LOCUS       Exported                3279 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pAN1031.gb
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    complement(57..820)
                     /label=ColE1
                     /note="color: #7f7f7f; direction: LEFT"
     misc_feature    57..739
                     /label=Col E1 Origin
                     /note="color: #b1ff67"
     misc_feature    195..211
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e"
     misc_feature    complement(195..211)
                     /label=Gal4p-Binding Site
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    825..830
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    832..835
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    836..2446
                     /label=Notch-Gal4VP64
                     /note="color: #75c6a9"
     misc_feature    836..1810
                     /label=NotchCore
                     /note="color: #f8d3a9"
     CDS             836..1810
                     /codon_start=1
                     /label=Translation 836-1810
                     /note="color: #993366"
                     /translation="ILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHA
                     CGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNP
                     LYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNS
                     FHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTS
                     GGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYK
                     IEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRR"
     misc_feature    1733..1795
                     /label=Notch TMD
                     /note="color: #84b0dc"
     misc_feature    1787..2446
                     /label=NLS-Gal4VP64
                     /note="color: #b4abac"
     misc_feature    1787..1813
                     /label=NLS
                     /note="color: #8882be"
     CDS             1811..1813
                     /codon_start=1
                     /label=Translation 1811-1813
                     /note="color: #993366"
                     /translation="R"
     CDS             1814..2290
                     /codon_start=1
                     /label=Translation 1814-2290
                     /note="color: #993366"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSAAAGGSGGSGGS"
     misc_feature    2291..2440
                     /label=VP64
                     /note="color: #d59687"
     misc_feature    complement(2449..2452)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(2454..2459)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(2462..2570)
                     /label=CamR Terminator
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    complement(2571..3230)
                     /label=CamR
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(join(3231..3279,1..56))
                     /label=CamR Promoter
                     /note="color: #84b0dc; direction: LEFT"
ORIGIN
        1 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
       61 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      781 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc attctatcct
      841 ggactacagc ttcacaggtg gcgctgggcg cgacattccc ccaccgcaga ttgaggaggc
      901 ctgtgagctg cctgagtgcc aggtggatgc aggcaataag gtctgcaacc tgcagtgtaa
      961 taatcacgca tgtggctggg atggtggcga ctgctccctc aacttcaatg acccctggaa
     1021 gaactgcacg cagtctctac agtgctggaa gtattttagc gacggccact gtgacagcca
     1081 gtgcaactcg gccggctgcc tctttgatgg cttcgactgc cagctcaccg agggacagtg
     1141 caaccccctg tatgaccagt actgcaagga ccacttcagt gatggccact gcgaccaggg
     1201 ctgtaacagt gccgaatgtg agtgggatgg cctagactgt gctgagcatg tacccgagcg
     1261 gctggcagcc ggcaccctgg tgctggtggt gctgcttcca cccgaccagc tacggaacaa
     1321 ctccttccac tttctgcggg agctcagcca cgtgctgcac accaacgtgg tcttcaagcg
     1381 tgatgcgcaa ggccagcaga tgatcttccc gtactatggc cacgaggaag agctgcgcaa
     1441 gcacccaatc aagcgctcta cagtgggttg ggccacctct tcactgcttc ctggtaccag
     1501 tggtgggcgc cagcgcaggg agctggaccc catggacatc cgtggctcca ttgtctacct
     1561 ggagatcgac aaccggcaat gtgtgcagtc atcctcgcag tgcttccaga gtgccaccga
     1621 tgtggctgcc ttcctaggtg ctcttgcgtc acttggcagc ctcaatattc cttacaagat
     1681 tgaggccgtg aagagtgagc cggtggagcc tccgctgccc tcgcagctgc acctcatgta
     1741 cgtggcagcg gctgccttcg tgctcctgtt ctttgtgggc tgtggggtgc tgctgtcccg
     1801 caagcgccgg cggatgaagc tgctgagcag catcgagcag gcctgtgaca tctgccggct
     1861 gaagaaactg aagtgcagca aagaaaagcc caagtgcgcc aagtgcctga agaacaactg
     1921 ggagtgccgg tacagcccca agaccaagag aagccccctg accagagccc acctgaccga
     1981 ggtggaaagc cggctggaaa gactggaaca gctgtttctg ctgatcttcc cacgcgagga
     2041 cctggacatg atcctgaaga tggacagcct gcaggacatc aaggccctgc tgaccggcct
     2101 gttcgtgcag gacaacgtga acaaggacgc cgtgaccgac agactggcca gcgtggaaac
     2161 cgacatgccc ctgaccctgc ggcagcacag aatcagcgcc accagcagca gcgaggaaag
     2221 cagcaacaag ggccagcggc agctgacagt gtctgctgct gcaggcggaa gcggaggctc
     2281 tggcggatct gatgccctgg acgacttcga cctggatatg ctgggcagcg acgccctgga
     2341 tgattttgat ctggacatgc tgggatctga cgctctggac gatttcgatc tcgacatgtt
     2401 gggatcagat gcactggatg actttgacct ggacatgctc ggatcaggat cctgagacca
     2461 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2521 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     2581 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     2641 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2701 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2761 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     2821 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2881 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2941 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     3001 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     3061 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     3121 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     3181 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     3241 ttagctcctg aaaatctcga taactcaaaa aatacgccc
//
