LOCUS       Exported                6156 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 7, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    201..924
                     /label=CMV
     misc_feature    953..971
                     /label=T7
     misc_feature    1029..2351
                     /label=MPHOSPH8
     misc_feature    2352..2399
                     /label=Linker-10xHis
     misc_feature    2434..3019
                     /label=polyA signal
     misc_feature    3295..3967
                     /label=ColE1
     misc_feature    complement(4397..5191)
                     /label=Kan/Neo
     misc_feature    complement(5226..5528)
                     /label=SV40 Ori
     misc_feature    5614..6091
                     /label=f1 ori
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcaggtt gcggagggag caagggtgac cgcagtccct gtgtcagctg
     1081 ccgacagcac tgaggagttg gccgaagtcg aagaaggagt tggagtagtg ggcgaagata
     1141 atgacgcagc cgcgagagga gcggaggcct ttggcgacag tgaggaggac ggagaggatg
     1201 tgttcgaggt ggagaagatc ctggacatga agaccgaggg gggtaaagtt ctttacaaag
     1261 ttcgctggaa aggctataca tcggatgatg atacctggga gcccgagatt cacctggagg
     1321 actgtaaaga agtgcttctt gaatttagga agaaaattgc agagaacaaa gccaaagcag
     1381 tcaggaagga tattcagaga ctatccttaa ataacgacat atttgaggcg aactctgata
     1441 gcgatcagca aagtgagaca aaagaagata cttccccaaa gaagaaaaag aaaaaattga
     1501 ggcagagaga agagaaaagc ccagatgatc tgaaaaagaa aaaagcaaag gccgggaagc
     1561 taaaagacaa gtccaaacca gacctggaga gctccttgga aagtttagtt tttgatttaa
     1621 ggacaaagaa aagaatttct gaagccaaag aagaactaaa ggagtccaaa aagcccaaaa
     1681 aagatgaagt aaaagaaaca aaagaattaa agaaagttaa aaagggtgaa ataagagatt
     1741 taaagacgaa aacaagagaa gatcccaaag aaaatagaaa aacaaaaaaa gaaaaatttg
     1801 tcgaatccca ggtggaatct gaatcaagtg tacttaatga ttctcccttt ccagaggatg
     1861 acagtgaagg gctacattcc gacagcagag aagagaaaca aaacactaaa agtgcaagag
     1921 agagagcagg gcaggacatg gggctggagc atggctttga gaagccccta gacagtgcca
     1981 tgagtgctga ggaggatacc gatgtcagag gcaggaggaa aaagaagacc ccgagaaagg
     2041 ctgaggacac tagagagaac aggaagctag agaacaagaa cgctttctta gagaagaaaa
     2101 ctgtgcctaa aaagcagagg aatcaagaca gaagcaaaag tgctgcagag ttagagaagc
     2161 tgatgcctgt atctgcccaa acgccaaagg gccggaggtt gagcggggaa gagagaggcc
     2221 tctggtccac ggactcagcc gaggaggaca aagaaaccaa aagaaatgaa tccaaagaaa
     2281 aatatcagaa aaggcatgat tctgacaagg aagaaaaagg cagaaaagag ccaaaaggat
     2341 taaagacact tgggggtgga ggctctcacc atcaccacca tcatcaccac catcactaaa
     2401 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     2461 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2521 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2581 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2641 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     2701 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     2761 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     2821 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     2881 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     2941 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     3001 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     3061 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     3961 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     4021 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     4081 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     4141 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4201 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4261 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4321 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4381 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4441 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4501 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4561 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4621 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4681 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4741 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4801 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4861 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4921 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4981 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5041 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5101 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5161 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5221 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5281 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5341 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5401 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5461 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5521 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5581 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5701 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5761 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5821 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5881 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5941 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6001 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6061 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6121 aatgagctga tttaacaaaa atttaacgcg aatttt
//
