LOCUS       Exported                9265 bp ds-DNA     circular SYN 23-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2019 from SnapGene Viewer 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    543..659
                     /label=change N
     misc_feature    551..592
                     /label=V5mod [*]
                     /note="note=""direct strand translation"" 
                     translation=""MGPIPNPLLGLDSTASKDNTVPLKLIALLANGEFHSGEQLGETLG
                     MSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDS
                     TNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGL
                     GPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGIT"
     CDS             623..646
                     /label=Flag tag
     misc_recomb     660..783
                     /label=AttR1
     CDS             892..1548
                     /label=CmR
     CDS             1986..2195
                     /label=ccdB
     misc_recomb     complement(2239..2362)
                     /label=AttR2
     CDS             2381..2398
                     /label=6His
     misc_feature    2928..3323
                     /label=Blasti
     CDS             complement(4582..4593)
                     /label=FactorXa site
     misc_feature    4798..4850
                     /label=delta U3
     LTR             4851..5031
                     /label=HIV-1 5 LTR
     misc_feature    5264..5341
                     /label=SV40 ORI
     promoter        5401..5429
                     /label=Amp prom
     CDS             5669..6328
                     /label=AmpR
     rep_origin      6480..7108
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        7414..7443
                     /label=lac
     misc_binding    7448..7470
                     /label=LacO
     primer_bind     7476..7496
                     /label=M13-rev
     primer_bind     7514..7533
                     /label=T3
     LTR             7786..7966
                     /label=HIV-1 5 LTR
     misc_feature    8077..8121
                     /label=HIV-1 psi pack
     misc_feature    8631..8864
                     /label=RRE
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaaacc atgggaccca tccccaaccc cctgctgggc ctggacagca ccgggggatc
      601 tggccccggc ggcggcgcgc cagactacaa agacgatgac gataaactag gcgcgcccac
      661 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      721 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      781 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      841 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
      901 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      961 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1021 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1081 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1141 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1201 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1261 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1321 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1381 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1441 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1501 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga
     1561 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     1621 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     1681 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     1741 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     1801 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     1861 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     1921 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     1981 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2041 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2101 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2161 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2221 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2281 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     2341 tcagctttct tgtacaaagt ggtttagtaa tgaaccggtc caccaccacc accaccacta
     2401 aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     2461 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     2521 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     2581 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     2641 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     2701 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     2761 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     2821 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     2881 gctccctcgt tgaccgaatc accgacctct ctccccagca attcaccatg gccaagcctt
     2941 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     3001 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     3061 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     3121 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     3181 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     3241 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     3301 cctctggtta tgtgtgggag ggcgaaggta gaggttctct cctcacttgt ggtgatgttg
     3361 aagaaaaccc tggtccaatg tctagactgg acaagagcaa agtcataaac ggagctctgg
     3421 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     3481 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     3541 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
     3601 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     3661 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     3721 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     3781 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     3841 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
     3901 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
     3961 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
     4021 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     4081 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat
     4141 ccaagatatc gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4201 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4261 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4321 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4381 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4441 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4501 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4561 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4621 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4681 ctttgggccg cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac
     4741 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4801 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4861 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4921 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4981 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5041 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5101 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5161 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5221 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     5281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7261 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7381 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7441 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7501 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7561 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     7621 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     7681 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     7741 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     7801 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7861 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7921 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7981 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8041 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     8101 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     8161 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     8221 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     8281 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     8341 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     8401 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8461 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8521 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8581 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8641 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8701 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8761 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8821 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8881 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8941 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9001 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9061 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9121 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     9181 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     9241 acccacctcc caaccccgag gggac
//
