LOCUS SaCas9_TadDE 7836 bp ds-DNA circular 25-OCT-2022 DEFINITION . ACCESSION urn.local...f-edm9ixv COMMENT Imported using the Genbank importer. File name: 3 documents from AtoC_mammalianCells.gb FEATURES Location/Qualifiers promoter 1..589 /label="CMV promoter, full" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="ApEinfo_revcolor: #b1ff67" primer_bind complement(51..70) /label="QB1110" /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /note="Sequence: TTATGTAACGCGGAACTCCA %GC: 45.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB1110 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB1110" Promoter 508..589 /label="CMV promoter" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /note="ApEinfo_revcolor: #b4abac" primer_bind 519..539 /label="QB0012" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /note="Sequence: CGCAAATGGGCGGTAGGCGTG %GC: 66.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0012 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0012" primer_bind 526..547 /label="QB0976" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /note="Sequence: GGGCGGTAGGCGTGTACGGTGG %GC: 72.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0976 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0976" misc_feature 610..628 /label="core promoter" /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /note="ApEinfo_revcolor: #9eafd2" primer_bind 610..628 /label="T7Fshort" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /note="Sequence: TAATACGACTCACTATAGG %GC: 36.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: T7Fshort Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; T7Fshort" primer_bind 610..629 /label="QB0106" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /note="Sequence: TAATACGACTCACTATAGGG %GC: 40.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0106 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0106" promoter 610..632 /label="T7 promoter" /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /note="ApEinfo_revcolor: #f58a5e" misc_feature 618..620 /label="essential bases" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /note="ApEinfo_revcolor: #d59687" misc_feature 622..622 /label="essential base" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /note="ApEinfo_revcolor: #d6b295" misc_feature 627..627 /label="TSS" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="ApEinfo_revcolor: #75c6a9" misc_feature 630..632 /label="modest consensus on RRR" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /note="ApEinfo_revcolor: #85dae9" NLS 642..698 /label="Bipartite SV40 NLS" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="ApEinfo_revcolor: #b1ff67" CDS 642..5078 /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" misc_feature 674..698 /label=1609 /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" CDS 699..1199 /label="Translation 699-1199" misc_feature 702..1199 /label="TadDE" /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" CDS 1200..1295 /label="BE4 linker Genscript codons" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /note="ApEinfo_revcolor: #d6b295" CDS 1200..1295 /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" misc_feature 1296..4454 /label="SaCas9 D10A" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" misc_feature 1787..1809 /label="MN35" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" misc_feature 3158..3180 /label="37 seq" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" misc_feature complement(3283..3311) /label=64 /ApEinfo_revcolor="#f8d3a9" /ApEinfo_fwdcolor="#f8d3a9" misc_feature 3301..3328 /label="68SOE" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" misc_feature 3312..3314 /label="G to A at wobble to remove SapI" /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" misc_feature 3315..3320 /label="SapI_recognition" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" misc_feature 3315..3336 /label=68 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" misc_feature 3857..3885 /label="38 seq" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" source 4455..5024 /label="2xUGI Genscript codons" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /note="ApEinfo_revcolor: #b7e6d7" NLS 5025..5054 /label="SV40 NLS" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="ApEinfo_revcolor: #b1ff67" misc_feature 5055..5075 /label="SV40 NLS" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /note="ApEinfo_revcolor: #d59687" primer_bind complement(5080..5105) /label="oSP294" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /note="Sequence: CTCAATGGTGATGGTGATGATGACCG %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP294 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP294 Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; oSP294" primer_bind complement(5120..5142) /label="oSP206" /ApEinfo_revcolor="#f8d3a9" /ApEinfo_fwdcolor="#f8d3a9" /note="Sequence: CTAGAAGGCACAGTCGAGGCTGA %GC: 56.