LOCUS       pLIX_V5_APEX_FLAG_C    9968 bp      DNA     circular   28.01.19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2316..3062
                     /label=APEX2
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3063..3101
                     /label=V5mod
                     /SerialCloner_Color=&h107F40
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="GPIPNPLLGLDST" \ 
     CDS             2262..2285
                     /label=Flag tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5285..5296)
                     /label=FactorXa site
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     8217..8236
                     /label=T3
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     8179..8199
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      7183..7811
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    8151..8173
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             6372..7031
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             780..1436
                     /label=CmR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        6104..6132
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        8117..8146
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     LTR             5554..5734
                     /label=HIV-1 5 LTR
                     /SerialCloner_Color=&hFFA500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     LTR             8489..8669
                     /label=HIV-1 5 LTR
                     /SerialCloner_Color=&hFFA500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    8780..8824
                     /label=HIV-1 psi pack
                     /SerialCloner_Color=&hFFA500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    9334..9567
                     /label=RRE
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    5501..5553
                     /label=delta U3
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    5967..6044
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(2127..2250)
                     /label=AttR2
                     /SerialCloner_Color=&hDDA0DD
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     548..671
                     /label=AttR1
                     /SerialCloner_Color=&hDDA0DD
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1874..2083
                     /label=ccdB
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac 
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag 
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt 
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt 
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg 
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta 
      541 gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 
      601 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 
      661 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt 
      721 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa 
      781 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 
      841 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 
      901 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 
      961 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 
     1021 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 
     1081 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 
     1141 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 
     1201 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 
     1261 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 
     1321 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg 
     1381 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa 
     1441 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt 
     1501 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat 
     1561 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat 
     1621 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt 
     1681 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa 
     1741 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac 
     1801 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac 
     1861 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc 
     1921 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga 
     1981 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga 
     2041 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct 
     2101 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt 
     2161 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 
     2221 tttctcgttc agctttcttg tacaaagtgg ttgattctag agactacaaa gacgatgacg 
     2281 ataaaccagc gggcggcggc cccggctctg gagggggaaa gtcttaccca actgtgagtg 
     2341 ctgattacca ggacgccgtt gagaaggcga agaagaagct cagaggcttc atcgctgaga 
     2401 agagatgcgc tcctctaatg ctccgtttgg cattccactc tgctggaacc tttgacaagg 
     2461 gcacgaagac cggtggaccc ttcggaacca tcaagcaccc tgccgaactg gctcacagcg 
     2521 ctaacaacgg tcttgacatc gctgttaggc ttttggagcc actcaaggcg gagttcccta 
     2581 ttttgagcta cgccgatttc taccagttgg ctggcgttgt tgccgttgag gtcacgggtg 
     2641 gacctaaggt tccattccac cctggaagag aggacaagcc tgagccacca ccagagggtc 
     2701 gcttgcctga tcccactaag ggttctgacc atttgagaga tgtgtttggc aaagctatgg 
     2761 ggcttactga ccaagatatc gttgctctat ctgggggtca cactattgga gctgcacaca 
     2821 aggagcgttc tggatttgag ggtccctgga cctctaatcc tcttattttc gacaactcat 
     2881 acttcacgga gttgttgagt ggtgagaagg aaggtctcct tcagctacct tctgacaagg 
     2941 ctcttttgtc tgaccctgta ttccgccctc tcgttgataa atatgcagcg gacgaagatg 
     3001 ccttctttgc tgattacgct gaggctcacc aaaagctttc cgagcttggg tttgctgatg 
     3061 ccggacccat ccccaacccc ctgctgggcc tggacagcac ctaaggatcc ggggttgggg 
     3121 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt 
     3181 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc 
     3241 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc 
     3301 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca 
     3361 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg 
     3421 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa 
     3481 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg 
     3541 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga 
     3601 atcaccgacc tctctcccca gcaattcacc Atggccaagc ctttgtctca agaagaatcc 
     3661 accctcattg aaagagcaac ggctacaatc aacaGcatcc ccatctctga agactacagc 
     3721 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtaTatcat 
     3781 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct 
     3841 ggcaacctga cttgTatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc 
     3901 ggacggtgcc gacaggtgct tctcgatctg catcCtggga tcaaagccat agtgaaggac 
     3961 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatGtgtgg 
     4021 gagggcgaag gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca 
     4081 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc 
     4141 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc 
     4201 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg 
     4261 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg 
     4321 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat 
     4381 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg 
     4441 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt 
     4501 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca 
     4561 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag 
     4621 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag 
     4681 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc 
     4741 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat 
     4801 tttgaccttg acatgctccc cgggtaacta agtaaggatc gatccaagat atcgtattct 
     4861 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 
     4921 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 
     4981 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 
     5041 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 
     5101 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     5161 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 
     5221 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 
     5281 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 
     5341 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 
     5401 gcctgtttcg cctcggcgtc cggactagag gtacctttaa gaccaatgac ttacaaggca 
     5461 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     5521 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     5581 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     5641 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     5701 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     5761 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     5821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     5881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     5941 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     6001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     6061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctttcaaat atgtatccgc 
     6121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 
     6181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 
     6241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 
     6301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 
     6361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 
     6421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 
     6481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 
     6541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 
     6601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 
     6661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 
     6721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 
     6781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 
     6841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 
     6901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 
     6961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 
     7021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 
     7081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     7141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     7201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     7261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     7321 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 
     7381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     7441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     7501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     7561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     7621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     7681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     7741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     7801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     7861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 
     7921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 
     7981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 
     8041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 
     8101 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 
     8161 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta 
     8221 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact 
     8281 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa 
     8341 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca 
     8401 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt 
     8461 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg 
     8521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     8581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     8641 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa 
     8701 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga 
     8761 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag 
     8821 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa 
     8881 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc 
     8941 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga 
     9001 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta 
     9061 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag 
     9121 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg 
     9181 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata 
     9241 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag 
     9301 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg 
     9361 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt 
     9421 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca 
     9481 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga 
     9541 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg 
     9601 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa 
     9661 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc 
     9721 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga 
     9781 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt 
     9841 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat 
     9901 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc 
     9961 gaggggac
//