LOCUS       Exported                9153 bp DNA     circular SYN 27-APR-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 2, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
COMMENT     [DEFINITION]: 3rd generation, Inducible lentiviral expression, 
            TRE-gateway; 
[LOCUS]: Exported
[ACCESSION]: .
[SOURCE]: 
            1..9354
[CDS]: 780..1439
FEATURES             Location/Qualifiers
     source          1..9153
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..9354"
                     /note="color: #2f4f4f"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=central polypurine tract and central terminatio...
                     /note="label=cPPT/CTS - note=""central polypurine tract and
                     central termination  - sequence of HIV-1"" - 41..158"
                     /note="color: #dc143c; direction: RIGHT"
     promoter        212..526
                     /label=Tet-responsive promoter PTight, consisting of s...
                     /note="label=tight TRE promoter - note=""Tet-responsive 
                     promoter PTight, consisting of seven  - tet operator 
                     sequences followed by the minimal CMV  - promoter"" - 
                     212..526"
                     /note="color: #f08080; direction: RIGHT"
     protein_bind    220..238
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 220..238"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    256..274
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 256..274"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    291..309
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 291..309"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    327..345
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 327..345"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    363..381
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 363..381"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    398..416
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 398..416"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    434..452
                     /label=bacterial operator O2 for the tetR and tetA gen...
                     /note="gene=""tetO"" - label=tet operator - 
                     bound_moiety=""tetracycline repressor TetR"" - 
                     note=""bacterial operator O2 for the tetR and tetA genes"" 
                     - 434..452"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     499..523
                     /label=Human CMV promoter, forward primer_LNCX
                     /note="label=LNCX - note=""Human CMV promoter, forward 
                     primer"" - 499..523"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    547..671
                     /label=recombination site for the Gateway(R) LR reacti...
                     /note="gene=""mutant version of attR"" - label=attR1 - 
                     bound_moiety=""LR Clonase(TM)"" - note=""recombination site
                     for the Gateway(R) LR reaction"" - 547..671"
                     /note="color: #808000; direction: RIGHT"
     misc_recomb     548..671
                     /label=AttR1
                     /note="color: #dda0dd; direction: RIGHT"
     promoter        696..726
                     /label=E. coli lac promoter with an _lac UV5 promoter
                     /note="label=lac UV5 promoter - note=""E. coli lac promoter
                     with an """"up"""" mutation"" - 696..726"
                     /note="color: #f08080; direction: RIGHT"
     CDS             780..1439
                     /label=confers resistance to chloramphenicol_CmR
                     /note="codon_start=1 - gene=""cat"" - 
                     product=""chloramphenicol acetyltransferase"" - label=CmR -
                     note=""confers resistance to chloramphenicol"" - 
                     translation=""MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                      - 
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                      "
                     /note="color: #ff4500; direction: RIGHT"
     CDS             780..1436
                     /label=CmR
                     /note="color: #8fbc8f; direction: RIGHT"
     primer_bind     complement(846..865)
                     /label=Chloramphenicol resistance gene, reverse primer...
                     /note="label=CAT-R - note=""Chloramphenicol resistance 
                     gene, reverse primer"" - complement(846..865)"
                     /note="color: #808000; direction: LEFT"
     CDS             1781..2086
                     /label=Plasmids containing the ccdB gene cannot be _ccdB
                     /note="codon_start=1 - gene=""ccdB"" - product=""CcdB, a 
                     bacterial toxin that poisons DNA gyrase"" - label=ccdB - 
                     note=""Plasmids containing the ccdB gene cannot be  - 
                     propagated in standard E. coli strains."" - 
                     translation=""MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLS"
                     /note="color: #ff4500; direction: RIGHT"
     CDS             1874..2083
                     /label=ccdB
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     1965..1984
                     /label=ccdB gene, forward primer_ccdB-fwd
                     /note="label=ccdB-fwd - note=""ccdB gene, forward primer"" 
                     - 1965..1984"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    complement(2127..2251)
                     /label=recombination site for the Gateway(R) LR reacti...
