LOCUS       Exported                8844 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pAN2424.gb
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..11)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(12..15)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(17..22)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    23..188
                     /label=E. coli GPDH Promoter
                     /note="color: #b1ff67"
     misc_feature    23..152
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #b1ff67"
     misc_feature    23..152
                     /label=pglpT-jtk2821
                     /note="color: #2bb232"
     misc_feature    153..188
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #b1ff67"
     misc_feature    171..182
                     /label=Elowitz RBS (BBa_B0034)
                     /note="color: #75c6a9"
     misc_feature    189..905
                     /label=sfGFP
                     /note="color: #b1ff67"
     primer_bind     complement(189..206)
                     /label=MTK_P274
                     /note="color: black; sequence: ctcttcgcctttacgcat"
     misc_feature    192..902
                     /label=sfGFP
                     /note="color: #75c6a9"
     misc_feature    257..273
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e"
     misc_feature    complement(257..273)
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e; direction: LEFT"
     terminator      906..1034
                     /label=BBa_B0015 Terminator
                     /note="color: #b1ff67"
     misc_feature    906..985
                     /label=Ec_rrfBt1 (BBa_B0010, TrrnB1)
                     /note="color: #d59687"
     misc_feature    906..985
                     /label=Ec_rrfB + T7_TE (BBa_B0015)
                     /note="color: #d59687"
     misc_feature    994..1034
                     /label=T7_TE (BBa_B0012) T71.3t
                     /note="color: #d59687"
     misc_feature    994..1034
                     /label=Ec_rrfB + T7_TE (BBa_B0015)
                     /note="color: #d59687"
     misc_feature    1035..1040
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    1042..1045
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    1046..1051
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    1053..1056
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    1192..1197
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    1200..1203
                     /label=Bbs1
                     /note="color: #b1ff67"
     promoter        complement(1426..1444)
                     /label=SP6 promoter
                     /note="color: #85dae9; direction: LEFT"
     primer_bind     complement(1427..1444)
                     /label=SP6
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1684..1700
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e"
     misc_feature    complement(1684..1700)
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    complement(1874..1881)
                     /label=Kozak Sequence
                     /note="color: #d59687; direction: LEFT"
     misc_feature    2245..3008
                     /label=ColE1
                     /note="color: #7f7f7f"
     rep_origin      complement(2326..3008)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     misc_feature    complement(2326..3008)
                     /label=Col E1 Origin
                     /note="color: #b1ff67; direction: LEFT"
     primer_bind     complement(2339..2356)
                     /label=MTK_P072
                     /note="color: black; sequence: gagcctatggaaaaacgc"
     misc_feature    2854..2870
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e"
     misc_feature    complement(2854..2870)
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    3009..3156
                     /label=KanR Promoter
                     /note="color: #b1ff67"
     misc_feature    3157..3972
                     /label=KanR
                     /note="color: #008000"
     misc_feature    3973..3999
                     /label=KanR Terminator
                     /note="color: #b1ff67"
     misc_feature    4012..8840
                     /label=LentiBackboneDS
                     /note="color: #d6b295"
     promoter        4252..4568
                     /label=SV40 promoter
                     /note="color: #84b0dc"
     promoter        4372..4568
                     /label=SV40 early promoter
                     /note="color: #84b0dc"
     misc_feature    5918..5923
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    5926..5929
                     /label=Bbs1
                     /note="color: #b1ff67"
     polyA_signal    complement(6281..6472)
                     /label=SV40 late polyA
                     /note="color: #d59687; direction: LEFT"
     misc_feature    6669..6674
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    6677..6680
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    7325..7450
                     /label=HIV-1 Psi
                     /note="color: #f8d3a9"
     misc_feature    7942..8175
                     /label=RRE
                     /note="color: #f8d3a9"
     misc_feature    8661..8840
                     /label=cPPT
                     /note="color: #dc143c"
     misc_feature    complement(join(8844,1))
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
ORIGIN
        1 ctgatgagac gaacgtgaga ccgaaagtga aacgtgattt catgcgtcat tttgaacatt
       61 ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga atgttttctt
      121 aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag aaagaggaga
      181 aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      241 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      301 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gttccttggc
      361 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
      421 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      481 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      541 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc aatatcctgg
      601 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
      661 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
      721 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
      781 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
      841 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
      901 aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      961 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1021 tttctgcgtt tataggtctc agctgcgtct caagcataca cgataccgtc gacctcgagg
     1081 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     1141 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     1201 atctgctttt tgcttgtact gggtttctct ggttagacca gatctgagcc tgggagctct
     1261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     1321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     1381 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     1441 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     1501 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     1561 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     1621 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     1681 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     1741 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     1801 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     1861 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     1921 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctagctct
     1981 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2041 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccatctccgg gagctgcatg
     2101 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagat gaaagggcct cgtgatacgc
     2161 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2221 cggggaaatg tgccgagcgg ccgcacggtt atccacagaa tcaggggata acgcaggaaa
     2281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2761 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3001 ggtcatgact gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg
     3061 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     3121 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     3181 tcttgctcca ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     3241 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     3301 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     3361 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     3421 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
     3481 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     3541 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc
     3601 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3661 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc
     3721 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3781 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     3841 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     3901 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     3961 gagtttttct aatcagaatt ggttaattgg ttgtaacacg cggccgccaa ttttttacgg
     4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4141 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4201 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     4261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4321 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4381 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4501 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4561 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     4621 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     4681 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     4741 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     4801 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     4861 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     4921 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     4981 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     5041 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     5101 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     5161 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     5221 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     5281 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     5341 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     5401 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     5461 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     5521 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     5581 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     5641 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     5701 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     5761 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     5821 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     5881 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     5941 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     6001 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     6061 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     6121 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     6181 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     6241 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6301 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     6481 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     6541 ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     6601 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     6661 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6721 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     6781 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     6841 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     6901 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     6961 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     7021 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     7081 tgctttttgc ctgtactggg actctctggt tagaccagat ctgagcctgg gagctctctg
     7141 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7201 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7261 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7321 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7381 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7441 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7501 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7561 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7621 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7681 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7741 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggtgatct
     7801 tcagacctgg acgatatata tgagggacaa ttggagaagt gaattatata aatataaagt
     7861 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7921 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7981 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8041 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8101 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8161 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8221 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8281 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8341 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8401 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8461 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8521 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     8581 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     8641 agtgaacgga tctcgacggt cgccaaatgg cagtattcat ccacaatttt aaaagaaaag
     8701 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8761 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     8821 acagcagaga tccagtttgg ccct
//
