LOCUS       SpCas9_TadCBEe          8778 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
ACCESSION   urn.local...f-edm9ixv
COMMENT     Imported using the Genbank importer. File name: 3 documents from
            AtoC_mammalianCells.gb
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label="CMV promoter, full"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer_bind     complement(51..70)
                     /label="QB1110"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: TTATGTAACGCGGAACTCCA %GC: 45.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB1110 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1sh-c6uht7h"""">QB1110</a>"
     Promoter        508..589
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="ApEinfo_revcolor: #b4abac"
     misc_feature    610..628
                     /label="core promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="ApEinfo_revcolor: #9eafd2"
     primer_bind     610..628
                     /label="T7Fshort"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: TAATACGACTCACTATAGG %GC: 36.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: T7Fshort Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1uf-c6uhujr"""">T7Fshort</a>"
     primer_bind     610..629
                     /label="QB0106"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: TAATACGACTCACTATAGGG %GC: 40.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0106 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ov-c6uht6x"""">QB0106</a>"
     promoter        610..632
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    618..620
                     /label="essential bases"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    622..622
                     /label="essential base"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    627..627
                     /label="TSS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="ApEinfo_revcolor: #75c6a9"
     misc_feature    630..632
                     /label="modest consensus on RRR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     NLS             642..698
                     /label="Bipartite SV40 NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     CDS             642..6020
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    702..1199
                     /label="TadA-CDe"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             702..1199
                     /label="Translation 702-1199"
     CDS             777..782
                     /label="Translation 777-782"
     CDS             879..881
                     /label="Translation 879-881"
     CDS             1200..1295
                     /label="BE4 linker Genscript codons"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     CDS             1200..1295
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1296..5396
                     /label="nCas9, Genscript codons"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     CDS             1296..5396
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1320..1322
                     /label="D10A"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     primer_bind     4958..4977
                     /label="oSP244"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: GGGAAACGAACTGGCCCTGC %GC: 65.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP244 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:3dv-c89qlzm"""">oSP244</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1fn-cfz7eso"""">oSP244</a>"
     source          5397..5966
                     /label="2xUGI Genscript codons"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #b7e6d7"
     NLS             5967..5996
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer_bind     complement(5986..6022)
                     /label="oSP223"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Sequence: GGTTAGACTTTCCTCTTCTTCTTGGGCTCGAATTCGC %GC: 48.6 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP223 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2r4-cf0ayke"""">oSP223</a>"
     misc_feature    5997..6017
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     primer_bind     complement(6022..6047)
                     /label="oSP294"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: CTCAATGGTGATGGTGATGATGACCG %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP294 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:30z-cf0ayl2"""">oSP294</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ml-cfz7et9"""">oSP294</a>"
     primer_bind     complement(6062..6084)
                     /label="oSP206"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Sequence: CTAGAAGGCACAGTCGAGGCTGA %GC: 56.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP206 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1xh-c6ui0ih"""">oSP206</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2or-cf0ayk4"""">oSP206</a>"
     primer_bind     complement(6066..6083)
                     /label="QB0008"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: TAGAAGGCACAGTCGAGG %GC: 55.6 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0008 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1b4-c6uht4u"""">QB0008</a>"
     Terminator      6069..6296
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #c7b0e3"
     primer_bind     complement(6367..6383)
                     /label="QB0048"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: CAGGAAACAGCTATGAC %GC: 47.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0048 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1go-c6uht5t"""">QB0048</a>"
     primer_bind     complement(6380..6402)
                     /label="QB0050"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Sequence: AGCGGATAACAATTTCACACAGG %GC: 43.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0050 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1gy-c6uht5u"""">QB0050</a>"
     primer_bind     complement(6380..6403)
                     /label="QB0108"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: GAGCGGATAACAATTTCACACAGG %GC: 45.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0108 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1p5-c6uht6y"""">QB0108</a>"
     Promoter        complement(6416..6445)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    6562..6562
                     /label="a"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6585..6591
                     /label="removed SapI site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
     primer_bind     complement(6597..6615)
                     /label="QB0039"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: AGCGAGTCAGTGAGCGAGG %GC: 63.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0039 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ff-c6uht5n"""">QB0039</a>"
     primer_bind     complement(6783..6800)
                     /label="oSP301"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Sequence: GATGCTCGTCAGGGGGGC %GC: 72.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP301 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:32i-cf0ayl5"""">oSP301</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2i2-cf082c6"""">oSP301</a>"
     primer_bind     complement(6870..6887)
                     /label="Ju1-R2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Sequence: GCACGAGGGAGCTTCCAG %GC: 66.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R2 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1v9-c6uhujv"""">Ju1-R2</a>"
     primer_bind     6992..7010
                     /label="QB0766"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: GTGTAGGTCGTTCGCTCCA %GC: 57.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0766 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1rs-c6uht7c"""">QB0766</a>"
     primer_bind     complement(7117..7136)
                     /label="QB0767"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: TCCTGTTACCAGTGGCTGCT %GC: 55.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0767 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1rx-c6uht7d"""">QB0767</a>"
     misc_feature    7391..7391
                     /label="G>A"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    7421..7431
                     /label="USER jct 25"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="ApEinfo_revcolor: #f8d3a9"
     Marker          complement(7528..8388)
                     /label="bla beta lactamase (ampicillin/carbenicillinresistance)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     primer_bind     complement(7574..7594)