                     /note="gene=""mutant version of attR"" - label=attR2 - 
                     bound_moiety=""LR Clonase(TM)"" - note=""recombination site
                     for the Gateway(R) LR reaction"" - complement(2127..2251)"
                     /note="color: #808000; direction: LEFT"
     misc_recomb     complement(2127..2250)
                     /label=AttR2
                     /note="color: #dda0dd; direction: LEFT"
     CDS             2269..2286
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             2269..2286
                     /label=6xHis
                     /note="codon_start=1 - product=""6xHis affinity tag"" - 
                     label=6xHis - translation=""HHHHHH"" - 2269..2286"
                     /note="color: #ff4500; direction: RIGHT"
     promoter        2296..2806
                     /label=human phosphoglycerate kinase 1 promoter_hPGK p...
                     /note="label=hPGK promoter - note=""human phosphoglycerate 
                     kinase 1 promoter"" - 2296..2806"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     2725..2743
                     /label=Human PGK promoter, forward primer_hPGK-F
                     /note="label=hPGK-F - note=""Human PGK promoter, forward 
                     primer"" - 2725..2743"
                     /note="color: #808000; direction: RIGHT"
     misc_feature    2816..3211
                     /label=blasticidin
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    3212..3265
                     /label=P2A
                     /note="color: #00ff00; direction: RIGHT"
     CDS             3212..3265
                     /label=Eukaryotic ribosomes fail to insert a peptide b...
                     /note="codon_start=1 - product=""2A peptide from Thosea 
                     asigna virus capsid  - protein"" - label=T2A - 
                     note=""Eukaryotic ribosomes fail to insert a peptide bond  
                     - between the Gly and Pro residues, yielding separate  - 
                     polypeptides."" - translation=""EGRGSLLTCGDVEENP"
                     /note="color: #ff4500; direction: RIGHT"
     CDS             3266..4012
                     /label=In the Tet-On(R) system, rtTA-Advanced binds to...
                     /note="codon_start=1 - product=""improved 
                     tetracycline-controlled transactivator"" - 
                     label=rtTA-Advanced - note=""In the Tet-On(R) system, 
                     rtTA-Advanced binds to the  - Tet-responsive element and 
                     stimulates transcription only in - the presence of 
                     tetracycline or do"
                     /note="color: #ff4500; direction: RIGHT"
     primer_bind     complement(4052..4072)
                     /label=WPRE, reverse primer_WPRE-R
                     /note="label=WPRE-R - note=""WPRE, reverse primer"" - 
                     complement(4253..4273)"
                     /note="color: #808000; direction: LEFT"
     CDS             complement(4470..4481)
                     /label=FactorXa site
                     /note="color: #008000; direction: LEFT"
     CDS             complement(4470..4481)
                     /label=Factor Xa site
                     /note="codon_start=1 - product=""Factor Xa recognition and 
                     cleavage site"" - label=Factor Xa site - 
                     translation=""IEGR"" - complement(4671..4682)"
                     /note="color: #ff4500; direction: LEFT"
     LTR             4686..4919
                     /label=self-inactivating 3' long terminal repeat (LTR)...
