LOCUS       OA-1163A-Ubiq_Rf       11164 bp DNA     circular SYN 31-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2022 from SnapGene 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(854..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     old_sequence    2319..3026
                     /label=flanking sequence
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
     misc_binding    4073..4357
                     /label=attB site
                     /note="attB site"
     misc_feature    4366..4786
                     /label=AAEL006511 Ubiquitin-L-40 Promoter
                     /note="AAEL006511 Ubiquitin-L-40 Promoter region from 
                     Anderson et. Al 2010"
     CDS             4794..7826
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d-NES-3xHA
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNSGSLQLPPLERLTLGSSYPYDVP
                     DYAYPYDVPDYAYPYDVPDYA"
     CDS             4794..7694
                     /codon_start=1
                     /label=RfxCas13d-codon optimized for Aedes
                     /note="RfxCas13d"
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7707..7733
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7743..7823
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    7743..7769
                     /label=HA
     misc_feature    7770..7796
                     /label=HA
     misc_feature    7797..7823
                     /label=HA
     misc_feature    7832..8508
                     /label=p10-3'-UTR-Rubin
                     /note="p10-3'-UTR-Rubin"
     misc_feature    8517..9057
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
     misc_feature    8517..8978
                     /label=phi
                     /note="phi"
     misc_feature    9058..9738
                     /label=Dsred
                     /note="Dsred"
     CDS             9058..9738
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     CDS             9058..9735
                     /label=DsRed (RFP)
     misc_feature    9385..9393
                     /label=pt mutation
                     /note="pt mutation"
     misc_feature    9739..9979
                     /label=Sv40
                     /note="Sv40"
     polyA_signal    9852..9973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_binding    9991..10691
                     /label=piggyBacL
     old_sequence    10692..10820
                     /label=flanking seq
     misc_feature    complement(10821..11164)
                     /label=misc_feature 1
     primer_bind     complement(10857..10873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10881..10897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10905..10935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10950..10971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggtcgacga
     4081 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     4141 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
     4201 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
     4261 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
     4321 tcagcgggtt ctcgacggtc acggcgggca tgtcgacgcg gccgcagcgt gaaccttgca
     4381 tgcgtgcaca gaatttgatt gcatttcatg accgggtatc cattcggcgc cggccactga
     4441 actgaaattc agttttgaca gctccactga cttgaagctc tttttcaccg aaacacatct
     4501 cggaagagac acgttgggaa agagtaagtt taatttttgt cgtttttcgt ggatttttag
     4561 tgataattcg tggtttttcg ttgttagcaa tctaaatcat tgatagatca agttagaaat
     4621 tgtgaaatat tgctaaaata cctacattat tcgtagttta ttcgatttgt tgtagttgat
     4681 gcattctaac ctcaatgtgg tttcgtctcc gtccaattcc gatgttattg tccagttcgt
     4741 taatttttgg tgttttctcc ccgacttttc cacagactga tccaagttaa ttaatgatcg
     4801 aaaagaagaa gtcgttcgcc aagggaatgg gagtgaagtc gaccctggtg tcgggatcga
     4861 aggtgtacat gaccaccttc gccgaaggat cggatgcccg tctggaaaag atcgtggaag
