LOCUS       Circular        4453 bp DNA     circular SYN 18-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    379..395
                     /locus_tag="M13_F primer"
                     /label=M13_F primer
     misc_feature    405..423
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
     misc_feature    500..844
                     /locus_tag="CaMV 35S promoter"
                     /label=CaMV 35S promoter
     misc_feature    864..919
                     /locus_tag="TMV I(C)"
                     /label=TMV Omega
                     /label=TMV I(C)
     misc_feature    989
                     /locus_tag="Gene insert spot between c and t"
                     /label=Gene insert spot between c and t
     misc_feature    990..1139
                     /locus_tag="AtTIM21(1-50aa)"
                     /label=AtTIM21(1-50aa)
     misc_feature    1140..1847
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    1848..1853
                     /locus_tag="stop codon"
                     /label=stop codon
     misc_feature    1854
                     /locus_tag="Gene insert spot between c and t(1)"
                     /label=Gene insert spot between c and t
                     /label=Gene insert spot between c and t(1)
     misc_feature    1927..2177
                     /locus_tag="NOS terminator"
                     /label=NOS terminator
     misc_feature    2242..2260
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
     misc_feature    2278..2294
                     /locus_tag="M13 R_primer"
                     /label=M13 R_primer
     misc_feature    2302..2318
                     /locus_tag="lac operator"
                     /label=lac operator
     misc_feature    2326..2356
                     /locus_tag="lac promoter"
                     /label=lac promoter
     misc_feature    2371..2392
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     misc_feature    2680..3268
                     /locus_tag="ori"
                     /label=ori
     misc_feature    3426..4241
                     /locus_tag="KanR"
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaatattac tacaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgagct agtgtaatac gactcactat
      421 agggcgcggc cgcagaattc gagctcggta cccgggatct cgaggccaga tctcccgggc
      481 tcagaagacc gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      541 gattccattg cccagctatc tgtcacttat cgaaaggaca gtagaaaagg aaggtggctc
      601 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag
      661 tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac
      721 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      781 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
      841 gacaggcttc ttgagatcct tcaacaatta ccaacaacaa caaacaacaa acaacattac
      901 aattactatt tacaattaca gaattcgagc tcggtaccaa gcttggaggc tcaggaggct
      961 ccacaagttt gtacaaaaaa gcaggcttca tgatgatgat gaatctttta aggagatcag
     1021 cgatagccat cggccgccaa tcaaaatcga aactggcgtc gttttcctcc gctacacaac
     1081 cttgttccgg aatcccaaaa tcgtccaaga gggtttttag caactcattt ctttcaaaga
     1141 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     1201 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1261 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     1321 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     1381 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     1441 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     1501 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1561 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1621 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1681 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     1741 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1801 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag tgatgagacc
     1861 cagctttctt gtacaaagtg gttggaggct caggaggctc cccatgggct agcactagta
     1921 tctagaatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     1981 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     2041 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     2101 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     2161 catctatgtt actagatgcg gccgcaattg tggatccgct ctagagtcga cctgcaggca
     2221 tgcaagcttg cggccgcgta ttctatagtg tcacctaaat agcatggcgt aatcatggtc
     2281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3121 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt
     3421 tttaattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     3481 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     3541 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     3601 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     3661 acgactgaat ccggtgagaa tggcaatagc ttatgcattt ctttccagac ttgttcaaca
     3721 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     3781 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     3841 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     3901 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     3961 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     4021 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     4081 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     4141 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     4201 cgcggccttg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     4261 tttatgtaag cagacataag catttatcag ggttattgtc tcatgagcgg atacatattt
     4321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4381 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4441 aggccctttc gtc
//
