LOCUS       Exported                8892 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pAN2414.gb
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    684..1447
                     /label=ColE1
                     /note="color: #7f7f7f"
     misc_feature    1448..1578
                     /label=AmpR Promoter
                     /note="color: #f58a5e"
     misc_feature    1579..2436
                     /label=AmpR
                     /note="color: #9f4240"
     misc_feature    2437..2533
                     /label=AmpR Terminator
                     /note="color: #f58a5e"
     misc_feature    complement(2437..2533)
                     /label=AmpR Terminator
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    4452..4457
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    4460..4463
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    5203..5208
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    5211..5214
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    complement(7378..7379)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7379..7389)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7390..7393)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7395..7400)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    7401..7566
                     /label=E. coli GPDH Promoter
                     /note="color: #b1ff67"
     misc_feature    7401..7530
                     /label=pglpT-jtk2821
                     /note="color: #2bb232"
     misc_feature    7401..7530
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #b1ff67"
     misc_feature    7531..7566
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #b1ff67"
     misc_feature    7549..7560
                     /label=Elowitz RBS (BBa_B0034)
                     /note="color: #75c6a9"
     misc_feature    7567..8283
                     /label=sfGFP
                     /note="color: #b1ff67"
     misc_feature    7570..8280
                     /label=sfGFP
                     /note="color: #c7b0e3"
     misc_feature    8284..8412
                     /label=BBa_B0015 Terminator
                     /note="color: #b1ff67"
     misc_feature    8284..8363
                     /label=Ec_rrfB + T7_TE (BBa_B0015)
                     /note="color: #d59687"
     misc_feature    8284..8363
                     /label=Ec_rrfBt1 (BBa_B0010, TrrnB1)
                     /note="color: #d59687"
     misc_feature    8372..8412
                     /label=Ec_rrfB + T7_TE (BBa_B0015)
                     /note="color: #d59687"
     misc_feature    8372..8412
                     /label=T7_TE (BBa_B0012) T71.3t
                     /note="color: #d59687"
     misc_feature    8413..8418
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    8420..8423
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    8424..8429
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    8431..8434
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    8523..8528
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    8531..8534
                     /label=Bbs1
                     /note="color: #b1ff67"
ORIGIN
        1 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
       61 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
      121 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      181 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      241 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      301 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      361 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg
      421 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      481 cttgtctgct cccggcatcc gcttacagac aagctgtgac catctccggg agctgcatgt
      541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagatg aaagggcctc gtgatacgcc
      601 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      661 ggggaaatgt gccgagcggc cgcacggtta tccacagaat caggggataa cgcaggaaag
      721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1441 gtcatgacag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1501 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1561 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1621 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1681 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1741 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1801 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1861 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1921 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1981 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2041 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2101 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2281 gccggtgagc gtgggtcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2401 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2461 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2521 ctttttgata atcgcggccg ccaatttttt acggttcctg gccttttgct ggccttttgc
     2581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2641 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2701 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2761 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2821 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3001 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3061 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
     3121 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     3181 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     3241 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     3301 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     3361 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     3421 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
     3481 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
     3541 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
     3601 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
     3661 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
     3721 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
     3781 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
     3841 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
     3901 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
     3961 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     4021 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
     4081 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
     4141 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
     4201 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     4261 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     4321 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     4381 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     4441 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     4501 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     4561 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     4621 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     4681 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     4741 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     4801 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4861 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4981 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     5041 cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact
     5101 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     5161 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     5221 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     5281 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     5341 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     5401 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     5461 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     5521 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     5581 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggactctc
     5641 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5701 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5761 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     5821 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     5881 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     5941 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6001 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6061 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6121 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6181 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6241 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6301 gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata tatatgaggg
     6361 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     6421 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     6481 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6541 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     6601 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     6661 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     6721 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     6781 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     6841 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     6901 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     6961 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7021 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7081 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     7141 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtcgccaa
     7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggccctct
     7381 gatgagacga acgtgagacc gaaagtgaaa cgtgatttca tgcgtcattt tgaacatttt
     7441 gtaaatctta tttaataatg tgtgcggcaa ttcacattta atttatgaat gttttcttaa
     7501 catcgcggca actcaagaaa cggcaggttc ggatcttagc tactagagaa agaggagaaa
     7561 tactagatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
     7621 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
     7681 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt tccttggccg
     7741 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
     7801 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
     7861 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
     7921 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aggacggcaa tatcctgggc
     7981 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
     8041 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
     8101 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
     8161 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
     8221 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
     8281 tgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     8341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     8401 tctgcgttta taggtctcag ctgcgtctca agcatacacg ataccgtcga ccaaggcagc
     8461 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8521 acgaagacaa gatctgcttt ttgcttgtac tgggtttctc tggttagacc agatctgagc
     8581 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8641 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8701 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     8761 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     8821 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     8881 tcatctctct cg
//