     4921 gagattcgat ccgttcggtg aacgaaggag aagccttctc ggccgaaatg gccgataaga
     4981 acgccggata caagatcgga aacgccaagt tctcgcaccc gaagggatac gccgtggtgg
     5041 ccaacaaccc gctgtacacc ggaccggtgc agcaggatat gctgggactg aaggaaaccc
     5101 tggaaaagcg ttacttcgga gaatcggccg atggaaacga taacatctgc atccaggtga
     5161 tccacaacat cctggatatc gaaaagatcc tggccgaata catcaccaac gccgcctacg
     5221 ccgtgaacaa catctcggga ctggataagg atatcatcgg attcggaaag ttctcgaccg
     5281 tgtacaccta cgatgaattc aaggatccgg aacaccaccg tgccgccttc aacaacaacg
     5341 ataagctgat caacgccatc aaggcccagt acgatgaatt cgataacttc ctggataacc
     5401 cgcgtctggg atacttcgga caggccttct tctcgaagga aggacgtaac tacatcatca
     5461 actacggaaa cgaatgctac gatatcctgg ccctgctgtc gggactgcgt cactgggtgg
     5521 tgcacaacaa cgaagaagaa tcgcgtatct cgcgtacctg gctgtacaac ctggataaga
     5581 acctggataa cgaatacatc tcgaccctga actacctgta cgatcgtatc accaacgaac
     5641 tgaccaactc gttctcgaag aactcggccg ccaacgtgaa ctacatcgcc gaaaccctgg
     5701 gaatcaaccc ggccgaattc gccgaacagt acttccgttt ctcgatcatg aaggaacaga
     5761 agaacctggg attcaacatc accaagctgc gtgaagtgat gctggatcgt aaggatatgt
     5821 cggaaatccg taagaaccac aaggtgttcg attcgatccg taccaaggtg tacaccatga
     5881 tggatttcgt gatctaccgt tactacatcg aagaagatgc caaggtggcc gccgccaaca
     5941 agtcgctgcc ggataacgaa aagtcgctgt cggaaaagga tatcttcgtg atcaacctgc
     6001 gtggatcgtt caacgatgat cagaaggatg ccctgtacta cgatgaagcc aaccgtatct
     6061 ggcgtaagct ggaaaacatc atgcacaaca tcaaggaatt ccgtggaaac aagacccgtg
     6121 aatacaagaa gaaggatgcc ccgcgtctgc cgcgtatcct gccggccgga cgtgatgtgt
     6181 cggccttctc gaagctgatg tacgccctga ccatgttcct ggatggaaag gaaatcaacg
     6241 atctgctgac caccctgatc aacaagttcg ataacatcca gtcgttcctg aaggtgatgc
     6301 cgctgatcgg agtgaacgcc aagttcgtgg aagaatacgc cttcttcaag gattcggcca
     6361 agatcgccga tgaactgcgt ctgatcaagt cgttcgcccg tatgggagaa ccgatcgccg
     6421 atgcccgtcg tgccatgtac atcgatgcca tccgtatcct gggaaccaac ctgtcgtacg
     6481 atgaactgaa ggccctggcc gataccttct cgctggatga aaacggaaac aagctgaaga
     6541 agggaaagca cggaatgcgt aacttcatca tcaacaacgt gatctcgaac aagcgtttcc
     6601 actacctgat ccgttacgga gatccggccc acctgcacga aatcgccaag aacgaagccg
     6661 tggtgaagtt cgtgctggga cgtatcgccg atatccagaa gaagcaggga cagaacggaa
     6721 agaaccagat cgatcgttac tacgaaacct gcatcggaaa ggataaggga aagtcggtgt
     6781 cggaaaaggt ggatgccctg accaagatca tcaccggaat gaactacgat cagttcgata
     6841 agaagcgttc ggtgatcgaa gataccggac gtgaaaacgc cgaacgtgaa aagttcaaga
     6901 agatcatctc gctgtacctg accgtgatct accacatcct gaagaacatc gtgaacatca
     6961 acgcccgtta cgtgatcgga ttccactgcg tggaacgtga tgcccagctg tacaaggaaa
     7021 agggatacga tatcaacctg aagaagctgg aagaaaaggg attctcgtcg gtgaccaagc
     7081 tgtgcgccgg aatcgatgaa accgccccgg ataagcgtaa ggatgtggaa aaggaaatgg
     7141 ccgaacgtgc caaggaatcg atcgattcgc tggaatcggc caacccgaag ctgtacgcca
     7201 actacatcaa gtactcggat gaaaagaagg ccgaagaatt cacccgtcag atcaaccgtg
     7261 aaaaggccaa gaccgccctg aacgcctacc tgcgtaacac caagtggaac gtgatcatcc
     7321 gtgaagatct gctgcgtatc gataacaaga cctgcaccct gttccgtaac aaggccgtgc
     7381 acctggaagt ggcccgttac gtgcacgcct acatcaacga tatcgccgaa gtgaactcgt
     7441 acttccagct gtaccactac atcatgcagc gtatcatcat gaacgaacgt tacgaaaagt
     7501 cgtcgggaaa ggtgtcggaa tacttcgatg ccgtgaacga tgaaaagaag tacaacgatc
     7561 gtctgctgaa gctgctgtgc gtgccgttcg gatactgcat cccgcgtttc aagaacctgt
     7621 cgatcgaagc cctgttcgat cgtaacgaag ccgccaagtt cgataaggaa aagaagaagg
     7681 tgtcgggaaa ctcgggatcc cttcaactgc ctccacttga aagactgaca ctgggtagtt
     7741 cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc
     7801 catatgatgt ccccgactat gcctaagcta gctagaatga atcgttttta aaataacaaa
     7861 tcaattgttt tataatattc gtacgattct ttgattatgt aataaaatgt gatcattagg
