LOCUS       Circular        4327 bp DNA     circular SYN 18-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    379..395
                     /locus_tag="M13_F primer"
                     /label=M13_F primer
     misc_feature    405..423
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
     misc_feature    500..844
                     /locus_tag="CaMV 35S promoter"
                     /label=CaMV 35S promoter
     misc_feature    864..919
                     /locus_tag="TMV I(C)"
                     /label=TMV Omega
                     /label=TMV I(C)
     misc_feature    989
                     /locus_tag="Gene insert spot between c and t"
                     /label=Gene insert spot between c and t
     misc_feature    990..1697
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    1704..1724
                     /locus_tag="NLS_SV40"
                     /label=NLS_SV40
     misc_feature    1725..1727
                     /locus_tag="stop codon"
                     /label=stop codon
     misc_feature    1728
                     /locus_tag="Gene insert spot between c and t(1)"
                     /label=Gene insert spot between c and t
                     /label=Gene insert spot between c and t(1)
     misc_feature    1801..2051
                     /locus_tag="NOS terminator"
                     /label=NOS terminator
     misc_feature    2116..2134
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
     misc_feature    2152..2168
                     /locus_tag="M13 R_primer"
                     /label=M13 R_primer
     misc_feature    2176..2192
                     /locus_tag="lac operator"
                     /label=lac operator
     misc_feature    2200..2230
                     /locus_tag="lac promoter"
                     /label=lac promoter
     misc_feature    2245..2266
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     misc_feature    2554..3142
                     /locus_tag="ori"
                     /label=ori
     misc_feature    3300..4115
                     /locus_tag="KanR"
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaatattac tacaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgagct agtgtaatac gactcactat
      421 agggcgcggc cgcagaattc gagctcggta cccgggatct cgaggccaga tctcccgggc
      481 tcagaagacc gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      541 gattccattg cccagctatc tgtcacttat cgaaaggaca gtagaaaagg aaggtggctc
      601 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag
      661 tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac
      721 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      781 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
      841 gacaggcttc ttgagatcct tcaacaatta ccaacaacaa caaacaacaa acaacattac
      901 aattactatt tacaattaca gaattcgagc tcggtaccaa gcttggaggc tcaggaggct
      961 ccacaagttt gtacaaaaaa gcaggcttca tggtgagcaa gggcgaggag gataacatgg
     1021 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1141 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1201 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1321 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1381 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1441 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1501 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1561 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1621 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1681 tggacgagct gtacaagtcg ggacctaaga agaagcgcaa ggtgtgatga gacccagctt
     1741 tcttgtacaa agtggttgga ggctcaggag gctccccatg ggctagcact agtatctaga
     1801 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     1861 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     1921 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     1981 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     2041 tgttactaga tgcggccgca attgtggatc cgctctagag tcgacctgca ggcatgcaag
     2101 cttgcggccg cgtattctat agtgtcacct aaatagcatg gcgtaatcat ggtcatagct
     2161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2221 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgttttaat
     3301 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     3361 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     3421 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     3481 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     3541 gaatccggtg agaatggcaa tagcttatgc atttctttcc agacttgttc aacaggccag
     3601 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     3661 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     3721 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     3781 tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca
     3841 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     3901 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     3961 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     4021 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     4081 cttgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     4141 taagcagaca taagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4321 tttcgtc
//
