LOCUS       C16Ex4_pYTK084_construc 3867 bp ds-DNA     circular     14-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(26..41)
                     /label="primer_07_insert"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    26..72
                     /label="primer_03"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    42..52
                     /label="primer_01"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    42..52
                     /label="new_sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    53..72
                     /label="primer348_attachment"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    53..1809
                     /label="Backbone"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(82..897)
                     /label="KanR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="gene: aph(3')-Ia product: aminoglycoside phosphotransferase note: confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(82..897)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     805..824
                     /label="Kan-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: Kanamycin resistance gene, reverse primer"
     primer_bind     889..908
                     /label="pENTR-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: pENTR vectors, reverse primer"
     rep_origin      1122..1710
                     /label="ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     1611..1630
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: pBR322 origin, forward primer"
     misc_feature    1790..1809
                     /label="primer349_attachment"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1790..1823)
                     /label="primer_02"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1790..1837)
                     /label="primer_04"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1810..1823
                     /label="new_sequence"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1824..41
                     /label="Insert"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1824..1839
                     /label="primer_08_insert"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1824..1922
                     /label="L arm"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="locus_tag: L arm label: L arm ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1827..1842
                     /label="primer_08_insert"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1920..1922
                     /label="5' guide"
                     /ApEinfo_revcolor="#ff40ff"
                     /ApEinfo_fwdcolor="#ff40ff"
                     /note="locus_tag: 5' guide label: 5' guide ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1923..1956
                     /label="Lox2272"
                     /ApEinfo_revcolor="#aa7941"
                     /ApEinfo_fwdcolor="#aa7941"
                     /note="locus_tag: Lox2272 label: Lox2272 ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1923..2004
                     /label="5' Lox2272-spacer-LoxP511"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: 5' Lox2272-spacer-LoxP511 label: 5' Lox2272-spacer-LoxP511 ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1971..2004
                     /label="Lox5171"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="locus_tag: Lox5171 label: Lox5171 ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2005..2010
                     /label="HindIII"
                     /ApEinfo_revcolor="#aa7941"
                     /ApEinfo_fwdcolor="#aa7941"
                     /note="locus_tag: HindIII label: HindIII ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2011..2027
                     /label="5' guide"
                     /ApEinfo_revcolor="#ff40ff"
                     /ApEinfo_fwdcolor="#ff40ff"
                     /note="locus_tag: 5' guide(1) label: 5' guide(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2106..2384
                     /label="Exon 4"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="locus_tag: Exon 4 label: Exon 4 ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2531..2533
                     /label="3' guide"
                     /ApEinfo_revcolor="#ff40ff"
                     /ApEinfo_fwdcolor="#ff40ff"
                     /note="locus_tag: 3' guide label: 3' guide ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2534..2565
                     /label="Rox"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="locus_tag: Rox label: Rox ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2566..3663
                     /label="XTR f1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: XTR f1 label: XTR f1 ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3584..3585
                     /label="f1 GG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="locus_tag: f1 GG label: f1 GG ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3664..3695
                     /label="Rox"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="locus_tag: Rox(1) label: Rox(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3696..3729
                     /label="5' Lox2272-spacer-LoxP511"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: 5' Lox2272-spacer-LoxP511(2) label: 5' Lox2272-spacer-LoxP511(2) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3696..3729
                     /label="Lox2272"
                     /ApEinfo_revcolor="#aa7941"
                     /ApEinfo_fwdcolor="#aa7941"
                     /note="locus_tag: Lox2272(1) label: Lox2272(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3730..3775
                     /label="XTR f1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: XTR f1(1) label: XTR f1(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3776..3809
                     /label="Lox5171"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="locus_tag: Lox5171(1) label: Lox5171(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3776..3809
                     /label="5' Lox2272-spacer-LoxP511"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: 5' Lox2272-spacer-LoxP511(1) label: 5' Lox2272-spacer-LoxP511(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3810..41
                     /label="R arm"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="locus_tag: R arm label: R arm ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3810..3826
                     /label="3' guide"
                     /ApEinfo_revcolor="#ff40ff"
                     /ApEinfo_fwdcolor="#ff40ff"
                     /note="locus_tag: 3' guide(1) label: 3' guide(1) ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
ORIGIN
        1 catatcctct gccttgaggt cagacggagt gacaaatgag cGCTGTGAGA CCgcgtgtta
       61 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
      121 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
      181 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
      241 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
      301 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
      361 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
      421 accgttattc attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg
      481 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
      541 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
      601 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
      661 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
      721 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
      781 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
      841 catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac
      901 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
      961 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     1021 aacttttgct gagttgaagg atcagtcatg accaaaatcc cttaacgtga gttttcgttc
     1081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1261 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1801 gataaccgtG GTCTCTGCTG AGGaggggaa gtgagtctca agattgcagt ggtagaagtc
     1861 aagatgtgat tgggaaagac tcaagttctt tctgccctct actcagccct gccaacccca
     1921 ggATAACTTC GTATAGGATA CCTTATACGA AGTTATCTCA GCTAGAAGCC ATAACTTCGT
     1981 ATAGTACACA TTATACGAAG TTATAAGCTT gacccaatcc ggtagattga agacctgagg
     2041 tgcatgcctc tgtcccctcc aaccccactc ctgcccctcc tgtgtctctc cctcctgccc
     2101 tacagTCGGC CCTGAACGAG TAGCTATCCT TCAAGACTCT ACCACCCGCA CGGGCTGCAC
     2161 TATTAAAGTG GACTTCAACA TGTCCCTCAC CCTGTGGTGT GTGGCGCGGT CTTGCCCTGA
     2221 GCCAGAGTAC GTGTGGGCCT TCAATGGAAA GGCCTTGAAG AATGGCCAGG ATCACCTGAA
     2281 CATCAGCAGT ATGACTGCAG CCCATGAGGG CACGTACACG TGCATCGCTA AGAACTCCAA
     2341 GACGCTGCTG TCTGGCTCTG CCTCAGTGGT AGTCAAGCTG TCCGgtgagt cagccccagc
     2401 ctgatagcca cctgctctct agagaggccc agctggcctt tgctgactgg gctgccattt
     2461 tccagaaaat aattggattc ccagcacact cattggccag ttgtgacacg gtggcgtatc
     2521 agattgccct gtcTAACTTT AAATAATGCC AATTATTTAA AGTTAGTTTA TCGAATTCCT
     2581 GCAGCCCGGT AGATCTTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC
     2641 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
     2701 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
     2761 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
     2821 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CCTTAtTTGT ACAGCTCGTC
     2881 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
     2941 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG
     3001 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC
     3061 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC
     3121 CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
     3181 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
     3241 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
     3301 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
     3361 GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG
     3421 CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC
     3481 GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT
     3541 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATCCCTGGAA AAAAGAAATG
     3601 CGAAGATTGC GCCTGCGCAT CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGCC
     3661 CGGTAACTTT AAATAATGCC AATTATTTAA AGTTAATAAC TTCGTATAGG ATACCTTATA
     3721 CGAAGTTATC CATTCAGGCT GTGCTAGCAT CAATGGCATG GCACAAAGCT TAGCCATAAC
     3781 TTCGTATAGT ACACATTATA CGAAGTTATt tgtctaggtg cggaaagagg caggcaaagc
     3841 ctattgcata ggaaagaaat ggtgact
//