LOCUS       SaCas9_TadCBEa          7836 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
ACCESSION   urn.local...f-edm9ixv
COMMENT     Imported using the Genbank importer. File name: 3 documents from
            AtoC_mammalianCells.gb
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label="CMV promoter, full"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer_bind     complement(51..70)
                     /label="QB1110"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: TTATGTAACGCGGAACTCCA %GC: 45.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB1110 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1sh-c6uht7h"""">QB1110</a>"
     Promoter        508..589
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="ApEinfo_revcolor: #b4abac"
     primer_bind     519..539
                     /label="QB0012"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: CGCAAATGGGCGGTAGGCGTG %GC: 66.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0012 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1bo-c6uht51"""">QB0012</a>"
     primer_bind     526..547
                     /label="QB0976"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Sequence: GGGCGGTAGGCGTGTACGGTGG %GC: 72.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0976 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1s2-c6uht7e"""">QB0976</a>"
     primer_bind     610..628
                     /label="T7Fshort"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: TAATACGACTCACTATAGG %GC: 36.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: T7Fshort Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1uf-c6uhujr"""">T7Fshort</a>"
     misc_feature    610..628
                     /label="core promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="ApEinfo_revcolor: #9eafd2"
     primer_bind     610..629
                     /label="QB0106"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: TAATACGACTCACTATAGGG %GC: 40.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0106 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ov-c6uht6x"""">QB0106</a>"
     promoter        610..632
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
     misc_feature    618..620
                     /label="essential bases"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    622..622
                     /label="essential base"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    627..627
                     /label="TSS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="ApEinfo_revcolor: #75c6a9"
     misc_feature    630..632
                     /label="modest consensus on RRR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
     NLS             642..698
                     /label="Bipartite SV40 NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     CDS             642..5078
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    674..698
                     /label=1609
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    702..1199
                     /label="TadA-CDa"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1200..1295
                     /label="BE4 linker Genscript codons"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    1296..4454
                     /label="SaCas9 D10A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1344..1368)
                     /label="MN1826"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1787..1809
                     /label="MN35"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3158..3180
                     /label="37 seq"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3283..3311)
                     /label=64
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3301..3328
                     /label="68SOE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3312..3314
                     /label="G to A at wobble to remove SapI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3315..3320
                     /label="SapI_recognition"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3315..3336
                     /label=68
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3857..3885
                     /label="38 seq"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4424..4454)
                     /label=65
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     source          4455..5024
                     /label="2xUGI Genscript codons"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #b7e6d7"
     primer_bind     5016..5036
                     /label="oSP256"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: GGCGGCTCAAAAAGAACCGCC %GC: 61.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP256 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1hb-cfz7esv"""">oSP256</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2vp-cf0aykr"""">oSP256</a>"
     primer_bind     5020..5042
                     /label="oSP295"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: GCTCAAAAAGAACCGCCGACGGC %GC: 60.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP295 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:314-cf0ayl3"""">oSP295</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:a6l0-cdktb0b"""">oSP295</a>"
