LOCUS       pHrD-IRES-Fluc          6135 bp    DNA     circular SYN 05-APR-2021
DEFINITION  Cloning vector pGL3-Basic, complete sequence.
ACCESSION   U47295 (modified)
VERSION     U47295.2
KEYWORDS    .
SOURCE      Cloning vector pGL3-Basic
  ORGANISM  Cloning vector pGL3-Basic
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Groskreutz,D.J. and Schenborn,E.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1996) D.J. Groskreutz, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Kenefick,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Technical Writing, Promega Corporation,
            2800 Woods Hollow Road, Madison, WI 53711-5399, USA
  REMARK    Sequence update by submitter
COMMENT     On Mar 5, 2001 this sequence version replaced U47295.1.
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Cloning vector pGL3-Basic"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45857"
                     /note="luciferase reporter vector"
     misc_feature    1..6
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    7..12
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    16..21
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     misc_feature    22..27
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    28..33
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    42..47
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     promoter        62..471
                     /created_by="User"
                     /modified_by="User"
                     /label="HrDNA_promoter (-410 to TSS)"
     source          <62..>798
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     primer_bind     62..84
                     /Sequence="CGCGATCCTTTCTGGAGAGTCCC"
                     /Hairpin_Tm="49.8"
                     /Tm="65.8"
                     /Self_Dimer_Tm="None"
                     /%GC="60.9"
                     /Extension="GTGGTACCC"
                     /modified_by="User"
                     /label="HrD_KpnI_F"
     misc_feature    424
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Spacer          472..798
                     /created_by="User"
                     /modified_by="User"
                     /label="pre-rRNA 5'ETS (+1 to +327)"
     primer_bind     534..551
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="4743815923423900c8c82107-57d0-4925-b11c
                     -56172488fcc2"
                     /%GC="66.7"
                     /Tm="61.1"
                     /Hairpin_Tm="43.6"
                     /Self_Dimer_Tm="11.7"
                     /Sequence="GCTCTGGCCTACCGGTGA"
                     /label="HrD_seqInt"
     misc_feature    complement(777..798)
                     /Sequence="GACGAGAACGCCTGACACGCAC"
                     /Hairpin_Tm="44.0"
                     /Tm="67.2"
                     /Self_Dimer_Tm="None"
                     /%GC="63.6"
                     /created_by="primer3"
                     /Extension="GGAGATCT"
                     /modified_by="User"
                     /label="HrD_Bgl2_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    799..804
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     Enhancer        893..>1391
                     /created_by="User"
                     /modified_by="User"
                     /label="IRES (CITE)"
     misc_feature    1042..1047
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    1259..1264
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    1390..1395
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    1403..1408
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     CDS             1405..3057
                     /gene="luc+"
                     /codon_start=1
                     /product="luciferase"
                     /protein_id="AAA89082.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            1405..3057
                     /gene="luc+"
     primer_bind     complement(1406..1428)
                     /note="GL primer2 sequencing primer binding site"
                     /Sequence="CTTTATGTTTTTGGCGTCTTCCA"
                     /Hairpin_Tm="None"
                     /Tm="58.0"
                     /Self_Dimer_Tm="None"
                     /%GC="39.1"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pGL3_seqR"
     misc_feature    2064..2069
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    3059..3064
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     regulatory      3089..3310
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     primer_bind     3163..3182
                     /created_by="primer3"
                     /annotation_group="58594628542739001efe2885-6483-4256-ba6a
                     -adf941c615db"
                     /%GC="35.0"
                     /Tm="51.1"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /Transferred_From="SV40pA-R"
                     /Transferred_Similarity="100.00%"
                     /label="SV40pA-R"
     misc_feature    3217..3222
                     /Recognition_pattern="GTT^AAC"
                     /annotation_group="GTTAAC"
                     /label="HpaI"
                     /note="Geneious type: restriction site"
     primer_bind     3253..3269
                     /created_by="primer3"
                     /annotation_group="5859608051861300c95a3de9-963d-4a21-bdc6
                     -86f5e136ebab"
                     /%GC="64.7"
                     /Tm="57.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTCAGGGGGAGGTGTG"
                     /Transferred_From="EGFP-C-Rev"
                     /Transferred_Similarity="100.00%"
                     /label="EGFP-C-Rev"
     misc_feature    3321..3326
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    3327..3332
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    3327..3332
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(3378..3397)
                     /note="RV primer4 sequencing primer binding site"
     primer_bind     complement(3519..3538)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="VR"
     misc_feature    complement(3579..4252)
                     /X-Transferred_From="pCRII-TOPO; pRL empty vector;
                     pRL-CMV"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pRL empty vector, pRL-CMV, pRL-SV40"
                     /Transferred_Similarity="100.00%"
                     /label="pUC origin"
                     /note="Geneious type: origin of replication"
     misc_feature    complement(3625..3644)
                     /modified_by="User"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="5290044573435009a1f04e4-6a93-4901-aa41-
                     38ca3d98ecce"
                     /%GC="50.0"
                     /Tm="60.3"
                     /Hairpin_Tm="39.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGGAAAAACGCCAGCAACG"
                     /label="pUC_ori_sqF"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      3635
                     /note="ColE1-derived plasmid replication origin"
     misc_feature    3891..3896
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     CDS             complement(4397..5257)
                     /gene="AmpR"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="AAA89083.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(4397..5257)
                     /gene="AmpR"
     misc_feature    4584..4594
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    4837..4842
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    5137..5142
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     primer_bind     5237..5257
                     /created_by="primer3"
                     /annotation_group="5686154009162200c2d23fdc-053e-4e7d-9764
                     -036a2028c4ac"
                     /%GC="33.3"
                     /Tm="53.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACGGAAATGTTGAATACTCAT"
                     /Transferred_From="AmpR_5p_SqR"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_5p_SqR"
     promoter        complement(5299..5327)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     rep_origin      5389..5844
                     /note="f1 origin"
     misc_feature    5852..5862
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    5883..5888
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    5967..5974
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     regulatory      5975..6128
                     /regulatory_class="polyA_signal_sequence"
                     /note="upstream poly(A) signal"
                     /modified_by="User"
                     /label="Synthetic polyA signal/transcriptional pause site
                     (for background reduction)"
     primer_bind     6077..6096
                     /note="RV primer3 sequencing primer binding site"
                     /Sequence="CTAGCAAAATAGGCTGTCCC"
                     /Hairpin_Tm="42.6"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pGL3_seqF"
ORIGIN      
        1 ggtaccgagc tctcccatat ggtcgacctg caggcggccg cgaattcact agtgattacc
       61 ccgcgatcct ttctggagag tccccgtgcg gagtcggaga gcgctccctg agcgcgcgtg
      121 cggcccgaga ggtcgcgcct ggccggcctt cggtccctcg tgtgtcccgg tcgtaggagg
      181 ggccggccga aaatgcttcc ggctcccgct ctggagacac gggccggccc cctgcgtgtg
      241 gcacgggcgg ccgggagggc gtccccggcc cggcgctgct cccgcgtgtg tcctggggtt
      301 gaccagaggg ccccgggcgc tccgtgtgtg gctgcgatgg tggcgttttt ggggacaggt
      361 gtccgtgtcg cgcgtcgcct gggccggcgg cgtggtcggt gacgcgacct cccggccccg
      421 ggggaggtat atctttcgct ccgagtcggc attttgggcc gccgggttat tgctgacacg
      481 ctgtcctctg gcgacctgtc gctggagagg ttgggcctcc ggatgcgcgc ggggctctgg
      541 cctaccggtg acccggctag ccggccgcgc tcctgcttga gccgcctgcc ggggcccgcg
      601 ggcctgctgt tctctcgcgc gtccgagcgt cccgactccc ggtgccggcc cgggtccggg
      661 tctctgaccc acccgggggc ggcggggaag gcggcgaggg ccaccgtgcc cccgtgcgct
      721 ctccgctgcg ggcgcccggg gcggccgcga caaccccacc ccgctggctc cgtgccgtgc
      781 gtgtcaggcg ttctcgtcag atctagggcg gccaattcgc ccctctcccc ccccccccta
      841 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
      901 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
      961 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     1021 tgaaggaagc agtacctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     1081 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     1141 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     1201 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     1261 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     1321 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     1381 cacgatgata agcttgccac aaccatggaa gacgccaaaa acataaagaa aggcccggcg
     1441 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     1501 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     1561 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     1621 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     1681 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     1741 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     1801 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     1861 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     1921 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     1981 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     2041 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     2101 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     2161 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     2221 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     2281 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     2341 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     2401 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     2461 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     2521 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     2581 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     2641 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     2701 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     2761 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     2821 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     2881 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     2941 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     3001 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc
     3061 tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     3121 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     3181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     3241 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     3301 aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt
     3361 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     3421 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     3481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     5401 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5461 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5521 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5581 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5641 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5701 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5761 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5821 acaaaatatt aacgcttaca atttgccatt cgccattcag gctgcgcaac tgttgggaag
     5881 ggcgatcggt gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta
     5941 atattaaggt acgggaggta cttggagcgg ccgcaataaa atatctttat tttcattaca
     6001 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     6061 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     6121 atttctctat cgata
//
