LOCUS       Exported               10921 bp ds-DNA     circular SYN 07-NOV-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..6
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    1..6
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    8..11
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    8..11
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    100..105
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    108..111
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    1153..1916
                     /label=ColE1
                     /note="color: #7f7f7f"
     misc_feature    1153..1916
                     /label=ColE1
                     /note="color: #7f7f7f"
     misc_feature    1917..2047
                     /label=AmpR Promoter
                     /note="color: #f58a5e"
     misc_feature    1917..2047
                     /label=AmpR Promoter
                     /note="color: #f58a5e"
     misc_feature    2048..2905
                     /label=AmpR
                     /note="color: #9f4240"
     misc_feature    2048..2905
                     /label=AmpR
                     /note="color: #9f4240"
     misc_feature    2906..3002
                     /label=AmpR Terminator
                     /note="color: #f58a5e"
     misc_feature    complement(2906..3002)
                     /label=AmpR Terminator
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    4921..4926
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    4929..4932
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    5672..5677
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    5680..5683
                     /label=Bbs1
                     /note="color: #b1ff67"
     misc_feature    complement(7847..7848)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7847..7848)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7848..7858)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(7848..7858)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    7863..9052
                     /label=pEF1a(TTK)
                     /note="color: #84b0dc"
     misc_feature    9056..9115
                     /label=Signal Sequence
                     /note="color: #85dae9"
     misc_feature    9116..9145
                     /label=Myc
                     /note="color: #9eafd2"
     misc_feature    9146..9175
                     /label=2xGGGGS linker
                     /note="color: #b7e6d7"
     misc_feature    9176..9367
                     /label=LCB3_v1.2
                     /note="color: #ff9ccd"
     misc_feature    9374..9523
                     /label=PDGFR-TMD
                     /note="color: #b7e6d7"
     misc_feature    9539..9592
                     /label=T2A
                     /note="color: #ff9ccd"
     misc_feature    9602..10309
                     /label=mCherry
                     /note="color: #f58a5e"
     misc_feature    10326..10917
                     /label=WPRE
                     /note="color: #dc143c"
     misc_feature    complement(10856..10859)
                     /label=Bbs1
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    complement(10862..10867)
                     /label=Bbs1
                     /note="color: #b1ff67; direction: LEFT"
ORIGIN
        1 cgtctcaagc atacacgata ccgtcgacca aggcagctgt agatcttagc cactttttaa
       61 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
      121 cttgtactgg gtttctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      181 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      241 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      301 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
      361 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
      421 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
      481 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
      541 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
      601 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      661 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      721 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
      781 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
      841 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag
      901 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      961 ccggcatccg cttacagaca agctgtgacc atctccggga gctgcatgtg tcagaggttt
     1021 tcaccgtcat caccgaaacg cgcgagatga aagggcctcg tgatacgcct atttttatag
     1081 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1141 ccgagcggcc gcacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1681 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgacagg
     1921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2761 tgggtcccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3001 tcgcggccgc caatttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     3241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3541 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     3601 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     3661 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     3721 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     3781 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     3841 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     3901 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     3961 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     4021 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     4081 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     4141 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     4201 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     4261 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     4321 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     4381 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     4441 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     4501 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     4561 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     4621 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     4681 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     4741 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     4801 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     4861 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     4921 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     4981 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     5041 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     5101 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     5161 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     5221 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5281 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5341 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5461 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     5521 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
     5581 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     5641 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     5701 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     5761 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     5821 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     5881 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     5941 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     6001 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     6061 ctcagatcct gcatataagc agctgctttt tgcctgtact gggactctct ggttagacca
     6121 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6181 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6241 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6301 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     6361 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     6421 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     6481 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     6541 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     6601 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     6661 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     6721 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     6781 gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga
     6841 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6901 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6961 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7021 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7081 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7141 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7201 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7261 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7321 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7381 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7441 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7501 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7561 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7621 gacagagaca gatccattcg attagtgaac ggatctcgac ggtcgccaaa tggcagtatt
     7681 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     7741 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     7801 aaattttcgg gtttattaca gggacagcag agatccagtt tggccctctg atgagacgaa
     7861 cgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     7921 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     7981 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     8041 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     8101 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     8161 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     8221 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     8281 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     8341 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     8401 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     8461 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     8521 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     8581 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     8641 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     8701 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     8761 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     8821 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     8881 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     8941 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     9001 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcca ctatggcctt
     9061 gcccgttacg gcacttctgc tcccactcgc attgctgctt cacgccgccc gaccagaaca
     9121 gaaactcata agtgaagaag accttggggg aggcggttcc gggggaggcg gttctaacga
     9181 tgacgagctc catatgcaga tgaccgatct ggtgtacgag gcactacact ttgctaaaga
     9241 cgaggaaatc cagaagcacg tcttccagct gttcgagaag gccacaaagg cctataaaaa
     9301 taaagacagg caaaaactgg aaaaggttgt ggaggaactg aaggaattgt tagagagact
     9361 tctcagcggt tctaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc
     9421 cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat
     9481 ctcccttatc atcctcatca tgctttggca gaagaagcca cgtggatccg ggagtggcga
     9541 aggcaggggc tcccttttga cgtgcgggga tgttgaagag aaccctgggc ccggatctgg
     9601 aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     9661 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     9721 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     9781 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     9841 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     9901 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     9961 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
    10021 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
    10081 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
    10141 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
    10201 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
    10261 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aatggcgctt
    10321 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10501 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10561 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10621 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10681 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10741 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10801 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10861 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatgct
    10921 g
//