     NLS             5025..5054
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
     primer_bind     complement(5044..5080)
                     /label="oSP223"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Sequence: GGTTAGACTTTCCTCTTCTTCTTGGGCTCGAATTCGC %GC: 48.6 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP223 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2r4-cf0ayke"""">oSP223</a>"
     primer_bind     complement(5045..5080)
                     /label="oSP238"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Sequence: GGTTAGACTTTCCTCTTCTTCTTGGGCTCGAATTCG %GC: 47.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP238 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2t7-cf0aykm"""">oSP238</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1et-cfz7esh"""">oSP238</a>"
     primer_bind     complement(5046..5080)
                     /label="oSP253"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Sequence: GGTTAGACTTTCCTCTTCTTCTTGGGCTCGAATTC %GC: 45.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP253 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2va-cf0aykr"""">oSP253</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1gw-cfz7est"""">oSP253</a>"
     misc_feature    5055..5075
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     primer_bind     5060..5097
                     /label="oSP237"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Sequence: GAAGAAGAGGAAAGTCTAACCGGTCATCATCACCATCA %GC: 44.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP237 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1eo-cfz7esf"""">oSP237</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2t2-cf0aykl"""">oSP237</a>"
     primer_bind     complement(5080..5105)
                     /label="oSP294"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: CTCAATGGTGATGGTGATGATGACCG %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP294 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:30z-cf0ayl2"""">oSP294</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ml-cfz7et9"""">oSP294</a>"
     primer_bind     complement(5120..5142)
                     /label="oSP206"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Sequence: CTAGAAGGCACAGTCGAGGCTGA %GC: 56.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP206 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1xh-c6ui0ih"""">oSP206</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2or-cf0ayk4"""">oSP206</a>"
     primer_bind     complement(5124..5141)
                     /label="QB0008"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: TAGAAGGCACAGTCGAGG %GC: 55.6 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0008 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1b4-c6uht4u"""">QB0008</a>"
     Terminator      5127..5354
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #c7b0e3"
     primer_bind     complement(5425..5441)
                     /label="QB0048"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: CAGGAAACAGCTATGAC %GC: 47.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0048 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1go-c6uht5t"""">QB0048</a>"
     primer_bind     complement(5438..5460)
                     /label="QB0050"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Sequence: AGCGGATAACAATTTCACACAGG %GC: 43.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0050 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1gy-c6uht5u"""">QB0050</a>"
     primer_bind     complement(5438..5461)
                     /label="QB0108"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: GAGCGGATAACAATTTCACACAGG %GC: 45.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0108 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1p5-c6uht6y"""">QB0108</a>"
     Promoter        complement(5474..5503)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    5620..5620
                     /label="G>A in Addgene deposit vs. original map"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
     misc_feature    5643..5649
                     /label="removed SapI site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
     misc_feature    5645..5653
                     /label="USER jct 26"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
     primer_bind     complement(5655..5673)
                     /label="QB0039"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Sequence: AGCGAGTCAGTGAGCGAGG %GC: 63.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0039 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ff-c6uht5n"""">QB0039</a>"
     primer_bind     complement(5841..5858)
                     /label="oSP301"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Sequence: GATGCTCGTCAGGGGGGC %GC: 72.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP301 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:32i-cf0ayl5"""">oSP301</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2i2-cf082c6"""">oSP301</a>"
     primer_bind     complement(5928..5945)
                     /label="Ju1-R2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Sequence: GCACGAGGGAGCTTCCAG %GC: 66.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R2 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1v9-c6uhujv"""">Ju1-R2</a>"
