LOCUS       pYDE009(pUC18TminiTn7TGm-dCas9)      9673 bp    DNA     circular SYN 25-MAY-2010
DEFINITION  Cloning vector pUC18T-mini-Tn7T-Gm, complete sequence.
ACCESSION   AY599232
VERSION     AY599232.2  GI:296840611
KEYWORDS    .
SOURCE      Cloning vector pUC18T-mini-Tn7T-Gm (unknown)
  ORGANISM  Cloning vector pUC18T-mini-Tn7T-Gm
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Choi,K.H., Gaynor,J.B., White,K.G., Lopez,C., Bosio,C.M.,
            Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
   PUBMED   15908923
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Choi,K.H., Gaynor,J., Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2004) Microbiology, Immunology and Pathology,
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 4569)
  AUTHORS   Choi,K.-H. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Microbiology, Immunology and Pathology,
            Colorado State University, 1682 Campus delivery, Ft. Collins, CO
            80523, USA
   REMARK   Sequence update by submitter
COMMENT     On May 25, 2010 this sequence version replaced gi:47132196.
FEATURES             Location/Qualifiers
     Vector          join(7139..9673,1..2012)
                     /origin="pUC18T-mini-Tn7T-Gm-AY599232 (4,569bp
                     circular)Target vector: from PstI cut at 2034^2035 to
                     SpeI cut at 2012^2013, 4,547bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
     source          join(<7139..9673,1..>2012)
                     /organism="Cloning vector pUC18T-mini-Tn7T-Gm"
                     /mol_type="other DNA"
                     /db_xref="taxon:275444"
     mobile_element  382..580
                     /note="right end of Tn7"
                     /mobile_element_type="transposon:Tn7R"
     terminator      629..723
                     /note="bacteriophage lambda T0"
     terminator      820..917
                     /note="Escherichia coli rrnB T1"
     misc_feature    958..1005
                     /note="FRT; Flp recombinase target site"
     CDS             complement(1135..1668)
                     /gene="aacC1"
                     /codon_start=1
                     /transl_table=11
                     /product="gentamycin acetyl transferase"
                     /protein_id="AAT11770.1"
                     /db_xref="GI:47132197"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
     gene            complement(1135..1668)
                     /gene="aacC1"
     misc_feature    1926..1973
                     /note="FRT; Flp recombinase target site"
     misc_feature    join(<7139..7181,2000..>2012)
                     /note="MCS; multiple cloning site"
     misc_feature    2013..7138
                     /feature_type="Insertion"
                     /origin="pUC18-dCas9 Sequencing (7,596bp linear)Fragment:
                     from SpeI cut at 198^199 to PstI cut at 5324^5325,
                     5,126bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     mobile_element  7190..7355
