LOCUS       pLV-mCherry-hCdt1(1-100 8532 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..1172
                     /label="EF-1a"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1149..1173
                     /label="pr092_EF1a_for"
                     /note="sequence: tttcttccatttcaggtgtcgtgag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1182..1889
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1182..2219
                     /label="Translation 1182-2219"
     misc_feature    1890..2213
                     /label="codon optimized"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1890..2219
                     /label="cdt1 1-100 del cy"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2194..2219)
                     /label="pr105_CRL4_xmaI_rev"
                     /note="sequence: attgttccagacgcgccccgcccgggttaTTTCTTTATTTTCTGTCCCGGAGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2220..2222
                     /label="Translation 2220-2222"
     misc_feature    complement(3782..3801)
                     /label="SV40polyA-2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3800..3820)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3804..3820)
                     /label="attP2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3805..3825)
                     /label="M13Rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3806..3825
                     /label="Primer1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3818..3837)
                     /label="oQE31-III"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_binding    complement(3826..3848)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(3845..3862)
                     /label="P65"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3852..3869)
                     /label="P119H3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3878..3895)
                     /label="LacZRev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4041..4058)
                     /label="PT3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4173..4192)
                     /label="TempTest1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4265..4282
                     /label="f-xa.7"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4267..4284)
                     /label="f-xa.J"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4430..4449
                     /label="TempTest2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4711..4728
                     /label="f-xa.9"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(4965..5624)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5173..5190
                     /label="f-xa.11"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5179..5196)
                     /label="f-xa.E"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5619..5636
                     /label="f-xa.13"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5622..5639)
                     /label="f-xa.C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5798..5815
                     /label="pRLTK.R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5936..5953
                     /label="pQEVecPromRegion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5977..5994)
                     /label="SG53"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6055..6073
                     /label="pCEPF"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6055..6073
                     /label="pCEPF"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6066..6085
                     /label="EBVRev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
       61 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      121 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      181 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      241 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      301 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      361 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      421 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      481 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      541 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      601 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      661 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
      721 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
      781 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
      841 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
      901 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
      961 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1021 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1081 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1141 aaagtttttt tcttccattt caggtgtcgt gaggatccAC Catggtgagc aagggcgagg
     1201 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1261 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1321 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1381 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1441 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1501 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1561 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1621 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1681 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1741 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1801 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1861 ccaccggcgg catggacgag ctgtacaagG GATATCCGAG TCATTGGCGA CCGCTCGAGA
     1921 TGGAACAACG GAGGGTTACT GACTTTTTCG CGAGGAGACG ACCCGGCCCC CCGAGGATCG
     1981 CACCCCCGAA ACTCGCTTGT AGAACCCCAT CTCCCGCCAG GCCAGCGCTT CGGGCGCCAG
     2041 CAAGTGCTAC TTCTGGTAGT CGAAAACGGG CGAGACCACC TGCTGCTCCG GGGAGAGACC
     2101 AGGCAAGACC CCCGGCGAGG GCAGCTGCCA GATTGAGTGT TGACGAAGTA TCCTCTCCAT
     2161 CTACTCCAGA AGCACCTGAC ATTCCGGCTT GCCCCTCTCC GGGACAGAAA ATAAAGAAAt
     2221 aacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     2281 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2341 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2401 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2461 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2521 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2581 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2641 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2701 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2761 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2821 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     2881 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     2941 gaggaggtgg gtttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     3001 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     3061 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     3121 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     3181 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     3241 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     3301 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     3361 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     3421 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     3481 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3661 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3721 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct
     3781 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5941 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
     6001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     6121 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     6181 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     6241 attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta
     6301 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     6361 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     6421 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     6481 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     6541 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     6601 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     6661 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     6721 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     6781 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6841 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6901 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6961 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7021 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7081 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7141 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7201 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7261 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7321 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7381 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7441 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     7501 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7561 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7621 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7681 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7741 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7801 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7861 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7921 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7981 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8041 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8101 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8161 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8221 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     8281 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     8341 acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     8401 tagacataat agcaacagac atacaaacta aagaactaca aaaacaaatt acaaaaattc
     8461 aaaattttcg ggtttattac agggacagca gagatccagt ttatcgatga ggccctttcg
     8521 tcttcactcg ag
//