LOCUS       Addgene_CJ8421_04975_Rp 3488 bp ds-DNA     circular     13-MAR-2023
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer          158..179
                     /label="CJpr0298 pCSM180 backbone sequencing primer F"
                     /note="sequence: tcgggtttcgccacctctgact"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    316..333
                     /label="mark"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    363..417
                     /label="OR2-OR1-Pr"
                     /ApEinfo_revcolor="#ff2460"
                     /ApEinfo_fwdcolor="#ff2460"
     misc_feature    368..384
                     /label="OR2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    392..408
                     /label="OR1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    398..585
                     /label="CJfr0290 Sth1Cas9 locus 2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(418..455)
                     /label="Sth1Cas9 locus2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          456..477
                     /label="CJpr0568 Sth1Cas9 nexus F"
                     /note="sequence: AAGGCTTCATGCCGAAATCAAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    456..505
                     /label="Nexus and hairpins of Sth1Cas9 tracrRNA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    506..535
                     /label="T500 terminator"
                     /ApEinfo_revcolor="#ff1115"
                     /ApEinfo_fwdcolor="#ff1115"
     misc_feature    536..565
                     /label="30bp random sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    566..600
                     /label="J23119 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          581..619
                     /label="CJpr0438 J23119 promoter -peak113-F"
                     /note="sequence: GTCCTAGGTATAATGCTAGCaatttccttccaagctcca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          601..622
                     /label="CJpr0472 peak113-F"
                     /note="sequence: aatttccttccaagctccatct"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     gene            complement(601..822)
                     /label="CJ8421_04980"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             921..1439
                     /label="CJ8421_04975"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1039..1058
                     /label="Locus2 spacer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1059..1094
                     /label="Sth1Cas9 locus2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            1436..1739
                     /label="CJ8421_04970"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            1740..1789
                     /label="CJ8421_04970"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1766..1789)
                     /label="CJpr0473 peak113-R"
                     /note="sequence: ctctccaagctccattaaactagg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1770..1810)
                     /label="CJpr0451 T7 teminator-peak113- New-F"
                     /note="sequence: CCTTTCGGGCTTTGTTAGCAGctctccaagctccattaaac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1790..1810
                     /label="CJpr0303 T7 terminator F"
                     /note="sequence: CTGCTAACAAAGCCCGAAAGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1790..1918
                     /label="T7 teminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1895..1942)
                     /label="CJpr0291 T7 terninator- Amp-R"
                     /note="sequence: GGATCGATCGATTCTTTCaagcttATCCGGATATAGTTCCTCCTTTCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1919..1944
                     /label="CJpr0297 Amp upstream F"
                     /note="sequence: aagcttGAAAGAATCGATCGATCCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      complement(1966..2062)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2063..2920)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     primer          complement(2077..2097)
                     /label="CJpr0299 pCSM180 backbone sequencing primer R"
                     /note="sequence: tcgctgagataggtgcctcac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(2921..3051)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(3052..327)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
       61 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      301 ttatcccctg attctgtgga taaccgtgcg gccCGAGAGC ATCGATCCTA GCATGGatgc
      361 atTGAGCTAA CACCGTGCGT GTTGACAATT TTACCTCTGG CGGTGATAAT GGTTGCAaac
      421 tatcatattt aatataagat gagAGCtaaa tttcTAAGGC TTCATGCCGA AATCAACACC
      481 CTGTCATTTT ATGGCAGGGT GTTTTCAAAG CCCGCCGAAA GGCGGGCTTT TCTGTAATAC
      541 CTCGACACTG GGATACTGTA GGATCTTGAC AGCTAGCTCA GTCCTAGGTA TAATGCTAGC
      601 aatttccttc caagctccat ctatatcaaa ttcaagctct gttccatcgc taagatagac
      661 ttcataggaa tttttgtctt tttgcacgat ccctataggc gctttgaaat tatgttgtaa
      721 aaattcttta gaaacatttg gtaaattatc agcagaaatt attatatcag catttaaaga
      781 taaacttaaa atagtaataa tatttaaaaa tttaattttc aatatttttc ctaaattatt
      841 ttttagtaaa attgtaaaga atttatgtga attttatgtg aattttaaga caatattata
      901 aaaaataaag gttttatatt ttgcttacaa tttttttaat ttttttaaat attttatgtt
      961 attttttgat ttcttatgat tattacaata tccttggttt gaatatttta ttttttaatg
     1021 gagcttattg gcagctttta agttcaatgt ttttacatgg aaatttaact catcttatat
     1081 taaatatgat agttttgttt caatttgggc gtatgttaga aacttatttg ggtgctttgt
     1141 gttttatttt tatttatttt attggcggat tgttgtgctc acttttaagt gttttttatg
     1201 tgtattttga ttttaaatat tttggagaaa atataaatgt cataggcgct agtggtgcaa
     1261 tttgtgtttt aatgggcttt tatgctgtta ttgataaaaa tagcacaaaa ggacttatag
     1321 tggcaatttt acttatgagt tttgtgccat tgcttatggg gataaatgtt gcttggtatg
     1381 gtcatatttt cgggtttatg tgtggttata ttttagctaa aataaaagag gtaaaatgaa
     1441 aaaaatttat attataagac atgcaaaagc aagtaagagt gaagacatcg atgattttga
     1501 aagaaagctt actaagagtg gaaaggaaga tttaaaaaaa ctttttaaaa atcttgcttc
     1561 gcatgaaatt catcctgatt tggtgctatc aagtcctgct gttagaaccg ctaaaacagc
     1621 gaaaaaaata gccaaatttt ataattttga taaaaataga atttgttttg atgaaagatt
     1681 gtatctttgc aatgttgaaa atttgctaaa aattctgcaa gatattgatg atgagtttaa
     1741 tgaagtattt ttagtgggtc ataatcctag tttaatggag cttggagagC TGCTAACAAA
     1801 GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT
     1861 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATaa
     1921 gcttGAAAGA ATCGATCGAT CCCGAATGAG TAGtacagcg gccgcgatta tcaaaaagga
     1981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2161 agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc
     2221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca
     3061 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3481 gaacgacc
//