LOCUS       Copy_of_pDGB1-a1R-35s-D 4957 bp ds-DNA     circular     05-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      672..1260
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1161..1180
                     /label="pDGB1seq5"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer_bind     1161..1180
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer_bind     1276..1295
                     /label="pDGBseq5b"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    1351..1375
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        1396..1606
                     /label="35S-oTMV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1607..1833
                     /label="35S-oTMV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1835..3250
                     /label="CPPS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1835..3250
                     /label="Translation 1835-3250"
     misc_feature    3255..3551
                     /label="Tnos terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3552..3746
                     /label="Tnos terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     source          3747..3770
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3768..3790
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     source          3771..1391
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(3792..3811)
                     /label="pDGB1seq3"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer_bind     complement(3792..3811)
                     /label="pDGB1seq3"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer_bind     complement(3836..3856)
                     /label="pDGBseq3b"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer_bind     complement(3836..3856)
                     /label="pDGBseq3b"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     rep_origin      3917..4352
                     /label="pSa ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4632..4651)
                     /label="pENTR-R"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     CDS             4643..501
                     /label="KanR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer_bind     complement(4716..4735)
                     /label="Kan-R"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
ORIGIN
        1 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
       61 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
      121 gtatttcgac tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
      181 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
      241 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
      301 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
      361 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
      421 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
      481 atgctcgatg agtttttcta atcactagac caatgttaca catatatact ttagattgat
      541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
      661 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      721 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct tcttccgaag
      781 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      841 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      901 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      961 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1021 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1081 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1141 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1201 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1261 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1321 agatctcaaa caaacacata cagcgactta gtttacccgc caatatatcc tgtcaggatc
     1381 cgtctcatga cGGAGTGAGA CTTTTCAACA AAGGGTAATa tcgggaaacc tcctcggatt
     1441 ccattgccca gctatctgtc acttCatcga aaggacagta gaaaaggaag gtggctccta
     1501 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg
     1561 tcccaaagat ggacccccac ccacgagGAA CATCGTGGAA AAAGAAGATG TTCCAACCAC
     1621 GTCCTCAAAG CAAgtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     1681 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     1741 aggcttcttg agatccttca acaattacca acaacaacaa acaacaaaca acattacaat
     1801 tactatttac aaTTACAGTC GACTCTAGAG GATAATGCCA AAGAAAAAGC GGAAAGTCGG
     1861 AGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
     1921 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
     1981 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
     2041 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
     2101 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
     2161 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TCCAGGACGG
     2221 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     2281 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     2341 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     2401 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     2461 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     2521 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGCG GTGGCGGTAG
     2581 CGGCGGTGGC AGCCTGCAGA TGATCGATAG CAAAGGAGAA GAACTTTTCA CTGGAGTTGT
     2641 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCCGTGGAGA
     2701 GGGTGAAGGT GATGCTACAA TTGGAAAACT CACCCTTAAA TTTATTTGCA CTACTGGAAA
     2761 ACTACCTGTT CCATGGCCAA CACTTGTCAC TACTCTGACC TATGGTGTTC AATGCTTTTC
     2821 CCGTTATCCG GATCACATGA AACGGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA
     2881 TGTACAGGAA CGCACTATAT CTTTCAAAGA TGACGGGAAA TACAAGACGC GTGCTGTCGT
     2941 CAAGTTTGAA GGTGATACCC TTGTTAATCG TATCGAGTTA AAAGGTACTG ATTTTAAAGA
     3001 AGATGGAAAC ATTCTCGGAC ACAAACTCGA GTACAACTTT AACTCACACA ATGTATACAT
     3061 CACGGCAGAC AAACAAAAGA ATGGAATCAA AGCTAACTTC ACCGTTCGCC ACAACGTTGA
     3121 AGATGGTTCC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC
     3181 TGTCCTTTTA CCAGACAACC ATTACCTGTC GACACAAACT GTCCTTTCGA AAGATCCCAA
     3241 CGAAAAGTGA GCTTGGAATG GATCTTCGAT CCCGatcgtt caaacatttg gcaataaagt
     3301 ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt tctgttgaat
     3361 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3421 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3481 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attGCCAAGC
     3541 TAATTCTTGA AGATGAAAGG GCCTCGTGAT Acgcctattt ttataggtta atgtcatgat
     3601 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3661 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3721 aatgctTCAA TAATGGGACC GACTCGcgct ctcctgagac ggatccgtta caccacaata
     3781 tatcctgcca agatctaatt ccggggatcg gaaatccaga agcccgagag gttgccgcct
     3841 ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc ggccggccgc
     3901 cgggttgtgg gcaaaggcgc tcgacggtgg gcaaccgctt gcggttgtcc acgggcggag
     3961 ccggtgcgcg tagcgcattg tccacaagcc aagggcgacc aataattgat atatatattc
     4021 ataattgaaa agctaattga acatactact tgctgtaact acttgccgga gcgaggggtg
     4081 tttgcaagct gttgatctga aagggctatt agcgttctca cgtgcctttt tgattagcga
     4141 tttcacgtga ccttattagc gatttcacgt actccgatta gcgatttcac gtaccctgat
     4201 tagcgatttc acgtggatag tttttggagc gggccggaaa gccccgtgaa tcaaggcttt
     4261 gcggggcatt agcggtttca cgtggataac taccctctat ccacaggctt ccggggataa
     4321 aaaagcccgc tcgacggcgg gctgttggat ggggatcgcc tgaatcgccc catcatccag
     4381 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
     4441 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
     4501 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg
     4561 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     4621 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcaaggcc
     4681 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     4741 cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt
     4801 tctgaaacat ggcaaaggta gcgttgccaa tgttgttaca gatgagatgg tcagactaaa
     4861 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     4921 tgcatggtta ctcaccactg cgatcccagg gaaaaca
//