LOCUS       Circular        3850 bp DNA     circular SYN 18-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    379..395
                     /locus_tag="M13_F primer"
                     /label=M13_F primer
     misc_feature    405..423
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
     misc_feature    500..844
                     /locus_tag="CaMV 35S promoter"
                     /label=CaMV 35S promoter
     misc_feature    864..919
                     /locus_tag="TMV I(C)"
                     /label=TMV Omega
                     /label=TMV I(C)
     misc_feature    989
                     /locus_tag="Gene insert spot between c and t"
                     /label=Gene insert spot between c and t
     misc_feature    990..1250
                     /locus_tag="mVenus Cterminal"
                     /label=mVenus Cterminal
     misc_feature    1251
                     /locus_tag="Gene insert spot between c and t(1)"
                     /label=Gene insert spot between c and t
                     /label=Gene insert spot between c and t(1)
     misc_feature    1324..1574
                     /locus_tag="NOS terminator"
                     /label=NOS terminator
     misc_feature    1639..1657
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
     misc_feature    1675..1691
                     /locus_tag="M13 R_primer"
                     /label=M13 R_primer
     misc_feature    1699..1715
                     /locus_tag="lac operator"
                     /label=lac operator
     misc_feature    1723..1753
                     /locus_tag="lac promoter"
                     /label=lac promoter
     misc_feature    1768..1789
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     misc_feature    2077..2665
                     /locus_tag="ori"
                     /label=ori
     misc_feature    2823..3638
                     /locus_tag="KanR"
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaatattac tacaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgagct agtgtaatac gactcactat
      421 agggcgcggc cgcagaattc gagctcggta cccgggatct cgaggccaga tctcccgggc
      481 tcagaagacc gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      541 gattccattg cccagctatc tgtcacttat cgaaaggaca gtagaaaagg aaggtggctc
      601 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag
      661 tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac
      721 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      781 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
      841 gacaggcttc ttgagatcct tcaacaatta ccaacaacaa caaacaacaa acaacattac
      901 aattactatt tacaattaca gaattcgagc tcggtaccaa gcttggaggc tcaggaggct
      961 ccacaagttt gtacaaaaaa gcaggcttca tggtggacaa gcagaagaac ggcatcaagg
     1021 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1081 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1141 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1201 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tgagacccag
     1261 ctttcttgta caaagtggtt ggaggctcag gaggctcccc atgggctagc actagtatct
     1321 agaatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1381 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1441 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1501 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1561 ctatgttact agatgcggcc gcaattgtgg atccgctcta gagtcgacct gcaggcatgc
     1621 aagcttgcgg ccgcgtattc tatagtgtca cctaaatagc atggcgtaat catggtcata
     1681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtttt
     2821 aattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     2881 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     2941 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     3001 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     3061 actgaatccg gtgagaatgg caatagctta tgcatttctt tccagacttg ttcaacaggc
     3121 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     3181 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     3241 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     3301 tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca
     3361 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     3421 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     3481 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     3541 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     3601 ggccttgagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     3661 atgtaagcag acataagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3841 ccctttcgtc
//
