcattcggc tgccttgacc tccgccgggc cgggccctgc ctgactcagc
     7441 tccttactca gcgctcgctt cctccctccg gctgccaccg ccgcagcgca caccctgaca
     7501 aagagtggcc cttaacgggc tctgaggtgc acccagcagt gcactcagca gtccaagggc
     7561 cggcctggag gtttgcaccg ctacgtgctg acattagcat tgaacttggc cctgggtagt
     7621 gctgcaggcc gggcggggtg ggtgtagaga gtgcagcgcg cgttgcaccc ggtgcccctt
     7681 cccctccctt gcatcccagc aggctgcacc ccagcaccag gcccgtgcat gcatgctcct
     7741 ggtgttattg cagcctggtg catgcatgcg tcttagtggt gcagcgctgt gcatgcatcc
     7801 tccttggtgt gtagcagctt agtgcatgca tacccctcgg tgttattgct gctctgtgca
     7861 ggcacgctca ttgtatcact tcatcccagt gcatgcactc acactggagc gattgctgct
     7921 cggtgcacgc acactcattg tatcacgtca gctcagtggc tgcacgcaca ccggtgttat
     7981 tgctgctcgg tgcgtgcatg cacatcagtg tcgctgcagc tcagtgcatg cacgctcatt
     8041 gcccatcgct atccctgcct ctcctgctgg cgctccccgg gaggtgactt caaggggacc
     8101 gcaggaccac ctcgggggtg gggggagggc tgcacacgcg gaccccgctc cccctcccca
     8161 acaaagcact gtggaatcaa aaagggggga ggggggatgg aggggcgcgt cacacccccg
     8221 ccccacaccc tcacctcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     8281 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     8341 cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     8401 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     8461 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggg
//