                     /note="left end of Tn7"
                     /mobile_element_type="transposon:Tn7L"
     misc_feature    7507..7774
                     /note="oriT; origin of transfer"
     CDS             complement(8613..9473)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAT11771.1"
                     /db_xref="GI:47132198"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(8613..9473)
                     /gene="bla"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT AGAGGACCAG CCGCGTAACC TGGCAAAATC GGTTACGGTT GAGTAATAAA
      361 TGGATGCCCT GCGTAAGCGG GTGTGGGCGG ACAATAAAGT CTTAAACTGA ACAAAATAGA
      421 TCTAAACTAT GACAATAAAG TCTTAAACTA GACAGAATAG TTGTAAACTG AAATCAGTCC
      481 AGTTATGCTG TGAAAAAGCA TACTGGACTT TTGTTATGGC TAAAGCAAAC TCTTCATTTT
      541 CTGAAGTGCA AATTGCCCGT CGTATTAAAG AGGGGCGTGG GGTTCGAGGT CGACGGTATC
      601 GATAAGCTAG CTTAATTAGC TGAGCTTGGA CTCCTGTTGA TAGATCCAGT AATGACCTCA
      661 GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATTGGTGAG
      721 AATCCAAGCT AGACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT
      781 GGTGCCCTTA AACGCCTGGG GTAATGACTC TCTAGCTTGA GGCATCAAAT AAAACGAAAG
      841 GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG
      901 AGTAGGACAA ATCCGCCGCT AGGAGCTTGC GGCCCGGACG ATCGAATTGG GGATCTTGAA
      961 GTTCCTATTC CGAAGTTCCT ATTCTCTAGA AAGTATAGGA ACTTCAGAGC GCTTTTGAAG
     1021 CTGATGTGCT TAAAAACTTA CTCAATGGAA TTCTAGATCG AATTGGCCGC GGCGTTGTGA
     1081 CAATTTACCG AACAACTCCG CGGCCGGGAA GCCGATCTCG GCTTGAACGA ATTGTTAGGT
     1141 GGCGGTACTT GGGTCGATAT CAAAGTGCAT CACTTCTTCC CGTATGCCCA ACTTTGTATA
     1201 GAGAGCCACT GCGGGATCGT CACCGTAATC TGCTTGCACG TAGATCACAT AAGCACCAAG
     1261 CGCGTTGGCC TCATGCTTGA GGAGATTGAT GAGCGCGGTG GCAATGCCCT GCCTCCGGTG
     1321 CTCGCCGGAG ACTGCGAGAT CATAGATATA GATCTCACTA CGCGGCTGCT CAAACCTGGG
     1381 CAGAACGTAA GCCGCGAGAG CGCCAACAAC CGCTTCTTGG TCGAAGGCAG CAAGCGCGAT
     1441 GAATGTCTTA CTACGGAGCA AGTTCCCGAG GTAATCGGAG TCCGGCTGAT GTTGGGAGTA
     1501 GGTGGCTACG TCTCCGAACT CACGACCGAA AAGATCAAGA GCAGCCCGCA TGGATTTGAC
     1561 TTGGTCAGGG CCGAGCCTAC ATGTGCGAAT GATGCCCATA CTTGAGCCAC CTAACTTTGT
     1621 TTTAGGGCGA CTGCCCTGCT GCGTAACATC GTTGCTGCTG CGTAACATCG TTGCTGCTCC
     1681 ATAACATCAA ACATCGACCC ACGGCGTAAC GCGCTTGCTG CTTGGATGCC CGAGGCATAG
     1741 ACTGTACAAA AAAACAGTCA TAACAAGCCA TGAAAACCGC CACTGCGCCG TTACCACCGC
     1801 TGCGTTCGGT CAAGGTTCTG GACCAGTTGC GTGAGCGCAT ACGCTACTTG CATTACAGTT
     1861 TACGAACCGA ACAGGCTTAT GTCAATTCGA TCCATTGCTG TTGACAAAGG GAATCAGGGG
     1921 ATCTTGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCAGAGCGC
     1981 TTTTGAAGCT AATTCGATCA TGCATGAGCT CACTAGttta agacccactt tcacatttaa
     2041 gTTGTTTTTC TAATCCGCAT ATGATCAATT CAAGGCCGAA TAAGAAGGCT GGCTCTGCAC
     2101 CTTGGTGATC AAATAATTCG ATAGCTTGTC GTAATAATGG CGGCATACTA TCAGTAGTAG
     2161 GTGTTTCCCT TTCTTCTTTA GCGACTTGAT GCTCTTGATC TTCCAATACG CAACCTAAAG
     2221 TAAAATGCCC CACAGCGCTG AGTGCATATA ATGCATTCTC TAGTGAAAAA CCTTGTTGGC
     2281 ATAAAAAGGC TAATTGATTT TCGAGAGTTT CATACTGTTT TTCTGTAGGC CGTGTACCTA