                     /label="Ju1-R1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Sequence: GGCAACTATGGATGAACGAAA %GC: 42.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R1 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1v4-c6uhuju"""">Ju1-R1</a>"
     misc_feature    7668..7673
                     /label="removed BsaI site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #b7e6d7"
     misc_feature    7671..7679
                     /label="USER jct 31"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="ApEinfo_revcolor: #9eafd2"
     primer_bind     8151..8170
                     /label="QB0005"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: ATAATACCGCGCCACATAGC %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0005 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ap-c6uht4m"""">QB0005</a>"
     Promoter        complement(8430..8458)
                     /label="amp promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
     primer_bind     8502..8519
                     /label="QB0090"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: CCCGAAAAGTGCCACCTG %GC: 61.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0090 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1mn-c6uht6m"""">QB0090</a>"
     primer_bind     complement(8572..8591)
                     /label="QB0092"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: CGGCATCAGAGCAGATTGTA %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0092 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1mx-c6uht6n"""">QB0092</a>"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc
      601 cgcggccgct aatacgactc actataggga gagccgccac catgaaacgg acagccgacg
      661 gaagcgagtt cgagtcacca aagaagaagc ggaaagtcag tTCTGAGGTG GAGTTTTCCC
      721 ACGAGTACTG GATGAGACAT GCCCTGACCC TGGCCAAGAG GGCACGGGAT GAGAGGGCGG
      781 GGCCTGTGGG AGCCGTGCTG GTGCTGAACA ATAGAGTGAT CGGCGAGGGC TGGAACAGAG
      841 CCATCGGCCT GCACGACCCA ACAGCCCATG CCGAAATTAT GGCCCTGAGA CAGGGCGGCC
      901 TGGTCATGCA GAACCACAGA CTGATTGACG CCACCCTGTA CGTGACATTC GAGCCTTGCG
      961 TGATGTGCGC CGGCGCCATG ATCAACTCTA GGATCGGCCG CGTGGTGTTT GGCGTGAGGA
     1021 ACTCAAAAAG AGGCGCCGCA GGCTCCCTAA TGAACGTGCT GAACTACCCC GGCATGAATC
     1081 ACCGCGTCGA AATTACCGAG GGAATCCTGG CAGATGAATG TGCCGCCCTG CTGTGCGATT
     1141 TCTATCGGAT GCCTAGACAT GTGTTCAATT CTCAGAAGAA GGCCCAGAGC TCCATCAACt
     1201 ctggcggatc tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa
     1261 caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc atcggcctgg
     1321 ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     1381 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     1441 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     1501 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     1561 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     1621 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     1681 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     1741 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     1801 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     1861 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     1921 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     1981 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     2041 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     2101 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     2161 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     2221 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     2281 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     2341 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     2401 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     2461 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     2521 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     2581 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     2641 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     2701 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     2761 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     2821 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     2881 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     2941 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     3001 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     3061 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     3121 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     3181 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     3241 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     3301 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     3361 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     3421 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     3481 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     3541 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     3601 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     3661 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     3721 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     3781 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     3841 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     3901 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     3961 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     4021 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     4081 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     4141 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     4201 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     4261 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     4321 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     4381 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     4441 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     4501 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     4561 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     4621 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     4681 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     4741 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     4801 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     4861 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     4921 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     4981 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     5041 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     5101 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     5161 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     5221 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     5281 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     5341 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgacagcg
     5401 gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag aaggagactg
     5461 ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga
     5521 tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg
     5581 agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc
     5641 aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga ggatctggag
     5701 gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg gtcatccagg
     5761 agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag cctgagagcg
     5821 atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg ctgctgacat
     5881 ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac ggagagaaca
     5941 aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca
     6001 agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg
     6061 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6121 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6181 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6241 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6301 tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta
     6361 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6421 acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt
     6481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgcactt ccgcttcctc
     6601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7381 ggggtctgac actcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc
     7681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8521 cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg
     8581 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8641 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     8701 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     8761 ttgacattga ttattgac
//