LOCUS       pDSP16_(empty_vector_un 4703 bp ds-DNA     circular     25-OCT-2022
DEFINITION  .
KEYWORDS    "creator:Dhaval" "marker:KanR"
FEATURES             Location/Qualifiers
     gene            complement(176..690)
                     /label="Split unc-119 5'End"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(691..1675)
                     /label="cbr-unc-119 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     protein_bind    1676..1709
                     /label="loxN"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    1730..1905
                     /label="MCS"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     gene            1939..2768
                     /label="Split mNeonGreen 5' End"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(2931..3519)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(3690..4505)
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(4506..4608)
                     /label="cat promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGTAAAACG
       61 ACGGCCAGTT TATCTAGTCA GCTTGATTCT AGCTGATCGT GGACCGGAAG GTGAGCCAGT
      121 GAGTTGATTG CAGTCCAGTT ACGCTGGAGT CTGAGGCTCG TCCTGAATGA TATGCgttgc
      181 tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt atcttgccga
      241 ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct cgaaaagcac
      301 ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat agatgttcgc
      361 agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa gggagcaaga
      421 taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga gctttcttcg
      481 caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt ggcatctgga
      541 aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata tcaagagtta
      601 gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg ccgagccggg
      661 tggaatcgat Cgttgttgct cggctttcat atcggtttgg ttggaagcgg ctgaaaacgg
      721 aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt agagggtgcc
      781 aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa aatacgataa
      841 tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt tgagtacatg
      901 taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt tttctattta
      961 taacgctaat aatttgaaaa agaaacctAt tgcgaaccgc ggggtgaatc ccaaaaacga
     1021 atgcgttttg gtggagtgat tgattcgaat cgaagaagaa aaagaagaag acgtggaata
     1081 gagagctcac tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa atgaatgagg
     1141 gtcttcttct tcttcttctt cgaatgattg acagaaatgg gaaaaagagg aagattgaga
     1201 agggaaaaag gaaggagaaa agaagcagaa gaagacgtca gagaggagag gaacgagcgg
     1261 aaaagcagcg ggcgcaagtc atagaagtag cagagctggg gagaagaaga cactatccaa
     1321 gaaaggaatg acgagagagt atgcaaaggg gtatagggtg cagacagaat aggaacagaa
     1381 taacagatga tgagccaaga agagttgaaa agggcgatga atttgtcatg taacttaatt
     1441 tgggtcaatt tgagcatgat gaattgaaat catcccttgt tgggagttaa taaccggttt
     1501 gttatcagaa accctgtaat agaagggcgc cctaactttg agccaattca tcccggtttc
     1561 tgtcaaatat atcaaaaagt ggtcaactga caaattgttt ttgatattat aataaacatt
     1621 ttatccgtta acaattttcg aatacttttt acaaggactt ggataaattg gctcaATAAC
     1681 TTCGTATAGT ATACCTTATA CGAAGTTATg taaaacgacg gccagtCCGT AATACGACTC
     1741 ACTtaAGGCC TTGACTAGAG GGTACCAGAG CTCACCTAGG TATCTAGAAC CGGTGACGTC
     1801 ACCATGGGAA GCTTCGTGGA TCCAGATATC CTGCAGGAAT TCCTCGAGAC GTACGGTGCG
     1861 CGCGATGCAT TCGAAGATCT GCCCACTAGT GAGTCGTATT ACGTAGCTTG GCGTAATCAT
     1921 GGTCATAGCT GTTTCCTGAT GGTTTCCAAG GGAGAGGCTG TTATCAAGGA ATTCATGACT
     1981 TACCCAAAGA AGAAGCGTAA GGTCGGAGAA GAGGACAACA TGGCTTCTCT TCCAGCTACT
     2041 CACGAACTTC ATATTTTCGG ATCTATCAAC GGAGTTGATT TCGATATGGT TGGACAAGGA
     2101 ACTGGAAACC CAAACGACGG ATACGAAGAG CTTAACCTTA AGTCTACCAA GGTAAGTTTA
     2161 TCTAAAAAGT TTTTCATTCA AAATGTGTAA AAATTCATTT AAAATAACCC AAAAATCATT
     2221 AATCCTCGAT ATTTTCAGGG AGATCTTCAA TTCTCTCCAT GGATCCTTGT TCCACACATT
     2281 GGATACGGAT TCCACCAATA CCTTCCATAC CCAGATGGAA TGTCTCCATT CCAAGCTGCT
     2341 ATGGTTGACG GATCTGGATA CCAAGTTCAC CGTACTATGC AATTCGAGGA CGGAGCTTCC
     2401 CTCACCGTCA ACTACCGTTA CACTTACGAA GGATCTCACA TCAAGGTATT TTCCTGCATT
     2461 TTTCAACTGG GAAAATGAAA GAAAATCGAT AATTTCAGGG AGAAGCTCAA GTTAAGGGAA
     2521 CCGGATTCCC AGCCGATGGA CCAGTTATGA CCAACTCTCT TACCGCCGCT GATTGGTGCC
     2581 GCTCTAAGAA GACCTACCCA AACGATAAGG TAAGTTTTCT AGAGACCTTT ACTAACTAAC
     2641 TAGGTCTCAT AGATTTTCAG ACTATCATCT CTACTTTCAA GTGGTCTTAC ACTACCGGAA
     2701 ACGGAAAGCG TTACCGCTCT ACCGCTCGCA CTACTTACAC TTTCGCTAAG CCAATGGCTG
     2761 CCAACTACGT ATGATCGGTC CACGATCAGC TAGATTATCT AGTCAGCTGT ATGATCGGTC
     2821 CACGATCAGC TAGATTATCT AGTCAGCTTG ATGTCATAGC TGTTTCCTGA CATGTGAGCA
     2881 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     2941 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     3001 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     3061 CCGACCCTGT CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     3121 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     3181 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     3241 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     3301 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     3361 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     3421 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     3481 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     3541 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     3601 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     3661 AGTATATATG AGTAAACTTG GTCTGACAGT TAGAAAAACT CATCGAGCAT CAAGTGAAAC
     3721 TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT
     3781 GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG
     3841 ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA
     3901 TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA GAGTTTATGC
     3961 ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA
     4021 CCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCCG
     4081 TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA
     4141 TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCT
     4201 GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC
     4261 GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGCCTGACCA TCTCATCTGT AACATCATTG
     4321 GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT
     4381 CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA
     4441 TCAGCATCCA TGTTGGAATT TAATCGCGGC TTCAAGCAAG ACGTTTCCCG TTGAATATGG
     4501 CTCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT
     4561 GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCACA CATTTCCCCG
     4621 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
     4681 GCGTATCACG AGGCCCTTTC GTC
//