LOCUS       pBB75-ncRNA             5180 bp    DNA     circular UNA 03-NOV-2022
DEFINITION  8 reads from 8 documents assembled using Geneious.
ACCESSION   urn.local...s-fi2n7bh
VERSION     urn.local...s-fi2n7bh
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    554..1044
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="Tn3R reg"
                     /X-Transferred_Similarity="67.21%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="Tn3R reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    808..814
                     /Recognition_pattern="G^GTNACC"
                     /Methylation="Dcm:cut, CpG:cut, EcoBI:impaired,
                     EcoKI:impaired"
                     /annotation_group="GGTNACC"
                     /label="BstEII"
                     /note="Geneious type: restriction site"
     misc_feature    1075..1080
                     /Recognition_pattern="GAT^ATC"
                     /Methylation="CpG:some impaired, EcoBI:some blocked"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    1131..1136
                     /Recognition_pattern="GTT^AAC"
                     /Methylation="CpG:variable, EcoKI:variable"
                     /annotation_group="GTTAAC"
                     /label="HpaI"
                     /note="Geneious type: restriction site"
     misc_feature    1131..1136
                     /Recognition_pattern="GTY^RAC"
                     /Methylation="CpG:variable, EcoBI:blocked, EcoKI:blocked"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     Promoter        1408..1437
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="lac prom"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     Reporter        1499..1659
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="lacZ_a reporter"
                     /X-Transferred_Similarity="99.38%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="lacZ_a reporter"
     misc_feature    1946..1951
                     /Recognition_pattern="G^TGCAC"
                     /Methylation="CpG:blocked, EcoKI:variable"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     Promoter        2010..2028
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7 prom"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     Gene            2065..2081
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    2087..2092
                     /Recognition_pattern="CA^TATG"
                     /Methylation="EcoBI:cut"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     ncRNA           2093..2282
                     /created_by="chen"
                     /label="Ec86-ncRNA"
     misc_feature    2286..2291
                     /Recognition_pattern="G^AATTC"
                     /Methylation="CpG:variable, EcoBI:variable"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    2292..2297
                     /Recognition_pattern="GAGCT^C"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     terminator      2316..2438
                     /note="T7 Tphi transcription terminator"
                     /gene="expression_cassette"
                     /X-Transferred_From="pET11c; pTWIN1; pTXB1; pTYB1; pTYB11"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="T7 Tphi transcription terminator; expression
                     cassette terminator"
     Terminator      2377..2420
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7 term"
                     /X-Transferred_Similarity="97.73%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="T7 term"
     misc_feature    2571..2576
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     rep_origin      3055..3600
                     /note="p15A origin of replication (clockwise) (RNAII -35
                     to RNA/DNA switch point)"
                     /X-Transferred_From="pACYC177; pACYC184"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="rep origin"
     misc_feature    3849..3854
                     /Recognition_pattern="GT^MKAC"
                     /Methylation="CpG:blocked"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    3908..3913
                     /Recognition_pattern="AGG^CCT"
                     /Methylation="Dcm:blocked, EcoBI:cut"
                     /annotation_group="AGGCCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     CDS             4195..5010
                     /gene="aph(3')-Ia"
                     /note="kanR (confers resistance to kanamycin)"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from Tn903"
                     /X-Transferred_Translation="MSHIQRETSCSRPRLNSNMDADLYGYKWAR
                     DNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFI
                     RTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRV
                     FRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNL
                     IFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQ
                     FHLMLDEFF"
                     /X-Transferred_From="pACYC177; pGPS1.1; pGPS3; pGPS5"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="aph(3')-Ia CDS"
     Marker          4195..5010
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="kan2 marker"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="kan2 marker"
     gene            4195..5010
                     /gene="aph(3')-Ia"
                     /X-Transferred_From="pACYC177; pGPS1.1; pGPS3; pGPS5"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Consensus, Consensus extraction"
                     /Transferred_Similarity="100.00%"
                     /label="aph(3')-Ia gene"
     misc_feature    4224..4229
                     /Recognition_pattern="C^TCGAG"
                     /Methylation="CpG:impaired"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    4281..4286
                     /Recognition_pattern="TCG^CGA"
                     /Methylation="Dam:blocked, CpG:blocked"
                     /annotation_group="TCGCGA"
                     /label="NruI"