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP206 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP206 Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; oSP206" primer_bind complement(5124..5141) /label="QB0008" /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /note="Sequence: TAGAAGGCACAGTCGAGG %GC: 55.6 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0008 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0008" Terminator 5127..5354 /label="bGH PA terminator" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /note="ApEinfo_revcolor: #c7b0e3" primer_bind complement(5425..5441) /label="QB0048" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /note="Sequence: CAGGAAACAGCTATGAC %GC: 47.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0048 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0048" primer_bind complement(5438..5460) /label="QB0050" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /note="Sequence: AGCGGATAACAATTTCACACAGG %GC: 43.5 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0050 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0050" primer_bind complement(5438..5461) /label="QB0108" /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /note="Sequence: GAGCGGATAACAATTTCACACAGG %GC: 45.8 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0108 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0108" Promoter complement(5474..5503) /label="lac promoter" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /note="ApEinfo_revcolor: #d6b295" misc_feature 5620..5620 /label="G>A in Addgene deposit vs. original map" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /note="ApEinfo_revcolor: #d6b295" misc_feature 5643..5649 /label="removed SapI site" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="ApEinfo_revcolor: #faac61" misc_feature 5645..5653 /label="USER jct 26" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="ApEinfo_revcolor: #faac61" primer_bind complement(5655..5673) /label="QB0039" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /note="Sequence: AGCGAGTCAGTGAGCGAGG %GC: 63.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0039 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0039" primer_bind complement(5841..5858) /label="oSP301" /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" /note="Sequence: GATGCTCGTCAGGGGGGC %GC: 72.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP301 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP301 Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; oSP301" primer_bind complement(5928..5945) /label="Ju1-R2" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /note="Sequence: GCACGAGGGAGCTTCCAG %GC: 66.7 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R2 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; Ju1-R2" primer_bind 6050..6068 /label="QB0766" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /note="Sequence: GTGTAGGTCGTTCGCTCCA %GC: 57.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0766 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0766" primer_bind complement(6175..6194) /label="QB0767" /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /note="Sequence: TCCTGTTACCAGTGGCTGCT %GC: 55.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0767 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0767" misc_feature 6419..6419 /label="G>A in Addgene deposit vs. original working copy" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="ApEinfo_revcolor: #75c6a9" misc_feature 6449..6449 /label="G>A" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /note="ApEinfo_revcolor: #d59687" misc_feature 6479..6489 /label="USER jct 25" /ApEinfo_revcolor="#f8d3a9" /ApEinfo_fwdcolor="#f8d3a9" /note="ApEinfo_revcolor: #f8d3a9" Marker complement(6586..7446) /label="bla beta lactamase (ampicillin/carbenicillinresistance)" /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" /note="ApEinfo_revcolor: #ffef86" primer_bind complement(6632..6652) /label="Ju1-R1" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="Sequence: GGCAACTATGGATGAACGAAA %GC: 42.