LOCUS       pLV-TetOn-ND-Cdt1-mCher 8902 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            pLV-TRE-NLS-mRuby3.gbk lentiviral backbone. Source name:
            pLV-TRE-NLS-mRuby3 (Note: Geneious sometimes stores the sequence
            name in the source attribute)
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1303..1536
                     /label="RRE"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2028..2143
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2151..2208
                     /label="TRE-For (first round 54.1C)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        2203..2220
                     /label="TetOn element"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2238..2255
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2274..2291
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2310..2327
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2345..2362
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2381..2398
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2441..2459
                     /label="VectorPrimer3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2442..2462
                     /label="pCEP4Fwd"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2483..2501
                     /label="pr113_pCW_Cdt1ND_for"
                     /note="sequence: cagatcgcctggagaattggctagcgccaccATGATAGCTCCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2490..2512
                     /label="pr091_TRE_Eco_Cdt1delPIPmutCy"
                     /note="sequence: agtgaaccgtcagatcgcaccggcGAATTCgccaccATGATAGCTCCGCCAAAGCTCGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2490..4073
                     /label="CDT1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2490..4844
                     /label="Translation 2490-4844"
     misc_feature    2493..2672
                     /label="codon optimized"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2637..2645
                     /label="Cy motif"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4054..4074)
                     /label="pr053_Cdt1_linker_rev"
                     /note="sequence: ggatccCCCAGCGCTTCCTGCACCCAGCCCCTCCTCAGCACGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4074..4097
                     /label="linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4104..4124
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4118..4136)
                     /label="pr115_NLS_HApCW_rev"
                     /note="sequence: gcaaccccaaccccggatcGAATTCtcatgcgtagtctggtacgtcgtacggataaCCACTtccgCCcactttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4136..4154
                     /label="pr077_linker_NLS_mCherry_for"
                     /note="sequence: GTGCAGGAAGCGCTGGGggatccatggcacctaagaaaaagcggaaagtgGGcggaAGTGGtatggtgagcaagggcgag"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4137..4844
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4824..4847)
                     /label="pr076_mCherry_WPRE_rev"
                     /note="sequence: attgttccagacgcgccccgcccgggtcacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4826..4847)
                     /label="pr112_mCherry_pCW_rev"
                     /note="sequence: gcaaccccaaccccggatcGAATTCtcacttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             4845..4847
                     /label="Translation 4845-4847"
     misc_feature    4871..5459
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4946..4963)
                     /label="pWPTRev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(5342..5353)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     LTR             5666..6299
                     /label="3' LTR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(6405..6424)
                     /label="SV40polyA-2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6427..6443)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(6427..6443)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(6427..6443)
                     /label="attP2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6428..6448)
                     /label="M13Rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6429..6448
                     /label="Primer1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6440..6462)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(6441..6460)
                     /label="oQE31-III"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    6451..6467
                     /label="lac operator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(6468..6485)
                     /label="P65"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6475..6492)
                     /label="P119H3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        complement(6475..6505)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(6501..6518)
                     /label="LacZRev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    6520..6541
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(6658..6675)
                     /label="L4440"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(6664..6681)
                     /label="PT3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6796..6815)
                     /label="TempTest1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      complement(6829..7417)
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6888..6905
                     /label="f-xa.7"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6890..6907)
                     /label="f-xa.J"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6909..6928)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7053..7072
                     /label="TempTest2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    7334..7351
                     /label="f-xa.9"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(7356..7373)
                     /label="f-xa.G"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(7588..8448)
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7799..7816
                     /label="f-xa.11"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(7805..7822)
                     /label="f-xa.E"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     8211..8230
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    8245..8262
                     /label="f-xa.13"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(8248..8265)
