LOCUS       (559)pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N        8367 bp    DNA     circular UNA 27-OCT-2022
DEFINITION  .
ACCESSION   urn.local...m-fh5nv34
VERSION     urn.local...m-fh5nv34
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             916..3930
                     /codon_start=1
                     /transl_table=1
                     /translation="MEQKLISEEDLAIAMVQKESQATLEERESELSSNPAASAGASLE
                     PPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKW
                     ELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFS
                     NPKEVSTLKRKLDQVSQFGCRSFALLFNDIDHNMCLAEGTRIFDPVTGTTHRIEDVVD
                     GRKPIHVVAAAKDGTLLARPVVSWFDQGTRDVIGLRIAGGAIVWATPDHKVLTEYGWR
                     AAGELRKGDRVAGPGGSGNSLALSLTADQMVSALLDAEPPILYSEYDPTSPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQAHLLERAWLEILMIGLVWRSMEHPGK
                     LLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVY
                     TFLSSTLKSLEEKDHIHRALDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHM
                     SNKGMEHLYSMKYKNVVPLYDLLLEMLDAHRLHAGGSGASRVQAFADALDDKFLHDML
                     AEGLRYSVIREVLPTRRARTFDLEVEELHTLVAEGVVVHNCAADKEVFSSFAHAQVSI
                     TNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVS
                     KEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNP
                     NCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDV
                     LYSPQMALKLALTEWLQEFGVPHQYSSRGSGSGSGSGSGSGSGEKPLYTAEPVTLEDL
                     QLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEE
                     MCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIG
                     DQEPWAFRGGLAGEFQRLLPIDGANDLFFQPP*"
                     /label="myc-miniOGA-C181intein-D174N"
     CDS             919..948
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    1477..1479
                     /label="D174N"
     misc_feature    1498..2736
                     /label="intein"
     polyA_signal    3967..4191
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4237..4665
                     /snapgene_direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4679..5008
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      4859..4994
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     CDS             5075..5869
                     /codon_start=1
                     /gene="<i>aph(3')-II</i> (or <i>nptII</i>)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6043..6164
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6213..6229)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6237..6253
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        join(complement(6286..6291),complement(6268..6285),
                     complement(6261..6267))
                     /note="promoter for the <i>E. coli lac</i> operon"
                     /label="lac promoter"
     protein_bind    6306..6327
                     /bound_moiety="<i>E. coli</i> catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6615..7200)
                     /snapgene_direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             join(complement(8163..8231),complement(7371..8162))
                     /codon_start=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR"
     promoter        complement(8232..8336)
                     /gene="<i>bla</i>"
                     /label="AmpR promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggcgagc
      901 gtttaagctt gagcaatgga gcagaagctg atcagcgagg aggatctggc gatcgcaatg
      961 gtgcagaagg agagtcaagc gacgttggag gagcgggaga gcgagctcag ctccaaccct
     1021 gccgcctctg cgggggcatc gctggagccg ccggcagctc cggcacccgg agaagacaac
     1081 cccgccgggg ctgggggagc ggcggtggcc ggggctgcag gaggggctcg gcggttcctc
     1141 tgcggtgtgg tggaaggatt ttatggaaga ccttgggtta tggaacagag aaaagaactc
     1201 tttagaaggc tccagaaatg ggaattaaat acatacttgt atgccccaaa agatgactac
     1261 aaacatagga tgttttggcg agagatgtat tcagtggagg aagctgagca acttatgact
     1321 ctcatctctg ctgcacgaga atatgagata gagttcatct atgcgatctc acctggattg
     1381 gatatcactt tttctaaccc caaggaagta tccacattga aacgtaaatt ggaccaggtt
     1441 tctcagtttg ggtgcagatc atttgctttg ctttttaatg atatagacca taatatgtgc
     1501 cttgccgagg gtacccgaat cttcgatccg gtcactggta caacgcatcg catcgaggat
     1561 gttgtcgatg ggcgcaagcc tattcatgtc gtggctgctg ccaaggacgg aacgctgctc
     1621 gcgcggcccg tggtgtcctg gttcgaccag ggaacgcggg atgtgatcgg gttgcggatc
     1681 gccggtggcg ccatcgtgtg ggcgacaccc gatcacaagg tgctgacaga gtacggctgg