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: Ju1-R1 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; Ju1-R1" misc_feature 6726..6731 /label="removed BsaI site" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /note="ApEinfo_revcolor: #b7e6d7" misc_feature 6729..6737 /label="USER jct 31" /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /note="ApEinfo_revcolor: #9eafd2" primer_bind complement(7062..7085) /label="oSP257" /ApEinfo_revcolor="#f8d3a9" /ApEinfo_fwdcolor="#f8d3a9" /note="Sequence: GCAGTGCTGCCATAACCATGAGTG %GC: 54.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP257 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP257 Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; oSP257" primer_bind 7209..7228 /label="QB0005" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /note="Sequence: ATAATACCGCGCCACATAGC %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0005 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0005" Promoter complement(7488..7516) /label="amp promoter" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="ApEinfo_revcolor: #faac61" primer_bind 7501..7527 /label="oSP302" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /note="Sequence: CGGATACATATTTGAATGTATTTAGAA %GC: 25.9 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP302 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP302 Alternative_Match: 100.00%; Adjusted-Interval-0:0/0; oSP302" primer_bind 7554..7577 /label="oSP99" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="Sequence: CATTTCCCCGAAAAGTGCCACCTG %GC: 54.2 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: oSP99 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; oSP99" primer_bind 7560..7577 /label="QB0090" /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /note="Sequence: CCCGAAAAGTGCCACCTG %GC: 61.1 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0090 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0090" primer_bind complement(7630..7649) /label="QB0092" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /note="Sequence: CGGCATCAGAGCAGATTGTA %GC: 50.0 note: Derived using Geneious Prime 2020.2.2 'Annotatefrom Database' based on nucleotide similarity Transferred_From: QB0092 Transferred_Similarity: 100.00% Primary_Match: 100.00%; Adjusted-Interval-0:0/0; QB0092" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc 601 cgcggccgct aatacgactc actataggga gagccgccac catgaaacgg acagccgacg 661 gaagcgagtt cgagtcacca aagaagaagc ggaaagtcag tTCTGAGGTG GAGTTTTCCC 721 ACGAGTACTG GATGAGACAT GCCCTGACCC TGGCCAAGAG GGCACGGGAT GAGGGAGAGG 781 CGCCTGTGGG AGCCGTGCTG GTGCTGAACA ATAGAGTGAT CGGCGAGGGC TGGAACAGAC 841 GTATCGGCCT GCACGACCCA ACAGCCCATG CCGAAATTAT GGCCCTGAGA CAGGGCGGCC 901 TGGTCATGCA GAACTCCAGA CTGATTGACG CCACCCTGTA CGTGACATTC GAGCCTTGCG 961 TGATGTGCGC CGGCGCCATG ATCAACTCTA GGATCGGCCG CGTGGTGTTT GGCGTGAGGA 1021 ACTCAAAAAG AGGCGCCGCA GGCTCCCTGA TGAACGTGCT GAACTACCCC GGCATGAATC 1081 ACCGCGTCGA AATTACCGAG GGAATCCTGG CAGATGAATG TGCCGCCCTG CTGTGCGATT 1141 TCTATCGGAT GCCTAGACAG GTGTTCAATG CTCAGAAGAA GGCCCAGAGC TCCATCAACt 1201 ctggcggatc tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa 1261 caccagagag cagtggcggc agcagcggcg gcagcgggaa gcgaaattac attctggggc 1321 tggccattgg cattacatca gtgggctatg gcatcattga ctacgagaca agggacgtga 1381 tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag ggccggagat 1441 ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag agagtgaaga 1501 agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc atcaatcctt 1561 atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt agcgccgccc 1621 tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag gaggacaccg 1681 gcaacgagct gtccacaaag gagcagatca gccgcaattc caaggccctg gaggagaagt 1741 atgtggccga gctgcagctg gagcggctga agaaggatgg cgaggtgagg ggctccatca 1801 atcgcttcaa gacctctgac tacgtgaagg aggccaagca gctgctgaag gtgcagaagg 1861 cctaccacca gctggatcag tcctttatcg atacatatat cgacctgctg gagacaaggc 1921 gcacatacta tgagggacca ggagagggct ctcccttcgg ctggaaggac atcaaggagt 1981 ggtacgagat gctgatgggc cactgcacct attttccaga ggagctgaga agcgtgaagt 2041 acgcctataa cgccgatctg tacaacgccc tgaatgacct gaacaacctg gtcatcacca 2101 gggatgagaa cgagaagctg gagtactatg agaagttcca gatcatcgag aacgtgttca 2161 