     2341 AATGTACTTT TGCTCCATCG CGATGACTTA GTAAAGCACA TCTAAAACTT TTAGCGTTAT
     2401 TACGTAAAAA ATCTTGCCAG CTTTCCCCTT CTAAAGGGCA AAAGTGAGTA TGGTGCCTAT
     2461 CTAACATCTC AATGGCTAAG GCGTCGAGCA AAGCCCGCTT ATTTTTTACA TGCCAATACA
     2521 ATGTAGGCTG CTCTACACCT AGCTTCTGGG CGAGTTTACG GGTTGTTAAA CCTTCGATTC
     2581 CGACCTCATT AAGCAGCTCT AATGCGCTGT TAATCACTTT ACTTTTATCT AATCTAGACA
     2641 TCATTAATTC CTAATTTTTG TTGACACTCT ATCGTTGATA GAGTTATTTT ACCACTCCCT
     2701 ATCAGTGATA GAGAAAAGAA TTCAAAAGAT CTAAAGAGGA GAAAGGATCT ATGGATAAGA
     2761 AATACTCAAT AGGCTTAGCT ATCGGCACAA ATAGCGTCGG ATGGGCGGTG ATCACTGATG
     2821 AATATAAGGT TCCGTCTAAA AAGTTCAAGG TTCTGGGAAA TACAGACCGC CACAGTATCA
     2881 AAAAAAATCT TATAGGGGCT CTTTTATTTG ACAGTGGAGA GACAGCGGAA GCGACTCGTC
     2941 TCAAACGGAC AGCTCGTAGA AGGTATACAC GTCGGAAGAA TCGTATTTGT TATCTACAGG
     3001 AGATTTTTTC AAATGAGATG GCGAAAGTAG ATGATAGTTT CTTTCATCGA CTTGAAGAGT
     3061 CTTTTTTGGT GGAAGAAGAC AAGAAGCATG AACGTCATCC TATTTTTGGA AATATAGTAG
     3121 ATGAAGTTGC TTATCATGAG AAATATCCAA CTATCTATCA TCTGCGAAAA AAATTGGTAG
     3181 ATTCTACTGA TAAAGCGGAT TTGCGCTTAA TCTATTTGGC CTTAGCGCAT ATGATTAAGT
     3241 TTCGTGGTCA TTTTTTGATT GAGGGAGATT TAAATCCTGA TAATAGTGAT GTGGACAAAC
     3301 TATTTATCCA GTTGGTACAA ACCTACAATC AATTATTTGA AGAAAACCCT ATTAACGCAA
     3361 GTGGAGTAGA TGCTAAAGCG ATTCTTTCTG CACGATTGAG TAAATCAAGA CGATTAGAAA
     3421 ATCTCATTGC TCAGCTCCCC GGTGAGAAGA AAAATGGCTT ATTTGGGAAT CTCATTGCTT
     3481 TGTCATTGGG TTTGACCCCT AATTTTAAAT CAAATTTTGA TTTGGCAGAA GATGCTAAAT
     3541 TACAGCTTTC AAAAGATACT TACGATGATG ATTTAGATAA TTTATTGGCG CAAATTGGAG
     3601 ATCAATATGC TGATTTGTTT TTGGCAGCTA AGAATTTATC AGATGCTATT TTACTTTCAG
     3661 ATATCCTAAG AGTAAATACT GAAATAACTA AGGCTCCCCT ATCAGCTTCA ATGATTAAAC
     3721 GCTACGATGA ACATCATCAA GACTTGACTC TTTTAAAAGC TTTAGTTCGA CAACAACTTC
     3781 CAGAAAAGTA TAAAGAAATC TTTTTTGATC AATCAAAAAA CGGATATGCA GGTTATATTG
     3841 ATGGGGGAGC TAGCCAAGAA GAATTTTATA AATTTATCAA ACCAATTTTA GAAAAAATGG
     3901 ATGGTACTGA GGAATTATTG GTGAAACTAA ATCGTGAAGA TTTGCTGCGC AAGCAACGGA
     3961 CCTTTGACAA CGGCTCTATT CCCCATCAAA TTCACTTGGG TGAGCTGCAT GCTATTTTGA
     4021 GAAGACAAGA AGACTTTTAT CCATTTTTAA AAGACAATCG TGAGAAGATT GAAAAAATCT
     4081 TGACTTTTCG AATTCCTTAT TATGTTGGTC CATTGGCGCG TGGCAATAGT CGTTTTGCAT
     4141 GGATGACTCG GAAGTCTGAA GAAACAATTA CCCCATGGAA TTTTGAAGAA GTTGTCGATA
     4201 AAGGTGCTTC AGCTCAATCA TTTATTGAAC GCATGACAAA CTTTGATAAA AATCTTCCAA
     4261 ATGAAAAAGT ACTACCAAAA CATAGTTTGC TTTATGAGTA TTTTACGGTT TATAACGAAT
     4321 TGACAAAGGT CAAATATGTT ACTGAAGGAA TGCGAAAACC AGCATTTCTT TCAGGTGAAC
     4381 AGAAGAAAGC CATTGTTGAT TTACTCTTCA AAACAAATCG AAAAGTAACC GTTAAGCAAT
     4441 TAAAAGAAGA TTATTTCAAA AAAATAGAAT GTTTTGATAG TGTTGAAATT TCAGGAGTTG
     4501 AAGATAGATT TAATGCTTCA TTAGGTACCT ACCATGATTT GCTAAAAATT ATTAAAGATA
     4561 AAGATTTTTT GGATAATGAA GAAAATGAAG ATATCTTAGA GGATATTGTT TTAACATTGA
     4621 CCTTATTTGA AGATAGGGAG ATGATTGAGG AAAGACTTAA AACATATGCT CACCTCTTTG
