LOCUS       Exported                7221 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 7, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    201..924
                     /label=CMV
     misc_feature    953..971
                     /label=T7
     misc_feature    1029..3416
                     /label=MPHOSPH8
     misc_feature    3417..3464
                     /label=Linker-10xHis
     misc_feature    3499..4084
                     /label=polyA signal
     misc_feature    4360..5032
                     /label=ColE1
     misc_feature    complement(5462..6256)
                     /label=Kan/Neo
     misc_feature    complement(6291..6593)
                     /label=SV40 Ori
     misc_feature    6679..7156
                     /label=f1 ori
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcaggtt gcggagggag caagggtgac cgcagtccct gtgtcagctg
     1081 ccgacagcac tgaggagttg gccgaagtcg aagaaggagt tggagtagtg ggcgaagata
     1141 atgacgcagc cgcgagagga gcggaggcct ttggcgacag tgaggaggac aggaaggata
     1201 ttcagagact atccttaaat aacgacatat ttgaggcgaa ctctgatagc gatcagcaaa
     1261 gtgagacaaa agaagatact tccccaaaga agaaaaagaa aaaattgagg cagagagaag
     1321 agaaaagccc agatgatctg aaaaagaaaa aagcaaaggc cgggaagcta aaagacaagt
     1381 ccaaaccaga cctggagagc tccttggaaa gtttagtttt tgatttaagg acaaagaaaa
     1441 gaatttctga agccaaagaa gaactaaagg agtccaaaaa gcccaaaaaa gatgaagtaa
     1501 aagaaacaaa agaattaaag aaagttaaaa agggtgaaat aagagattta aagacgaaaa
     1561 caagagaaga tcccaaagaa aatagaaaaa caaaaaaaga aaaatttgtc gaatcccagg
     1621 tggaatctga atcaagtgta cttaatgatt ctccctttcc agaggatgac agtgaagggc
     1681 tacattccga cagcagagaa gagaaacaaa acactaaaag tgcaagagag agagcagggc
     1741 aggacatggg gctggagcat ggctttgaga agcccctaga cagtgccatg agtgctgagg
     1801 aggataccga tgtcagaggc aggaggaaaa agaagacccc gagaaaggct gaggacacta
     1861 gagagaacag gaagctagag aacaagaacg ctttcttaga gaagaaaact gtgcctaaaa
     1921 agcagaggaa tcaagacaga agcaaaagtg ctgcagagtt agagaagctg atgcctgtat
     1981 ctgcccaaac gccaaagggc cggaggttga gcggggaaga gagaggcctc tggtccacgg
     2041 actcagccga ggaggacaaa gaaaccaaaa gaaatgaatc caaagaaaaa tatcagaaaa
     2101 ggcatgattc tgacaaggaa gaaaaaggca gaaaagagcc aaaaggatta aagacactta
     2161 aggaaatcag aaatgcattt gatttattta aattaactcc agaagaaaaa aatgatgttt
     2221 ctgagaataa tcggaaaagg gaagaaatac cactggattt taaaaccata gacgatcaca
     2281 aaaccaagga aaacaaacag tcacttaaag aaaggagaaa caccagagac gaaacggata
     2341 cttgggcata cattgctgca gaaggtgatc aggaggtttt agacagcgtg tgccaagcag
     2401 atgagaattc agatggcagg cagcagattc tgagtttggg catggacctg cagttggaat
     2461 ggatgaagtt ggaagatttc caaaagcacc ttgatgggaa agatgagaat tttgctgcaa
     2521 cagatgcaat tccaagtaat gtgttaaggg atgctgtgaa aaatggggat tatattactg
     2581 taaaagttgc acttaattca aatgaagaat ataacctgga ccaagaggat tccagtggaa
     2641 tgacactggt gatgcttgcc gccgccggag ggcaggacga cctcctgcga ctcctcatca
     2701 caaaaggcgc gaaagtgaac ggtcggcaga agaacgggac caccgccctc attcatgctg
     2761 cagagaagaa ctttttaaca acagtggcta ttcttttgga agcaggagct tttgtaaatg
     2821 tccagcaaag caatggtgag actgcactga tgaaggcctg taaaagagga aattcagaca
     2881 tcgtacgact cgtaattgaa tgtggagctg actgcaatat tttgtcaaag caccagaata
     2941 gtgccctgca ctttgcgaag cagtctaaca atgtgcttgt gtacgacttg ctgaagaacc
     3001 atttagagac actttcaaga gtagcagaag agacaataaa ggattacttt gaagctcgcc
     3061 ttgctctgct agaaccagtt tttccaatcg catgtcatcg actctgtgag ggtccagatt
     3121 tttcaacaga tttcaattac aaacccccac agaacatacc agaaggctct ggcatcctgc
     3181 tgtttatctt ccatgcaaac tttttgggta aagaagttat tgctcggctc tgtggaccgt
     3241 gtagtgtaca agctgtagtt ctgaatgata aatttcagct tcctgttttt ctggacagtc
     3301 attttgttta ctcattcagc cctgttgcag gtcccaataa actcttcata aggttgacag
     3361 aagcaccctc tgccaaggtt aagttgctaa taggtgcata cagagtgcag ctgcaggggg
     3421 gtggaggctc tcaccatcac caccatcatc accaccatca ctaaacggcc ggccgcggtc
     3481 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     3541 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     3601 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     3661 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     3721 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     3781 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     3841 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3901 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3961 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     4021 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     4081 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     4141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4681 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     5041 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     5101 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     5161 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     5221 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     5281 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     5341 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     5401 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     5461 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     5521 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     5581 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     5641 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     5701 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     5761 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     5821 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     5881 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     5941 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     6001 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     6061 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     6121 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     6181 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     6241 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     6301 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6361 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6421 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6481 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6541 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6601 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     6661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6781 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6961 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7021 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7081 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7141 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7201 caaaaattta acgcgaattt t
//
