LOCUS       Exported                7413 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 7, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    201..924
                     /label=CMV
     misc_feature    953..971
                     /label=T7
     misc_feature    1029..3608
                     /label=MPHOSPH8
     misc_feature    3609..3656
                     /label=Linker-10xHis
     misc_feature    3691..4276
                     /label=polyA signal
     misc_feature    4552..5224
                     /label=ColE1
     misc_feature    complement(5654..6448)
                     /label=Kan/Neo
     misc_feature    complement(6483..6785)
                     /label=SV40 Ori
     misc_feature    6871..7348
                     /label=f1 ori
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcaggtt gcggagggag caagggtgac cgcagtccct gtgtcagctg
     1081 ccgacagcac tgaggagttg gccgaagtcg aagaaggagt tggagtagtg ggcgaagata
     1141 atgacgcagc cgcgagagga gcggaggcct ttggcgacag tgaggaggac ggagaggatg
     1201 tgttcgaggt ggagaagatc ctggacatga agaccgaggg gggtaaagtt ctttacaaag
     1261 ttcgctggaa aggctataca tcggatgatg atacctggga gcccgagatt cacctggagg
     1321 actgtaaaga agtgcttctt gaatttagga agaaaattgc agagaacaaa gccaaagcag
     1381 tcaggaagga tattcagaga ctatccttaa ataacgacat atttgaggcg aactctgata
     1441 gcgatcagca aagtgagaca aaagaagata cttccccaaa gaagaaaaag aaaaaattga
     1501 ggcagagaga agagaaaagc ccagatgatc tgaaaaagaa aaaagcaaag gccgggaagc
     1561 taaaagacaa gtccaaacca gacctggaga gctccttgga aagtttagtt tttgatttaa
     1621 ggacaaagaa aagaatttct gaagccaaag aagaactaaa ggagtccaaa aagcccaaaa
     1681 aagatgaagt aaaagaaaca aaagaattaa agaaagttaa aaagggtgaa ataagagatt
     1741 taaagacgaa aacaagagaa gatcccaaag aaaatagaaa aacaaaaaaa gaaaaatttg
     1801 tcgaatccca ggtggaatct gaatcaagtg tacttaatga ttctcccttt ccagaggatg
     1861 acagtgaagg gctacattcc gacagcagag aagagaaaca aaacactaaa agtgcaagag
     1921 agagagcagg gcaggacatg gggctggagc atggctttga gaagccccta gacagtgcca
     1981 tgagtgctga ggaggatacc gatgtcagag gcaggaggaa aaagaagacc ccgagaaagg
     2041 ctgaggacac tagagagaac aggaagctag agaacaagaa cgctttctta gagaagaaaa
     2101 ctgtgcctaa aaagcagagg aatcaagaca gaagcaaaag tgctgcagag ttagagaagc
     2161 tgatgcctgt atctgcccaa acgccaaagg gccggaggtt gagcggggaa gagagaggcc
     2221 tctggtccac ggactcagcc gaggaggaca aagaaaccaa aagaaatgaa tccaaagaaa
     2281 aatatcagaa aaggcatgat tctgacaagg aagaaaaagg cagaaaagag ccaaaaggat
     2341 taaagacact taaggaaatc agaaatgcat ttgatttatt taaattaact ccagaagaaa
     2401 aaaatgatgt ttctgagaat aatcggaaaa gggaagaaat accactggat tttaaaacca
     2461 tagacgatca caaaaccaag gaaaacaaac agtcacttaa agaaaggaga aacaccagag
     2521 acgaaacgga tacttgggca tacattgctg cagaaggtga tcaggaggtt ttagacagcg
     2581 tgtgccaagc agatgagaat tcagatggca ggcagcagat tctgagtttg ggcatggacc
     2641 tgcagttgga atggatgaag ttggaagatt tccaaaagca ccttgatggg aaagatgaga
     2701 attttgctgc aacagatgca attccaagta atgtgttaag ggatgctgtg aaaaatgggg
     2761 attatattac tgtaaaagtt gcacttaatt caaatgaaga atataacctg gaccaagagg
     2821 attccagtgg aatgacactg gtgatgcttg ccgccgccgg agggcaggac gacctcctgc
     2881 gactcctcat cacaaaaggc gcgaaagtga acggtcggca gaagaacggg accaccgccc
     2941 tcattcatgc tgcagagaag aactttttaa caacagtggc tattcttttg gaagcaggag
     3001 cttttgtaaa tgtccagcaa agcaatggtg agactgcact gatgaaggcc tgtaaaagag
     3061 gaaattcaga catcgtacga ctcgtaattg aatgtggagc tgactgcaat attttgtcaa
     3121 agcaccagaa tagtgccctg cactttgcga agcagtctaa caatgtgctt gtgtacgact
     3181 tgctgaagaa ccatttagag acactttcaa gagtagcaga agagacaata aaggattact
     3241 ttgaagctcg ccttgctctg ctagaaccag tttttccaat cgcatgtcat cgactctgtg
     3301 agggtccaga tttttcaaca gatttcaatt acaaaccccc acagaacata ccagaaggct
     3361 ctggcatcct gctgtttatc ttccatgcaa actttttggg taaagaagtt attgctcggc
     3421 tctgtggacc gtgtagtgta caagctgtag ttctgaatga taaatttcag cttcctgttt
     3481 ttctggacag tcattttgtt tactcattca gccctgttgc aggtcccaat aaactcttca
     3541 taaggttgac agaagcaccc tctgccaagg ttaagttgct aataggtgca tacagagtgc
     3601 agctgcaggg gggtggaggc tctcaccatc accaccatca tcaccaccat cactaaacgg
     3661 ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc
     3721 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3781 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3841 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3901 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3961 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4021 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4081 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4141 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     4201 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     4261 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     4321 ggtacgcggc cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     5221 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     5281 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     5341 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     5401 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     5461 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     5521 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     5581 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     5641 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     5701 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     5761 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca
     5821 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     5881 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     5941 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     6001 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     6061 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     6121 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     6181 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     6241 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     6301 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     6361 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     6421 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     6481 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     6541 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     6601 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     6661 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6721 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     6781 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     6841 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6961 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7321 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7381 gagctgattt aacaaaaatt taacgcgaat ttt
//