     primer_bind     6050..6068
                     /label="QB0766"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: GTGTAGGTCGTTCGCTCCA %GC: 57.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0766 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1rs-c6uht7c"""">QB0766</a>"
     primer_bind     complement(6175..6194)
                     /label="QB0767"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: TCCTGTTACCAGTGGCTGCT %GC: 55.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0767 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1rx-c6uht7d"""">QB0767</a>"
     misc_feature    6419..6419
                     /label="G>A in Addgene deposit vs. original working copy"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="ApEinfo_revcolor: #75c6a9"
     misc_feature    6449..6449
                     /label="G>A"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #d59687"
     misc_feature    6479..6489
                     /label="USER jct 25"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="ApEinfo_revcolor: #f8d3a9"
     Marker          complement(6586..7446)
                     /label="bla beta lactamase (ampicillin/carbenicillinresistance)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     primer_bind     complement(6632..6652)
                     /label="Ju1-R1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Sequence: GGCAACTATGGATGAACGAAA %GC: 42.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R1 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1v4-c6uhuju"""">Ju1-R1</a>"
     misc_feature    6726..6731
                     /label="removed BsaI site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #b7e6d7"
     misc_feature    6729..6737
                     /label="USER jct 31"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="ApEinfo_revcolor: #9eafd2"
     primer_bind     complement(7062..7085)
                     /label="oSP257"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Sequence: GCAGTGCTGCCATAACCATGAGTG %GC: 54.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP257 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1hg-cfz7esv"""">oSP257</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2vu-cf0ayks"""">oSP257</a>"
     primer_bind     7209..7228
                     /label="QB0005"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: ATAATACCGCGCCACATAGC %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0005 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1ap-c6uht4m"""">QB0005</a>"
     Promoter        complement(7488..7516)
                     /label="amp promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
     primer_bind     7501..7527
                     /label="oSP302"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Sequence: CGGATACATATTTGAATGTATTTAGAA %GC: 25.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP302 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:32n-cf0ayl5"""">oSP302</a> Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:2i7-cf082c8"""">oSP302</a>"
     primer_bind     7554..7577
                     /label="oSP99"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Sequence: CATTTCCCCGAAAAGTGCCACCTG %GC: 54.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP99 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1x7-c6uhy1r"""">oSP99</a>"
     primer_bind     7560..7577
                     /label="QB0090"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: CCCGAAAAGTGCCACCTG %GC: 61.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0090 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1mn-c6uht6m"""">QB0090</a>"
     primer_bind     complement(7630..7649)
                     /label="QB0092"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: CGGCATCAGAGCAGATTGTA %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0092 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; <ahref=""""urn:local:.:1mx-c6uht6n"""">QB0092</a>"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc
      601 cgcggccgct aatacgactc actataggga gagccgccac catgaaacgg acagccgacg
      661 gaagcgagtt cgagtcacca aagaagaagc ggaaagtcag tTCTGAGGTG GAGTTTTCCC
      721 ACGAGTACTG GATGAGACAT GCCCTGACCC TGGCCAAGAG GGCACGGGAT GAGGGGGCGG
      781 GTCCTGTGGG AGCCGTGCTG GTGCTGAACA ATAGAGTGAT CGGCGAGGGC TGGAACAGAG
      841 CCATCGGCCT GCACGACCCA ACAGCCCATG CCGAAATTAT GGCCCTGAGA CAGGGCGGCC
      901 TGGTCATGCA GAACTACAGA CTGTTTGACG CCACCCTGTA CGTGACATTC GAGCCTTGCG
      961 TGATGTGCGC CGGCGCCATG ATCAACTCTA GGATCGGCCG CGTGGTGTTT GGCGTGAGGA
     1021 ACTCAAAAAG AGGCGCCGCA GGCTCCCTGA TGAACGTGCT GAACTACCCC GGCATGAATC
     1081 ACCGCGTCGA AATTACCGAG GGAATCCTGG CAGATGAATG TGCCGCCCTG CTGTGCGATT
     1141 TCTATCGGAT GCCTAGACAG GTGTTCAATT CTCAGAAGAA GGCCCAGAGC TCCATCAACt
     1201 ctggcggatc tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa
     1261 caccagagag cagtggcggc agcagcggcg gcagcgggaa gcgaaattac attctggggc
     1321 tggccattgg cattacatca gtgggctatg gcatcattga ctacgagaca agggacgtga
     1381 tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag ggccggagat
     1441 ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag agagtgaaga
     1501 agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc atcaatcctt
