LOCUS       Pramr-GFP               4473 bp ds-DNA     circular     04-NOV-2022
DEFINITION  .
ACCESSION   urn.local...1367864416015.9
VERSION     urn.local...1367864416015.9
COMMENT     Imported using the Genbank importer. File name: p15A-LcI-KODexo
            92-174AAm86-DrepA TetO.gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(163..1122)
                     /label="LacI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1405..1438
                     /label="Bidirectional Term"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1470..1536
                     /label="RamR-promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1563..2309
                     /label="GFPmut2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      2313..2360
                     /label="LuxI term"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(2581..3240)
                     /label="CAT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3599..4438
                     /label="p15A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc
       61 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
      121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
      181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
      301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
      361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
      421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
      481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
      541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
      601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
      661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
      721 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
      781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
      841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
      901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
      961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     1021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     1081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     1141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     1201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     1261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     1321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta taaacgcagg
     1381 tcttttcgcc ggtgtatttc ttgcaaaaaa aaaccccgcc gaagcggggt tttttttttt
     1441 acagggctat aaggtagatc cgggtcgacC TTTGAAAAGT ACCttgacaG CGTATCTTTG
     1501 CTTTCtataa tGAGTGCTTA CTCACTCATA ATCAAGtact agagaaagag gagaaatact
     1561 agatggggat gaccatgatt acgccaagct ttatgagtaa aggagaagaa cttttcactg
     1621 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1681 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1741 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcgcgtat ggtcttcaat
     1801 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1861 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1921 ctgaagtcaa atttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1981 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     2041 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     2101 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     2161 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     2221 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     2281 cacatggcat ggatgaacta tacaaataat aaagagagaa tataaaaagc cagattatta
     2341 atccggcttt tttattattt ctgaaacctc aggcatttga gaagcacacg gtcacactgc
     2401 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
     2461 gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
     2521 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
     2581 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     2641 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     2701 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc
     2761 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     2821 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     2881 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     2941 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     3001 accgtctttc attgccatac ggaactccgg atgagcattc atcaggcggg caagaatgtg
     3061 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     3121 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     3181 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     3241 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     3301 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcacgtctca ttttcgccaa
     3361 agttggccag ggcttcccgg tatcaacagg gacaccagga tttatttatn ntgcgaagtg
     3421 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact
     3481 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     3541 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
     3601 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
     3661 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
     3721 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     3781 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     3841 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     3901 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     3961 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     4021 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     4081 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     4141 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     4201 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     4261 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     4321 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     4381 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     4441 tttctagatt tcagtgcaat ttatctcttc aaa
//