agcagaagaa gaagcctaca ctgaagcaga tcgccaagga gatcctggtg aacgaggagg 2221 acatcaaggg ctaccgcgtg acctccacag gcaagccaga gttcaccaat ctgaaggtgt 2281 atcacgatat caaggacatc acagcccgga aggagatcat cgagaacgcc gagctgctgg 2341 atcagatcgc caagatcctg accatctatc agagctccga ggacatccag gaggagctga 2401 ccaacctgaa tagcgagctg acacaggagg agatcgagca gatcagcaat ctgaagggct 2461 acaccggcac acacaacctg agcctgaagg ccatcaatct gatcctggat gagctgtggc 2521 acacaaacga caatcagatc gccatcttta accggctgaa gctggtgcca aagaaggtgg 2581 acctgtccca gcagaaggag atcccaacca cactggtgga cgatttcatc ctgtctcccg 2641 tggtgaagcg gagcttcatc cagagcatca aagtgatcaa cgccatcatc aagaagtacg 2701 gcctgcccaa tgatatcatc atcgagctgg ccagggagaa gaactccaag gacgcccaga 2761 agatgatcaa tgagatgcag aagaggaacc gccagaccaa tgagcggatc gaggagatca 2821 tcagaaccac aggcaaggag aacgccaagt acctgatcga gaagatcaag ctgcacgata 2881 tgcaggaggg caagtgtctg tattctctgg aggccatccc tctggaggac ctgctgaaca 2941 atccattcaa ctacgaggtg gatcacatca tcccccggag cgtgagcttc gacaattctt 3001 ttaacaataa ggtgctggtg aagcaggagg agaacagcaa gaagggcaat aggacccctt 3061 tccagtacct gtctagctcc gattctaaga tcagctacga gacattcaag aagcacatcc 3121 tgaatctggc caagggcaag ggccgcatca gcaagaccaa gaaggagtac ctgctggagg 3181 agcgggacat caacagattc tccgtgcaga aggacttcat caaccggaat ctggtggaca 3241 ccagatacgc cacacgcggc ctgatgaatc tgctgcggtc ttatttcaga gtgaacaatc 3301 tggatgtgaa gGTAaagagc atcaacggcg gcttcacctc ctttctgcgg agaaagtgga 3361 agtttaagaa ggagcgcaac aagggctata agcaccacgc cgaggatgcc ctgatcatcg 3421 ccaatgccga cttcatcttt aaggagtgga agaagctgga caaggccaag aaagtgatgg 3481 agaaccagat gttcgaggag aagcaggccg agagcatgcc cgagatcgag acagagcagg 3541 agtacaagga gattttcatc acacctcacc agatcaagca catcaaggac ttcaaggact 3601 acaagtattc tcacagggtg gataagaagc ccaaccgcga gctgatcaat gacaccctgt 3661 atagcacacg gaaggacgat aagggcaata ccctgatcgt gaacaatctg aacggcctgt 3721 acgacaagga taatgacaag ctgaagaagc tgatcaacaa gtctcccgag aagctgctga 3781 tgtaccacca cgatcctcag acatatcaga agctgaagct gatcatggag cagtacggcg 3841 acgagaagaa cccactgtat aagtactatg aggagacagg caactacctg acaaagtata 3901 gcaagaagga taatggcccc gtgatcaaga agatcaagta ctatggcaac aagctgaatg 3961 cccacctgga catcaccgac gattacccta actctcgcaa taaggtggtg aagctgagcc 4021 tgaagccata ccggttcgac gtgtacctgg acaacggcgt gtataagttt gtgacagtga 4081 agaatctgga tgtgatcaag aaggagaact actatgaggt gaacagcaag tgctacgagg 4141 aggccaagaa gctgaagaag atcagcaacc aggccgagtt catcgcctct ttttacaaca 4201 atgacctgat caagatcaat ggcgagctgt atagagtgat cggcgtgaac aatgatctgc 4261 tgaacagaat cgaagtgaat atgatcgaca tcacctacag ggagtatctg gagaacatga 4321 atgataagag gccccctcgc atcatcaaga ccatcgcctc taagacacag agcatcaaga 4381 agtacagcac agacatcctg gggaacctgt atgaagtcaa gagcaagaaa catcctcaga 4441 ttatcaagaa aggcagcggc gggagcggcg ggagcggggg gagcactaat ctgagcgaca 4501 tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg 4561 aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct 4621 acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc 4681 cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag 4741 gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca 4801 agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg 4861 gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa 4921 atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg 4981 attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg 5041 gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca 5101 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 5161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 5221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 5281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 5341 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc 5401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 5461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg 5521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcggga agaggcggtt tgcgtattgg 5641 gcgcacttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaaa 6421 tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat 6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 6721 accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 7561 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc 7621 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg 7681 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt 7741 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt 7801 acgggccaga tatacgcgtt gacattgatt attgac //