     1741 cgtgccgccg gggaactccg caagggagac agggtggccg gaccgggtgg ttctggtaac
     1801 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
     1861 cccatactct attccgagta tgatcctacc agtcccttca gtgaagcttc aatgatgggc
     1921 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     1981 ccaggctttg tggatttgac cctccatgat caggcccacc ttctagaacg tgcctggcta
     2041 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagggaa gctactgttt
     2101 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     2161 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     2221 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     2281 accctgaagt ctctggaaga gaaggaccat atccaccgag ccctggacaa gatcacggac
     2341 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     2401 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg aatggagcat
     2461 ctgtacagca tgaagtacaa gaacgtggtg cccctctatg acctgctgct ggagatgctg
     2521 gacgcccacc gcctacatgc gggtggttct ggtgctagcc gcgtgcaggc gttcgcggat
     2581 gccctggatg acaaattcct gcacgacatg ctggcggaag gactccgcta ttccgtgatc
     2641 cgagaagtgc tgccaacgcg gcgggcacga acgttcgacc tcgaggtcga ggaactgcac
     2701 accctcgtcg ccgaaggggt tgtcgtgcac aactgcgcag cagacaaaga ggtattcagt
     2761 tcttttgctc atgcccaagt ctccatcaca aatgaaatct atcagtacct aggagagcca
     2821 gaaactttcc tcttctgtcc cacagaatac tgtggcactt tctgttatcc aaatgtgtct
     2881 cagtctccat atttaaggac tgtgggtgaa aagcttctac ctggaattga agtgctttgg
     2941 acaggtccca aagttgtttc taaagaaatt ccagtagagt ccatcgaaga ggtttctaag
     3001 attattaaga gagctccagt aatctgggat aacattcatg ctaatgatta tgatcagaag
     3061 agactgtttc tgggcccgta caaaggaaga tccacagaac tcatcccacg gttaaaagga
     3121 gtcctcacta atccaaattg tgaatttgaa gccaactacg ttgctatcca cacccttgcc
     3181 acctggtaca aatcaaacat gaatggagtg agaaaagatg tagtgatgac tgacagtgaa
     3241 gatagtactg tgtccatcca gataaaatta gaaaatgaag gcagtgatga agatattgaa
     3301 actgatgtac tctatagtcc acagatggct ctaaagctag cattaacaga atggttgcaa
     3361 gagtttggtg tgcctcatca atacagcagt aggggaagcg gctccggaag tggatctgga
     3421 agtggatccg gaagcggaga aaagcctttg tacactgcgg aaccagtgac cctggaggat
     3481 ttgcagttac ttgctgatct attctacctt ccttacgagc atggacccaa aggagcacag
     3541 atgttacggg aatttcaatg gcttcgagca aatagtagtg ttgtcagtgt caattgcaaa
     3601 ggaaaagact ctgaaaaaat tgaagaatgg cggtcacgag cagccaagtt tgaagagatg
     3661 tgtggactag tgatgggaat gttcactcgg ctctccaatt gtgccaacag gacaattctt
     3721 tatgacatgt actcctatgt ttgggatatc aagagtataa tgtctatggt gaagtctttt
     3781 gtacagtggt tagggtgtcg tagtcattct tcagcacaat tcttaattgg agaccaagaa
     3841 ccctgggcct ttagaggtgg tctagcagga gagttccagc gtttgctgcc aattgatggg
     3901 gcaaatgatc tcttttttca gccaccttaa gtctagaggg cccgtttaaa cccgctgatc
     3961 agccgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4021 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4081 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4141 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4201 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4441 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4681 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4741 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4801 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4921 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4981 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5041 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5101 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5161 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5221 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5281 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5341 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5401 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5461 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5521 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5581 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5641 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5701 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5761 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5821 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5881 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5941 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6001 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7141 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7321 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7681 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7981 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8341 tttccccgaa aagtgccacc tgacgtc
//