     4681 ATGATAAGGT GATGAAACAG CTTAAACGTC GCCGTTATAC TGGTTGGGGA CGTTTGTCTC
     4741 GAAAATTGAT TAATGGTATT AGGGATAAGC AATCTGGCAA AACAATATTA GATTTTTTGA
     4801 AATCAGATGG TTTTGCCAAT CGCAATTTTA TGCAGCTGAT CCATGATGAT AGTTTGACAT
     4861 TTAAAGAAGA CATTCAAAAA GCACAAGTGT CTGGACAAGG CGATAGTTTA CATGAACATA
     4921 TTGCAAATTT AGCTGGTAGC CCTGCTATTA AAAAAGGTAT TTTACAGACT GTAAAAGTTG
     4981 TTGATGAATT GGTCAAAGTA ATGGGGCGGC ATAAGCCAGA AAATATCGTT ATTGAAATGG
     5041 CACGTGAAAA TCAGACAACT CAAAAGGGCC AGAAAAATTC GCGAGAGCGT ATGAAACGAA
     5101 TCGAAGAAGG TATCAAAGAA TTAGGAAGTC AGATTCTTAA AGAGCATCCT GTTGAAAATA
     5161 CTCAATTGCA AAATGAAAAG CTCTATCTCT ATTATCTCCA AAATGGAAGA GACATGTATG
     5221 TGGACCAAGA ATTAGATATT AATCGTTTAA GTGATTATGA TGTCGATGCC ATTGTTCCAC
     5281 AAAGTTTCCT TAAAGACGAT TCAATAGACA ATAAGGTCTT AACGCGTTCT GATAAAAATC
     5341 GTGGTAAATC GGATAACGTT CCAAGTGAAG AAGTAGTCAA AAAGATGAAA AACTATTGGA
     5401 GACAACTTCT AAACGCCAAG TTAATCACTC AACGTAAGTT TGATAATTTA ACGAAAGCTG
     5461 AACGTGGAGG TTTGAGTGAA CTTGATAAAG CTGGTTTTAT CAAACGCCAA TTGGTTGAAA
     5521 CTCGCCAAAT CACTAAGCAT GTGGCACAAA TTTTGGATAG TCGCATGAAT ACTAAATACG
     5581 ATGAAAATGA TAAACTTATT CGAGAGGTTA AAGTGATTAC CTTAAAATCT AAATTAGTTT
     5641 CTGACTTCCG AAAAGATTTC CAATTCTATA AAGTACGTGA GATTAACAAT TACCATCATG
     5701 CCCATGATGC GTATCTAAAT GCCGTCGTTG GAACTGCTTT GATTAAGAAA TATCCAAAAC
     5761 TTGAATCGGA GTTTGTCTAT GGTGATTATA AAGTTTATGA TGTTCGTAAA ATGATTGCTA
     5821 AGTCTGAGCA AGAAATAGGC AAAGCAACCG CAAAATATTT CTTTTACTCT AATATCATGA
     5881 ACTTCTTCAA AACAGAAATT ACACTTGCAA ATGGAGAGAT TCGCAAACGC CCTCTAATCG
     5941 AAACTAATGG GGAAACTGGA GAAATTGTCT GGGATAAAGG GCGAGATTTT GCCACAGTGC
     6001 GCAAAGTATT GTCCATGCCC CAAGTCAATA TTGTCAAGAA AACAGAAGTA CAGACAGGCG
     6061 GATTCTCCAA GGAGTCAATT TTACCAAAAA GAAATTCGGA CAAGCTTATT GCTCGTAAAA
     6121 AAGACTGGGA TCCAAAAAAA TATGGTGGTT TTGATAGTCC AACGGTAGCT TATTCAGTCC
     6181 TAGTGGTTGC TAAGGTGGAA AAAGGGAAAT CGAAGAAGTT AAAATCCGTT AAAGAGTTAC
     6241 TAGGGATCAC AATTATGGAA AGAAGTTCCT TTGAAAAAAA TCCGATTGAC TTTTTAGAAG
     6301 CTAAAGGATA TAAGGAAGTT AAAAAAGACT TAATCATTAA ACTACCTAAA TATAGTCTTT
     6361 TTGAGTTAGA AAACGGTCGT AAACGGATGC TGGCTAGTGC CGGAGAATTA CAAAAAGGAA
     6421 ATGAGCTGGC TCTGCCAAGC AAATATGTGA ATTTTTTATA TTTAGCTAGT CATTATGAAA
     6481 AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT GTTTGTGGAG CAGCATAAGC
     6541 ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC TAAGCGTGTT ATTTTAGCAG
     6601 ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA TAGAGACAAA CCAATACGTG
     6661 AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA TCTTGGAGCT CCCGCTGCTT
     6721 TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC GTCTACAAAA GAAGTTTTAG
     6781 ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA AACACGCATT GATTTGAGTC
     6841 AGCTAGGAGG TGACTAACTC GAGTAAGGAT CTCCAGGCAT CAAATAAAAC GAAAGGCTCA
     6901 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG
     6961 TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT ACCTAGGGAT ATATTCCGCT
