LOCUS       Exported                7026 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 7, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    201..924
                     /label=CMV
     misc_feature    953..971
                     /label=T7
     misc_feature    1029..3221
                     /label=MPHOSPH8
     misc_feature    3222..3269
                     /label=Linker-10xHis
     misc_feature    3304..3889
                     /label=polyA signal
     misc_feature    4165..4837
                     /label=ColE1
     misc_feature    complement(5267..6061)
                     /label=Kan/Neo
     misc_feature    complement(6096..6398)
                     /label=SV40 Ori
     misc_feature    6484..6961
                     /label=f1 ori
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcaggtt gcggagggag caagggtgac cgcagtccct gtgtcagctg
     1081 ccgacagcac tgaggagttg gccgaagtcg aagaaggagt tggagtagtg ggcgaagata
     1141 atgacgcagc cgcgagagga gcggaggcct ttggcgacag tgaggaggac ggagaggatg
     1201 tgttcgaggt ggagaagatc ctggacatga agaccgaggg gggtaaagtt ctttacaaag
     1261 ttcgctggaa aggctataca tcggatgatg atacctggga gcccgagatt cacctggagg
     1321 actgtaaaga agtgcttctt gaatttagga agaaaattgc agagaacaaa gccaaagcag
     1381 tcaggaagga tattcagaga ctatccttaa ataacgacat atttgaggcg aactctgata
     1441 gcgatcagca aagtgagaca aaagaagata cttccccaaa gaagaaaaag aaaaaattga
     1501 ggcagagaga agagaaaagc ccagatgatc tgaaaaagaa aaaagcaaag gccgggaagc
     1561 taaaagacaa gtccaaacca gacctggaga gctccttgga aagtttagtt tttgatttaa
     1621 ggacaaagaa aagaatttct gaagccaaag aagaactaaa ggagtccaaa aagcccaaaa
     1681 aagatgaagt aaaagaaaca aaagaattaa agaaagttaa aaagggtgaa ataagagatt
     1741 taaagacgaa aacaagagaa gatcccaaag aaaatagaaa aacaaaaaaa gaaaaatttg
     1801 tcgaatccca ggtggaatct gaatcaagtg tacttaatga ttctcccttt ccagaggatg
     1861 acagtgaagg gctacattcc gacagcagag aagagaaaca aaacactaaa agtgcaagag
     1921 agagagcagg gcaggacatg gggctggagc atggctttga gaagccccta gacagtgcca
     1981 tgagtgctga ggaggatacc gatgtcagag gcaggaggaa aaagaagacc ccgagaaagg
     2041 ctgaggacac tagagagaac aggaagctag agaacaagaa cgctttctta gagaagaaaa
     2101 ctgtgcctaa aaagcagagg aatcaagaca gaagcaaaag tgctgcagag ttagagaagc
     2161 tgatgcctgt atctgcccaa acgccaaagg gccggaggtt gagcggggaa gagagaggcc
     2221 tctggtccac ggactcagcc gaggaggaca aagaaaccaa aagaaatgaa tccaaagaaa
     2281 aatatcagaa aaggcatgat tctgacaagg aagaaaaagg cagaaaagag ccaaaaggat
     2341 taaagacact taaggaaatc agaaatgcat ttgatttatt taaattaact ccagaagaaa
     2401 aaaatgatgt ttctgagaat aatcggaaaa gggaagaaat accactggat tttaaaacca
     2461 tagacgatca caaaaccaag gaaaacaaac agtcacttaa agaaaggaga aacaccagag
     2521 acgaaacgga tacttgggca tacattgctg cagaaggtga tcaggaggtt ttagacagcg
     2581 tgtgccaagc agatgagaat tcagatggca ggcagcagat tctgagtttg ggcatggacc
     2641 tgcagttgga atggatgaag ttggaagatt tccaaaagca ccttgatggg aaagatgaga
     2701 attttgctgc aacagatgca attccaagta atgtgttaag ggatgctgtg aaaaatgggg
     2761 attatattac tgtaaaagtt gcacttaatt caaatgaaga atataacctg gaccaagagg
     2821 attccgtagc agaagagaca ataaaggatt actttgaagc tcgccttgct ctgctagaac
     2881 cagtttttcc aatcgcatgt catcgactct gtgagggtcc agatttttca acagatttca
     2941 attacaaacc cccacagaac ataccagaag gctctggcat cctgctgttt atcttccatg
     3001 caaacttttt gggtaaagaa gttattgctc ggctctgtgg accgtgtagt gtacaagctg
     3061 tagttctgaa tgataaattt cagcttcctg tttttctgga cagtcatttt gtttactcat
     3121 tcagccctgt tgcaggtccc aataaactct tcataaggtt gacagaagca ccctctgcca
     3181 aggttaagtt gctaataggt gcatacagag tgcagctgca ggggggtgga ggctctcacc
     3241 atcaccacca tcatcaccac catcactaaa cggccggccg cggtcatagc tgtttcctga
     3301 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     3361 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     3421 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     3481 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     3541 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     3601 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     3661 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     3721 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     3781 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     3841 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     3901 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     3961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4801 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     4861 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     4921 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     4981 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5041 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5101 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5161 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5221 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     5281 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5341 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5401 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     5461 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     5521 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     5581 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     5641 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     5701 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     5761 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     5821 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     5881 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     5941 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6001 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6061 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6121 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     6181 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6241 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6301 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6361 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6421 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     6481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     6601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6781 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6901 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6961 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7021 aatttt
//