     7921 aagattacga aaaatataaa aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc
     7981 acaattgtgt ttgttgcaaa taaacccatg attatttgat taaaattgtt gttttctttg
     8041 ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca taaactccat gcgagtgtat
     8101 agcgagctag tggctaacgc ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca
     8161 tcaccctcct ccaagtttaa catttggccg tcggaattaa cttctaaaga tgccacataa
     8221 tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa
     8281 aagaactcgg gcataaactc tatgatttct ctggacgtgg tgttgtcgaa actctcaaag
     8341 tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact cgcgcggaaa catgttgttg
     8401 taaccgaacg ggtcccatag cgccaaaacc aaatctgcca gcgtcaatag aatgagcacg
     8461 atgccgacaa tggagctggc ttggatagcg attcgagtta acggccgggg cgcgcccata
     8521 ctcggtggcc tccccaccac caactttttt gcactgcaaa aaaacacgct tttgcacgcg
     8581 ggcccataca tagtacaaac tctacgtttc gtagactatt ttacataaat agtctacacc
     8641 gttgtatacg ctccaaatac actaccacac attgaacctt tttgcagtgc aaaaaagtac
     8701 gtgtcggcag tcacgtaggc cggccttatc gggtcgcgtc ctgtcacgta cgaatcacat
     8761 tatcggaccg gacgagtgtt gtcttatcgt gacaggacgc cagcttcctg tgttgctaac
     8821 cgcagccgga cgcaactcct tatcggaaca ggacgcgcct ccatatcagc cgcgcgttat
     8881 ctcatgcgcg tgaccggaca cgaggcgccc gtcccgctta tcgcgcctat aaatacagcc
     8941 cgcaacgatc tggtaaacac agttgaacag catctgttac agcgacacaa catgagccgg
     9001 tccaacaacg ccaacgcgcc cacgccatcc aaccgccgcc gcaacctgtc tctggtgatg
     9061 gtgcgctcct ccaagaacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
     9121 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     9181 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     9241 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
     9301 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     9361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
     9421 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagacc
     9481 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     9541 atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
     9601 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac
     9661 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
     9721 caccacctgt tcctgtagga ctctagatca taatcagcca taccacattt gtagaggttt
     9781 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     9841 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9961 tcaatgtatc ttaaagcttc taggccggcc gatctcggat ctgacaatgt tcagtgcaga
    10021 gactcggcta cgcctcgtgg actttgaagt tgaccaacaa tgtttattct tacctctaat
    10081 agtcctctgt ggcaaggtca agattctgtt agaagccaat gaagaacctg gttgttcaat
    10141 aacattttgt tcgtctaata tttcactacc gcttgacgtt ggctgcactt catgtacctc
    10201 atctataaac gcttcttctg tatcgctctg gacgtcatct tcacttacgt gatctgatat
    10261 ttcactgtca gaatcctcac caacaagctc gtcatcgctt tgcagaagag cagagaggat
    10321 atgctcatcg tctaaagaac tacccatttt attatatatt agtcacgata tctataacaa
    10381 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
    10441 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
    10501 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
    10561 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
    10621 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaaa
    10681 aaagatttgc gctttactcg acctaaactt taaacacgtc atagaatctt cgtttgacaa
    10741 aaaccacatt gtggccaagc tgtgtgacgc gacgcgcgct aaagaatggc aaaccaagtc
    10801 gcgcgagcgt cgactctaga ggatccccgg gtaccgagct cgaattcgta atcatggtca
    10861 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    10921 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    10981 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    11041 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    11101 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11161 cggt
//