     1561 atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt agcgccgccc
     1621 tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag gaggacaccg
     1681 gcaacgagct gtccacaaag gagcagatca gccgcaattc caaggccctg gaggagaagt
     1741 atgtggccga gctgcagctg gagcggctga agaaggatgg cgaggtgagg ggctccatca
     1801 atcgcttcaa gacctctgac tacgtgaagg aggccaagca gctgctgaag gtgcagaagg
     1861 cctaccacca gctggatcag tcctttatcg atacatatat cgacctgctg gagacaaggc
     1921 gcacatacta tgagggacca ggagagggct ctcccttcgg ctggaaggac atcaaggagt
     1981 ggtacgagat gctgatgggc cactgcacct attttccaga ggagctgaga agcgtgaagt
     2041 acgcctataa cgccgatctg tacaacgccc tgaatgacct gaacaacctg gtcatcacca
     2101 gggatgagaa cgagaagctg gagtactatg agaagttcca gatcatcgag aacgtgttca
     2161 agcagaagaa gaagcctaca ctgaagcaga tcgccaagga gatcctggtg aacgaggagg
     2221 acatcaaggg ctaccgcgtg acctccacag gcaagccaga gttcaccaat ctgaaggtgt
     2281 atcacgatat caaggacatc acagcccgga aggagatcat cgagaacgcc gagctgctgg
     2341 atcagatcgc caagatcctg accatctatc agagctccga ggacatccag gaggagctga
     2401 ccaacctgaa tagcgagctg acacaggagg agatcgagca gatcagcaat ctgaagggct
     2461 acaccggcac acacaacctg agcctgaagg ccatcaatct gatcctggat gagctgtggc
     2521 acacaaacga caatcagatc gccatcttta accggctgaa gctggtgcca aagaaggtgg
     2581 acctgtccca gcagaaggag atcccaacca cactggtgga cgatttcatc ctgtctcccg
     2641 tggtgaagcg gagcttcatc cagagcatca aagtgatcaa cgccatcatc aagaagtacg
     2701 gcctgcccaa tgatatcatc atcgagctgg ccagggagaa gaactccaag gacgcccaga
     2761 agatgatcaa tgagatgcag aagaggaacc gccagaccaa tgagcggatc gaggagatca
     2821 tcagaaccac aggcaaggag aacgccaagt acctgatcga gaagatcaag ctgcacgata
     2881 tgcaggaggg caagtgtctg tattctctgg aggccatccc tctggaggac ctgctgaaca
     2941 atccattcaa ctacgaggtg gatcacatca tcccccggag cgtgagcttc gacaattctt
     3001 ttaacaataa ggtgctggtg aagcaggagg agaacagcaa gaagggcaat aggacccctt
     3061 tccagtacct gtctagctcc gattctaaga tcagctacga gacattcaag aagcacatcc
     3121 tgaatctggc caagggcaag ggccgcatca gcaagaccaa gaaggagtac ctgctggagg
     3181 agcgggacat caacagattc tccgtgcaga aggacttcat caaccggaat ctggtggaca
     3241 ccagatacgc cacacgcggc ctgatgaatc tgctgcggtc ttatttcaga gtgaacaatc
     3301 tggatgtgaa gGTAaagagc atcaacggcg gcttcacctc ctttctgcgg agaaagtgga
     3361 agtttaagaa ggagcgcaac aagggctata agcaccacgc cgaggatgcc ctgatcatcg
     3421 ccaatgccga cttcatcttt aaggagtgga agaagctgga caaggccaag aaagtgatgg
     3481 agaaccagat gttcgaggag aagcaggccg agagcatgcc cgagatcgag acagagcagg
     3541 agtacaagga gattttcatc acacctcacc agatcaagca catcaaggac ttcaaggact
     3601 acaagtattc tcacagggtg gataagaagc ccaaccgcga gctgatcaat gacaccctgt
     3661 atagcacacg gaaggacgat aagggcaata ccctgatcgt gaacaatctg aacggcctgt
     3721 acgacaagga taatgacaag ctgaagaagc tgatcaacaa gtctcccgag aagctgctga
     3781 tgtaccacca cgatcctcag acatatcaga agctgaagct gatcatggag cagtacggcg
     3841 acgagaagaa cccactgtat aagtactatg aggagacagg caactacctg acaaagtata
     3901 gcaagaagga taatggcccc gtgatcaaga agatcaagta ctatggcaac aagctgaatg
     3961 cccacctgga catcaccgac gattacccta actctcgcaa taaggtggtg aagctgagcc
     4021 tgaagccata ccggttcgac gtgtacctgg acaacggcgt gtataagttt gtgacagtga
     4081 agaatctgga tgtgatcaag aaggagaact actatgaggt gaacagcaag tgctacgagg
     4141 aggccaagaa gctgaagaag atcagcaacc aggccgagtt catcgcctct ttttacaaca
     4201 atgacctgat caagatcaat ggcgagctgt atagagtgat cggcgtgaac aatgatctgc
     4261 tgaacagaat cgaagtgaat atgatcgaca tcacctacag ggagtatctg gagaacatga
     4321 atgataagag gccccctcgc atcatcaaga ccatcgcctc taagacacag agcatcaaga
     4381 agtacagcac agacatcctg gggaacctgt atgaagtcaa gagcaagaaa catcctcaga
     4441 ttatcaagaa aggcagcggc gggagcggcg ggagcggggg gagcactaat ctgagcgaca
     4501 tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg
     4561 aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct
     4621 acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc
     4681 cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag
     4741 gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca
     4801 agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg
     4861 gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa
     4921 atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg
     4981 attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg
     5041 gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca
     5101 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5341 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc
     5401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg
     5521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcggga agaggcggtt tgcgtattgg
     5641 gcgcacttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaaa
     6421 tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat
     6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6721 accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7561 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
     7621 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     7681 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     7741 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     7801 acgggccaga tatacgcgtt gacattgatt attgac
//