                     /note="Geneious type: restriction site"
     misc_feature    4316..4321
                     /Recognition_pattern="AT^CGAT"
                     /Methylation="Dam:blocked, CpG:blocked, EcoBI:variable"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     misc_feature    4498..4503
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="CpG:some impaired"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4498..4503
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4744..4749
                     /Recognition_pattern="A^AGCTT"
                     /Methylation="EcoBI:some blocked"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cntntngana nngncccttc
      121 gggcgaanta tttatttccn ngtcnattnn acagaaancg ggnttttttc caaaaaatna
      181 ngggtaatgn attaatccct ggataangaa taaaanttgc caagnntcat ttgatgctcn
      241 tatgaagttt tttctaaatc agaaagtggt naaatnggtn agtaacanna ggcagancat
      301 tacnntgact tracgggacn ggcgggntgt gatngaataa atcgaacttc ttncngagtn
      361 gaaggatcag atcacgcatc ttccccgaca acncagncng ttcngtggca aagcaaaagt
      421 tnaaaatcan caactggtcc acctncanca aagctctcat caaccgtgnc tccctcactt
      481 tctgnctgga tgatggggcg atncaggnnn gntatgagtc agcaacacct tcttcacgag
      541 gcagacctca gcgctcaaag atgcaggggt aaaagctaac cgcatcttta ccgacaaggc
      601 atccggcagt tcaacagatc gggaagggct ggatttgctg aggatgaagg tggaggaagg
      661 tgatgtcatt ctggtgaaga agctcgaccg tcttggccgc gacaccgccg acatgatcca
      721 actgataaaa gagtttgatg ctcagggtgt agcggttcgg tttattgacg acgggatcag
      781 taccgacggt gatatggggc aaatggtggt caccagcaaa tcgcgctgtt agcgggccca
      841 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
      901 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
      961 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     1021 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     1081 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     1141 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1201 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1261 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1321 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag
     1381 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1441 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt
     1501 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1561 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1621 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
     1681 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
     1741 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
     1801 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
     1861 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
     1921 ttggaattag cttatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
     1981 aagctgtggt gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
     2041 gtttccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgtgataaga
     2101 ttccgtatgc gcacccttag cgagaggttt atcattaagg tcaacctctg gatgttgttt
     2161 cggcatcctg cattgaatct gagttactgt ctgttttcct tgttggaacg gagagcatcg
     2221 cctgatgctc tccgagccaa ccaggaaacc cgttttttct gacgtaaggg tgcgcaactt
     2281 tctaagaatt cgagctctag ataagtaaga tccggctgct aacaaagccc gaaaggaagc
     2341 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     2401 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatagtcac gtagcgatag
     2461 cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2521 tgtcatgata ataatggttt cttagccgat gcccttgaga gccttcaacc ctgcagcaat
     2581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3001 ttaacgtgag ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga
     3061 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg
     3121 tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     3181 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa
     3241 ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt
     3301 tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     3361 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     3421 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     3481 cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3541 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     3601 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     3661 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     3721 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     3781 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     3841 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac
     3901 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     3961 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     4021 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     4081 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     4141 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     4201 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
     4261 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
     4321 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     4381 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     4441 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     4501 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     4561 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     4621 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     4681 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     4741 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     4801 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     4861 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     4921 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     4981 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     5041 cagagcatta cgctgacttg acgggacggc gggctttgtt gaattnaatc nnacttttgc
     5101 tgagctgaan gatcnnatna ccnatcttcc ccnacaccnc agaccnntcc ggnggcaang
     5161 nnanntttaa aatcncanct
//