                     /label="f-xa.C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    8424..8441
                     /label="pRLTK.R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        complement(8449..8553)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    8562..8579
                     /label="pQEVecPromRegion"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    8601..8735
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(8603..8620)
                     /label="SG53"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(8638..8657)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    8681..8699
                     /label="pCEPF"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     8692..8711
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    8692..8711
                     /label="EBVRev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatagacta gacgagttta ctccctatca gtgatagaga
     2221 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2281 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2341 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2401 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg caccggcGAA TTCgccaccA TGATAGCTCC GCCAAAGCTC GCTTGTCGGA
     2521 CTCCCTCTCC GGCTAGACCG GCACTTCGCG CCCCCGCCTC CGCTACTTCA GGTAGCCGAA
     2581 AGAGGGCGAG GCCACCAGCA GCCCCCGGAC GCGATCAGGC GAGGCCACCT GCTCGAGCGG
     2641 CTGCTCGCCT CTCAGTAGAT GAGGTTAGCA GCCCCAGTAC CCCCGAGGCC CCAGACATCC
     2701 CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAATCCAC CCCGGCAGCA GGTCAGCCGC
     2761 CCCACCTGAC ATCCGCGCAG GACCAGGACA CCATCTCTGA GCTTGCGTCA TGCCTGCAAC
     2821 GGGCCCGGGA GCTGGGGGCA AGAGTCCGGG CGCTGAAGGC CAGTGCCCAG GATGCTGGGG
     2881 AGTCCTGCAC CCCAGAGGCC GAGGGCCGCC CTGAGGAGCC ATGTGGCGAG AAGGCGCCCG
     2941 CCTACCAGCG CTTCCATGCC CTGGCCCAGC CCGGCCTGCC GGGACTCGTG CTGCCCTACA
     3001 AGTACCAGGT GCTGGCGGAG ATGTTCCGCA GCATGGACAC CATCGTGGGC ATGCTCCACA
     3061 ACCGCTCCGA GACGCCCACC TTTGCCAAGG TCCAGCGGGG CGTCCAGGAC ATGATGCGTA
     3121 GGCGTTTTGA GGAGCGCAAT GTTGGCCAGA TCAAAACCGT GTACCCGGCC TCCTACCGCT
     3181 TCCGCCAGGA GCGCAGTGTC CCCACCTTCA AGGATGGCAC CAGGAGGTCA GATTACCAGC
     3241 TCACCATCGA GCCACTGCTG GAGCAGGAGG CTGACGGAGC AGCCCCCCAG CTCACGGCCT
     3301 CGCGCCTCCT GCAGCGACGG CAGATCTTCA GCCAGAAGCT GGTGGAGCAT GTCAAGGAGC
     3361 ACCACAAGGC CTTCCTGGCC TCCCTGAGCC CCGCCATGGT GGTGCCGGAG GACCAGCTGA
     3421 CCCGCTGGCA CCCGCGCTTC AACGTGGATG AAGTACCCGA CATCGAGCCG GCCGCGCTGC
     3481 CCCAGCCACC CGCCACGGAG AAGCTCACCA CTGCTCAGGA GGTGCTGGCC CGGGCCCGCA
     3541 ACCTGATTTC ACCCAGGATG GAGAAGGCCT TGAGTCAATT GGCCCTGCGC TCTGCTGCGC
     3601 CCAGCAGCCC CGGGTCTCCC AGGCCAGCAC TGCCGGCTAC CCCACCAGCC ACCCCGCCTG
     3661 CAGCCTCTCC CAGTGCTCTG AAGGGGGTGT CCCAGGATCT GCTGGAGCGG ATCCGAGCCA
     3721 AGGAGGCACA GAAGCAGCTG GCACAGATGA CGCGGTGCCC GGAGCAGGAG CAGCGGCTGC
     3781 AGCGCTTAGA ACGGCTGCCT GAGCTGGCCC GCGTGCTGCG GAGCGTCTTT GTGTCCGAAC
     3841 GCAAGCCTGC GCTCAGCATG GAGGTGGCCT GTGCCAGGAT GGTGGGCAGC TGTTGTACTA
     3901 TCATGAGCCC TGGGGAAATG GAGAAGCACC TGCTGCTCCT CTCCGAGCTG CTGCCGGACT
     3961 GGCTCAGCCT CCACCGCATC CGCACCGACA CCTACGTCAA GCTGGACAAG GCCGCGGACC
     4021 TCGCCCACAT CACTGCACGC CTGGCCCACC AGACACGTGC TGAGGAGGGG CTGGGTGCAG
     4081 GAAGCGCTGG Gggatccatg gcacctaaga aaaagcggaa agtgGGcgga AGTGGtatgg
     4141 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     4201 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4261 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     4321 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     4381 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4441 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     4501 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     4561 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     4621 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     4681 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     4741 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     4801 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaccc gggcggggcg
     4861 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5281 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5461 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     5521 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     5581 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5641 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     5701 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     5761 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5821 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5881 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5941 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     6001 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     6061 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6301 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6361 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     6421 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6541 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6601 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7261 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7561 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7621 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7681 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7741 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7801 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7861 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7921 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7981 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8041 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8101 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8161 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8221 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8281 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8341 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8401 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8461 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8521 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8581 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8701 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     8761 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     8821 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     8881 tatgtactac aaacttagta gt
//