     7021 TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC
     7081 GAACGGGGCG GAGATTTCCT GGAAGATGCc aggaagatac ttaacaggga agtcTGCAGG
     7141 AATTCCTCGA GAAGCTTGGG CCCGGTACCT CGCGAAGGCC TTGCAGGCCA ACCAGATAAG
     7201 TGAAATCTAG TTCCAAACTA TTTTGTCATT TTTAATTTTC GTATTAGCTT ACGACGCTAC
     7261 ACCCAGTTCC CATCTATTTT GTCACTCTTC CCTAAATAAT CCTTAAAAAC TCCATTTCCA
     7321 CCCCTCCCAG TTCCCAACTA TTTTGTCCGC CCACAGCGGG GCATTTTTCT TCCTGTTATG
     7381 TTTGGGCGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     7441 GCTCTTCCGC TTCCTCGCTC ACTGACCCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
     7501 TATCAGAGCT TATCGGCCAG CCTCGCAGAG CAGGATTCCC GTTGAGCACC GCCAGGTGCG
     7561 AATAAGGGAC AGTGAAGAAG GAACACCCGC TCGCGGGTGG GCCTACTTCA CCTATCCTGC
     7621 CCGGCTGACG CCGTTGGATA CACCAAGGAA AGTCTACACG AACCCTTTGG CAAAATCCTG
     7681 TATATCGTGC GAAAAAGGAT GGATATACCG AAAAAATCGC TATAATGACC CCGAAGCAGG
     7741 GTTATGCAGC GGAAAGTATA CCTTAAGGAA TCCCCTGATA ACGCAGGAAA GAACATGTGA
     7801 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
     7861 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CGAGTCAGAG GTGGCGAAAC
     7921 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
     7981 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
     8041 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
     8101 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
     8161 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
     8221 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
     8281 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
     8341 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
     8401 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
     8461 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     8521 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     8581 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     8641 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     8701 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
     8761 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     8821 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     8881 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     8941 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     9001 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     9061 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     9121 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     9181 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     9241 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     9301 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     9361 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     9421 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     9481 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     9541 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
     9601 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
     9661 AGGCCCTTTC GTC
//
