LOCUS       p2T-CMV-SpCas9_TadDE_B 10289 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-152996-sequence-308861_p2T-CMV-evoAmax-BlastR.gbk
            C-to-T base editor.
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1..20
                     /label="T7"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: T7 promoter, forward primer"
     CDS             33..68
                     /label="Translation 33-68"
     misc_feature    complement(65..92)
                     /label=68
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             69..89
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="product: nuclear localization signal of SV40 (simian virus40) large T antigen translation: PKKKRKV"
     CDS             69..89
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    81..92
                     /label="overlap"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    83..92
                     /label=61
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             90..686
                     /label="Translation 90-686"
     misc_feature    93..590
                     /label="TadDE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    591..597
                     /label=21
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    591..603
                     /label=25
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    591..614
                     /label=70
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             687..4787
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system note: generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain translation: DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             687..4787
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             4818..5066
                     /label="UGI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="product: uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995) translation: TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4818..5066
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5067..5096
                     /label="Translation 5067-5096"
     CDS             5097..5345
                     /label="UGI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="product: uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995) translation: TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5097..5345
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5388..5408
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="product: nuclear localization signal of SV40 (simian virus40) large T antigen translation: PKKKRKV"
     CDS             5388..5408
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5417..5434
                     /label="6xHis"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="product: 6xHis affinity tag translation: HHHHHH"
     CDS             5417..5434
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(5457..5474)
                     /label="BGH-rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: Bovine growth hormone terminator, reverse primer. Also called BGH reverse"
     polyA_signal    5463..5687
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: bovine growth hormone polyadenylation signal"
     primer_bind     complement(5758..5774)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer_bind     complement(5758..5774)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: In lacZ gene. Also called M13-rev"
     primer_bind     complement(5771..5793)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: In lacZ gene"
     protein_bind    5782..5798
                     /label="lac operator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5806..5836)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    5851..5872
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     polyA_signal    5989..6110
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 polyadenylation signal"
     primer_bind     complement(6000..6019)
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: SV40 polyA terminator, reverse primer"
     primer_bind     6054..6073
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 polyA, reverse primer"
     CDS             complement(6268..6666)
                     /label="BSD"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="gene: Aspergillus terreus BSD product: blasticidin S deaminase note: confers resistance to blasticidin translation: MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(6268..6666)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(6685..6732)
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: synthetic bacterial promoter "
     promoter        complement(6780..7109)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      6794..6929
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: SV40 origin of replication"
     primer_bind     complement(6848..6867)
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: SV40 promoter/origin, forward primer"
     primer_bind     7094..7114
                     /label="pBABE 3'"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="note: SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    7233..7432
                     /label="Tol2 5' ITR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(7493..8081)
                     /label="ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     complement(7573..7592)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: pBR322 origin, forward primer"
     CDS             complement(8252..9112)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, andrelated antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(8252..9112)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     8875..8894
                     /label="Amp-R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: Ampicillin resistance gene, reverse primer"
     promoter        complement(9113..9217)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: bla"
     misc_feature    9282..9282
                     /label="A>G"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(9296..9315)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: pRS vectors, use to sequence yeast selectable marker"
     misc_feature    9485..9657
                     /label="Tol2 3' ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     enhancer        9665..10044
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: human cytomegalovirus immediate early enhancer"
     misc_feature    9718..9718
                     /label="C>T"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        10045..10248
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     primer_bind     10199..10219
                     /label="CMV-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: Human CMV immediate early promoter, forward primer"
     source          10243..10242
                     /label="source"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="organism: synthetic DNA construct mol_type: other DNA"
ORIGIN
        1 taatacgact cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt
       61 tcgagtcacc aaagaagaag cggaaagtca gtTCTGAGGT GGAGTTTTCC CACGAGTACT
      121 GGATGAGACA TGCCCTGACC CTGGCCAAGA GGGCACGGGA TGAGGGAGAG GCGCCTGTGG
      181 GAGCCGTGCT GGTGCTGAAC AATAGAGTGA TCGGCGAGGG CTGGAACAGA CGTATCGGCC
      241 TGCACGACCC AACAGCCCAT GCCGAAATTA TGGCCCTGAG ACAGGGCGGC CTGGTCATGC
      301 AGAACTCCAG ACTGATTGAC GCCACCCTGT ACGTGACATT CGAGCCTTGC GTGATGTGCG
      361 CCGGCGCCAT GATCAACTCT AGGATCGGCC GCGTGGTGTT TGGCGTGAGG AACTCAAAAA
      421 GAGGCGCCGC AGGCTCCCTG ATGAACGTGC TGAACTACCC CGGCATGAAT CACCGCGTCG
      481 AAATTACCGA GGGAATCCTG GCAGATGAAT GTGCCGCCCT GCTGTGCGAT TTCTATCGGA
      541 TGCCTAGACA GGTGTTCAAT GCTCAGAAGA AGGCCCAGAG CTCCATCAAC tctggcggat
      601 ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga
      661 gcagtggcgg cagcagcggc ggcagcgaca agaagtacag catcggcctg gccatcggca
      721 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
      781 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
      841 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
      901 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
      961 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