                     /note="label=3' LTR (Delta-U3) - note=""self-inactivating 
                     3' long terminal repeat (LTR) from - HIV-1"" - 4887..5120"
                     /note="color: #008b8b; direction: RIGHT"
     misc_feature    4686..4738
                     /label=delta U3
                     /note="color: #4682b4; direction: RIGHT"
     LTR             4739..4919
                     /label=HIV-1 5 LTR
                     /note="color: #ffa500; direction: RIGHT"
     polyA_signal    4991..5112
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /note="label=SV40 poly(A) signal - note=""SV40 
                     polyadenylation signal"" - 5192..5313"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     complement(5028..5047)
                     /label=SV40 polyA, reverse primer_SV40pA-R
                     /note="label=SV40pA-R - note=""SV40 polyA, reverse primer""
                     - complement(5229..5248)"
                     /note="color: #808000; direction: LEFT"
     primer_bind     5082..5101
                     /label=SV40 polyA terminator, reverse primer_EBV-rev
                     /note="label=EBV-rev - note=""SV40 polyA terminator, 
                     reverse primer"" - 5283..5302"
                     /note="color: #808000; direction: RIGHT"
     rep_origin      5152..5287
                     /direction=RIGHT
                     /label=SV40 origin of replication_SV40 ori
                     /note="label=SV40 ori - note=""SV40 origin of replication""
                     - 5353..5488"
                     /note="color: #f08080"
     misc_feature    5152..5229
                     /label=SV40 ORI
                     /note="color: #4682b4; direction: RIGHT"
     primer_bind     5214..5233
                     /label=SV40 promoter/origin, forward primer_SV40pro-F
                     /note="label=SV40pro-F - note=""SV40 promoter/origin, 
                     forward primer"" - 5415..5434"
                     /note="color: #808000; direction: RIGHT"
     promoter        5287..5358
                     /label=AmpR promoter
                     /note="gene=""bla"" - label=AmpR promoter - 5488..5559"
                     /note="color: #f08080; direction: RIGHT"
     promoter        5289..5317
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             5359..6219
                     /label=confers resistance to ampicillin, carbenicillin...
                     /note="codon_start=1 - gene=""bla"" - 
                     product=""beta-lactamase"" - label=AmpR - note=""confers 
                     resistance to ampicillin, carbenicillin, and - related 
                     antibiotics"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                      - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQ"
                     /note="color: #ff4500; direction: RIGHT"
     CDS             5557..6216
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     primer_bind     complement(5577..5596)
                     /label=Ampicillin resistance gene, reverse primer_Amp-R
                     /note="label=Amp-R - note=""Ampicillin resistance gene, 
                     reverse primer"" - complement(5778..5797)"
                     /note="color: #808000; direction: LEFT"
     rep_origin      6368..6996
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     rep_origin      6390..6978
                     /direction=RIGHT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o...
                     /note="direction=RIGHT - label=ori - 
                     note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of  - 
                     replication"" - 6591..7179"
                     /note="color: #f08080"
     primer_bind     6879..6898
                     /label=pBR322 origin, forward primer_pBR322ori-F
                     /note="label=pBR322ori-F - note=""pBR322 origin, forward 
                     primer"" - 7080..7099"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     7132..7149
                     /label=L4440 vector, forward primer_L4440
                     /note="label=L4440 - note=""L4440 vector, forward primer"" 
                     - 7333..7350"
                     /note="color: #808000; direction: RIGHT"
     protein_bind    7266..7287
                     /label=CAP binding activates transcription in the pres...
                     /note="label=CAP binding site - bound_moiety=""E. coli 
                     catabolite activator protein"" - note=""CAP binding 
                     activates transcription in the presence  - of cAMP."" - 
                     7467..7488"
                     /note="color: #808000; direction: RIGHT"
     promoter        7302..7332
                     /label=promoter for the E. coli lac operon_lac promoter
                     /note="label=lac promoter - note=""promoter for the E. coli
                     lac operon"" - 7503..7533"
                     /note="color: #f08080; direction: RIGHT"
     promoter        7302..7331
                     /label=lac
                     /note="color: #4682b4; direction: RIGHT"
     misc_binding    7336..7358
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     protein_bind    7340..7356
                     /label=The lac repressor binds to the lac operator to ...
                     /note="label=lac operator - bound_moiety=""lac repressor 
                     encoded by lacI"" - note=""The lac repressor binds to the 
                     lac operator to  - inhibit transcription in E. coli. This 
                     inhibition can be  - relieved by adding lactose or  - 
                     isopropyl-beta-D-thiogalactopyranoside"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     7345..7367
                     /label=In lacZ gene_M13/pUC Reverse
                     /note="label=M13/pUC Reverse - note=""In lacZ gene"" - 
                     7546..7568"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     7364..7384
                     /label=M13-rev
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     7364..7380
                     /label=In lacZ gene. Also called M13-rev_M13 Reverse
                     /note="label=M13 Reverse - note=""In lacZ gene. Also called
                     M13-rev"" - 7565..7581"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     7364..7380
                     /label=common sequencing primer, one of multiple simil...
