LOCUS       Exported                7392 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 7, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    201..924
                     /label=CMV
     misc_feature    953..971
                     /label=T7
     misc_feature    1029..3608
                     /label=MPHOSPH8
     misc_feature    3615..3632
                     /label=6xHis Tag
     misc_feature    3670..4255
                     /label=polyA signal
     misc_feature    4531..5203
                     /label=ColE1
     misc_feature    complement(5633..6427)
                     /label=Kan/Neo
     misc_feature    complement(6462..6764)
                     /label=SV40 Ori
     misc_feature    6850..7327
                     /label=f1 ori
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcaggtt gcggagggag caagggtgac cgcagtccct gtgtcagctg
     1081 ccgacagcac tgaggagttg gccgaagtcg aagaaggagt tggagtagtg ggcgaagata
     1141 atgacgcagc cgcgagagga gcggaggcct ttggcgacag tgaggaggac ggagaggatg
     1201 tgttcgaggt ggagaagatc ctggacatga agaccgaggg gggtaaagtt ctttacaaag
     1261 ttcgctggaa aggctataca tcggatgatg atacctggga gcccgagatt cacctggagg
     1321 actgtaaaga agtgcttctt gaatttagga agaaaattgc agagaacaaa gccaaagcag
     1381 tcaggaagga tattcagaga ctatccttaa ataacgacat atttgaggcg aactctgata
     1441 gcgatcagca aagtgagaca aaagaagata cttccccaaa gaagaaaaag aaaaaattga
     1501 ggcagagaga agagaaaagc ccagatgatc tgaaaaagaa aaaagcaaag gccgggaagc
     1561 taaaagacaa gtccaaacca gacctggaga gctccttgga aagtttagtt tttgatttaa
     1621 ggacaaagaa aagaatttct gaagccaaag aagaactaaa ggagtccaaa aagcccaaaa
     1681 aagatgaagt aaaagaaaca aaagaattaa agaaagttaa aaagggtgaa ataagagatt
     1741 taaagacgaa aacaagagaa gatcccaaag aaaatagaaa aacaaaaaaa gaaaaatttg
     1801 tcgaatccca ggtggaatct gaatcaagtg tacttaatga ttctcccttt ccagaggatg
     1861 acagtgaagg gctacattcc gacagcagag aagagaaaca aaacactaaa agtgcaagag
     1921 agagagcagg gcaggacatg gggctggagc atggctttga gaagccccta gacagtgcca
     1981 tgagtgctga ggaggatacc gatgtcagag gcaggaggaa aaagaagacc ccgagaaagg
     2041 ctgaggacac tagagagaac aggaagctag agaacaagaa cgctttctta gagaagaaaa
     2101 ctgtgcctaa aaagcagagg aatcaagaca gaagcaaaag tgctgcagag ttagagaagc
     2161 tgatgcctgt atctgcccaa acgccaaagg gccggaggtt gagcggggaa gagagaggcc
     2221 tctggtccac ggactcagcc gaggaggaca aagaaaccaa aagaaatgaa tccaaagaaa
     2281 aatatcagaa aaggcatgat tctgacaagg aagaaaaagg cagaaaagag ccaaaaggat
     2341 taaagacact taaggaaatc agaaatgcat ttgatttatt taaattaact ccagaagaaa
     2401 aaaatgatgt ttctgagaat aatcggaaaa gggaagaaat accactggat tttaaaacca
     2461 tagacgatca caaaaccaag gaaaacaaac agtcacttaa agaaaggaga aacaccagag
     2521 acgaaacgga tacttgggca tacattgctg cagaaggtga tcaggaggtt ttagacagcg
     2581 tgtgccaagc agatgagaat tcagatggca ggcagcagat tctgagtttg ggcatggacc
     2641 tgcagttgga atggatgaag ttggaagatt tccaaaagca ccttgatggg aaagatgaga
     2701 attttgctgc aacagatgca attccaagta atgtgttaag ggatgctgtg aaaaatgggg
     2761 attatattac tgtaaaagtt gcacttaatt caaatgaaga atataacctg gaccaagagg
     2821 attccagtgg aatgacactg gtgatgcttg ccgccgccgg agggcaggac gacctcctgc
     2881 gactcctcat cacaaaaggc gcgaaagtga acggtcggca gaagaacggg accaccgccc
     2941 tcattcatgc tgcagagaag aactttttaa caacagtggc tattcttttg gaagcaggag
     3001 cttttgtaaa tgtccagcaa agcaatggtg agactgcact gatgaaggcc tgtaaaagag
     3061 gaaattcaga catcgtacga ctcgtaattg aatgtggagc tgactgcaat attttgtcaa
     3121 agcaccagaa tagtgccctg cactttgcga agcagtctaa caatgtgctt gtgtacgact
     3181 tgctgaagaa ccatttagag acactttcaa gagtagcaga agagacaata aaggattact
     3241 ttgaagctcg ccttgctctg ctagaaccag tttttccaat cgcatgtcat cgactctgtg
     3301 agggtccaga tttttcaaca gatttcaatt acaaaccccc acagaacata ccagaaggct
     3361 ctggcatcct gctgtttatc ttccatgcaa actttttggg taaagaagtt attgctcggc
     3421 tctgtggacc gtgtagtgta caagctgtag ttctgaatga taaatttcag cttcctgttt
     3481 ttctggacag tcattttgtt tactcattca gccctgttgc aggtcccaat aaactcttca
     3541 taaggttgac agaagcaccc tctgccaagg ttaagttgct aataggtgca tacagagtgc
     3601 agctgcagac gcgtcatcat catcaccacc actaaacggc cggccgcggt catagctgtt
     3661 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3721 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3781 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3841 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3901 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     3961 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4021 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4081 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4141 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     4201 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     4261 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     4321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4861 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5161 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     5221 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     5281 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     5341 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5401 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5461 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5521 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5581 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     5641 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     5701 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     5761 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     5821 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5881 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     5941 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     6001 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     6061 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     6121 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     6181 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     6241 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     6301 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6361 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6421 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6481 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6541 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6601 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     6661 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     6721 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     6781 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     6841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6961 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7141 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7201 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7261 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7321 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7381 aacgcgaatt tt
//