     1021 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
     1081 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
     1141 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
     1201 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
     1261 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
     1321 ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga
     1381 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
     1441 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
     1501 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
     1561 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
     1621 ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga
     1681 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
     1741 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
     1801 acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc
     1861 tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc
     1921 agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc
     1981 tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg
     2041 gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca
     2101 tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg
     2161 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
     2221 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
     2281 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     2341 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     2401 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     2461 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     2521 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     2581 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     2641 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     2701 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     2761 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     2821 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     2881 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
     2941 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     3001 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     3061 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     3121 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     3181 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
     3241 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     3301 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     3361 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     3421 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     3481 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     3541 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     3601 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     3661 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     3721 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     3781 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     3841 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     3901 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     3961 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     4021 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     4081 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     4141 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     4201 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     4261 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     4321 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     4381 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     4441 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     4501 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     4561 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     4621 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     4681 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     4741 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgacagc ggcgggagcg
     4801 gcgggagcgg ggggagcact aatctgagcg acatcattga gaaggagact gggaaacagc
     4861 tggtcattca ggagtccatc ctgatgctgc ctgaggaggt ggaggaagtg atcggcaaca
     4921 agccagagtc tgacatcctg gtgcacaccg cctacgacga gtccacagat gagaatgtga
     4981 tgctgctgac ctctgacgcc cccgagtata agccttgggc cctggtcatc caggattcta
     5041 acggcgagaa taagatcaag atgctgagcg gaggatccgg aggatctgga ggcagcacca
     5101 acctgtctga catcatcgag aaggagacag gcaagcagct ggtcatccag gagagcatcc
     5161 tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa gcctgagagc gatatcctgg
     5221 tccataccgc ctacgacgag agtaccgacg aaaatgtgat gctgctgaca tccgacgccc
     5281 cagagtataa gccctgggct ctggtcatcc aggattccaa cggagagaac aaaatcaaaa
     5341 tgctgtctgg cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga
     5401 ggaaagtcta accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     5461 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5521 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5581 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5641 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5701 aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5821 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     5881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctac agacatgata agatacattg
     6001 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6061 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg
     6121 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     6181 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt
     6241 cagtcctgct cctcggccac gaagtgctta gccctcccac acataaccag agggcagcaa
     6301 ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc
     6361 aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt
     6421 ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc
     6481 cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa
     6541 gatgcggccg tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat
     6601 gctgttgatt gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt
     6661 ggccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga
     6721 ttaattgtca acacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt
     6781 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca
     6841 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     6901 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     6961 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     7021 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     7081 ggggactttc cacaccctaa ctgacacaca ttccacagaa ttcactgact cgctgcgctc
     7141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7201 agaatcaggg gataacgcag gaaagaacat gtaagtgatc tccaaaaaat aagtactttt
     7261 tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta
     7321 agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat
     7381 aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc tgcatgtgag
     7441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8101 ctacggggtc tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgggacccac
     8401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9241 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa
     9301 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     9361 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9421 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9481 cgcgggtacc ggcatatggt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca
     9541 agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta
     9601 gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaacg
     9661 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagtttat
     9721 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9781 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9841 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9901 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     9961 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    10021 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
    10081 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
    10141 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
    10201 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
    10261 cgtcagatcc gctagagatc cgcggccgc
//