                     /note="label=M13 rev - note=""common sequencing primer, one
                     of multiple similar  - variants"" - 7565..7581"
                     /note="color: #808000; direction: RIGHT"
     primer_bind     7399..7419
                     /label=T3 promoter, forward primer_T3
                     /note="label=T3 - note=""T3 promoter, forward primer"" - 
                     7600..7620"
                     /note="color: #808000; direction: RIGHT"
     promoter        7401..7419
                     /label=promoter for bacteriophage T3 RNA polymerase_T3...
                     /note="label=T3 promoter - note=""promoter for 
                     bacteriophage T3 RNA polymerase"" - 7602..7620"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     7402..7421
                     /label=T3
                     /note="color: #008b8b; direction: RIGHT"
     promoter        7447..7673
                     /label=Rous sarcoma virus enhancer/promoter_RSV promoter
                     /note="label=RSV promoter - note=""Rous sarcoma virus 
                     enhancer/promoter"" - 7648..7874"
                     /note="color: #f08080; direction: RIGHT"
     LTR             7674..7854
                     /label=HIV-1 5 LTR
                     /note="color: #ffa500; direction: RIGHT"
     LTR             7674..7854
                     /label=truncated 5' long terminal repeat (LTR) from HI...
                     /note="label=5' LTR (truncated) - note=""truncated 5' long 
                     terminal repeat (LTR) from HIV-1"" - 7875..8055"
                     /note="color: #008b8b; direction: RIGHT"
     misc_feature    7901..8026
                     /label=packaging signal of human immunodeficiency viru...
                     /note="label=HIV-1 Psi - note=""packaging signal of human 
                     immunodeficiency virus  - type 1"" - 8102..8227"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    7965..8009
                     /label=HIV-1 psi pack
                     /note="color: #ffa500; direction: RIGHT"
     misc_feature    8519..8752
                     /label=RRE
                     /note="color: #4682b4; direction: RIGHT"
     misc_feature    8519..8752
                     /label=The Rev response element (RRE) of HIV-1 allows ...
                     /note="label=RRE - note=""The Rev response element (RRE) of
                     HIV-1 allows for  - Rev-dependent mRNA export from the 
                     nucleus to the  - cytoplasm."" - 8720..8953"
                     /note="color: #dc143c; direction: RIGHT"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
      601 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
      661 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
      721 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
      781 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      841 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      901 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      961 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1021 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1081 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1141 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1201 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1261 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1321 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1381 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     1441 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     1501 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     1561 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     1621 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     1681 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     1741 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     1801 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     1861 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     1921 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     1981 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2041 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2101 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     2161 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2221 tttctcgttc agctttcttg tacaaagtgg tttagtaatg aaccggtcca ccaccaccac
     2281 caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     2341 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     2401 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     2461 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     2521 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     2581 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     2641 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     2701 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     2761 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatggc
     2821 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     2881 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     2941 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     3001 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     3061 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     3121 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     3181 attgctgccc tctggttatg tgtgggaggg cgaaggtaga ggttctctcc tcacttgtgg
     3241 tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg
     3301 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     3361 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     3421 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     3481 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     3541 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     3601 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     3661 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     3721 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     3781 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     3841 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     3901 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     3961 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     4021 ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4381 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4561 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc
     4621 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4681 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4921 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4981 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5101 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7081 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7141 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7201 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7261 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7321 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7381 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7441 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7501 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7561 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7621 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7681 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7741 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7801 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7861 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7921 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7981 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8041 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8101 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8161 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8221 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8281 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8341 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8401 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8461 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8521 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8581 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8641 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8701 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8761 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8821 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8881 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8941 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9001 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9061 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9121 cgtttcagac ccacctccca accccgaggg gac
//
