LOCUS       Exported               13728 bp DNA     circular SYN 22-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Nov 9, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13728
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2633..2715
                     /label=SagRNA scaffold
     misc_feature    2732..2976
                     /label=hU6
     misc_feature    3010..4212
                     
     CDS             4250..4276
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4277..7531
                     /label=dSaCas9
     CDS             4283..4303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7484..7531
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    7537..7844
                     /label=KRAB
     primer_bind     7818..7875
                     /label=SM185
     CDS             7826..7846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7853..7906
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7907..8395
                     /codon_start=1
                     /label=Thy1.1
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     primer_bind     complement(8372..8430)
                     /label=SM186
     primer_bind     complement(8373..8397)
                     /label=MluI-Thy1-REV
                     /note="Add MluI"
     misc_feature    8420..9008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8891..8902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9011..9027)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9533..9713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9745..9969
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10015..10443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10457..10786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10637..10772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10834..10881
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10900..11274
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11404..11525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11574..11590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11598..11614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11622..11652)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11667..11688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11976..12564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12735..13595)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13596..13700)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatga acaaaagctg gagctccacc gcgggcggcc gcaaaaaaat
     2641 ctcgccaaca agttgacgag ataaacacgg cattttgcct tgttttagta gattctgttt
     2701 ccagagtact aaaacagaga cgtccgtctc cggtgtttcg tcctttccac aagatatata
     2761 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     2821 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     2881 ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca gccttgtatc
     2941 gtatatgcaa atatgaagga atcatgggaa ataggcatta acccgtgtcg gctccagatc
     3001 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     3061 gccacgtcag acgaagggcg caggagcgtt cctgatcctt ccgcccggac gctcaggaca
     3121 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     3181 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     3241 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     3301 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     3361 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagttgcgg gctgctgggc
     3421 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
     3481 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     3541 gcacaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtaagg cgggctgtga
     3601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3721 gacgtgaagt ttgtcactga ctggagaact cgggtttgtc gtctggttgc gggggcggca
     3781 gttatgcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc tcgtcgtgtc
     3841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     4021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     4081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4201 tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatgt acccatacga
     4261 tgttccagat tacgctatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
     4321 agcagccaag cggaactaca tcctgggcct ggccatcggc atcaccagcg tgggctacgg
     4381 catcatcgac tacgagacac gggacgtgat cgatgccggc gtgcggctgt tcaaagaggc
     4441 caacgtggaa aacaacgagg gcaggcggag caagagaggc gccagaaggc tgaagcggcg
     4501 gaggcggcat agaatccaga gagtgaagaa gctgctgttc gactacaacc tgctgaccga
     4561 ccacagcgag ctgagcggca tcaaccccta cgaggccaga gtgaagggcc tgagccagaa
     4621 gctgagcgag gaagagttct ctgccgccct gctgcacctg gccaagagaa gaggcgtgca
     4681 caacgtgaac gaggtggaag aggacaccgg caacgagctg tccaccaaag agcagatcag
     4741 ccggaacagc aaggccctgg aagagaaata cgtggccgaa ctgcagctgg aacggctgaa
     4801 gaaagacggc gaagtgcggg gcagcatcaa cagattcaag accagcgact acgtgaaaga
     4861 agccaaacag ctgctgaagg tgcagaaggc ctaccaccag ctggaccaga gcttcatcga
     4921 cacctacatc gacctgctgg aaacccggcg gacctactat gagggacctg gcgagggcag
     4981 ccccttcggc tggaaggaca tcaaagaatg gtacgagatg ctgatgggcc actgcaccta
     5041 cttccccgag gaactgcgga gcgtgaagta cgcctacaac gccgacctgt acaacgccct
     5101 gaacgacctg aacaatctcg tgatcaccag ggacgagaac gagaagctgg aatattacga
     5161 gaagttccag atcatcgaga acgtgttcaa gcagaagaag aagcccaccc tgaagcagat
     5221 cgccaaagaa atcctcgtga acgaagagga tattaagggc tacagagtga ccagcaccgg
     5281 caagcccgag ttcaccaacc tgaaggtgta ccacgacatc aaggacatta ccgcccggaa
     5341 agagattatt gagaacgccg agctgctgga tcagattgcc aagatcctga ccatctacca
     5401 gagcagcgag gacatccagg aagaactgac caatctgaac tccgagctga cccaggaaga
     5461 gatcgagcag atctctaatc tgaagggcta taccggcacc cacaacctga gcctgaaggc
     5521 catcaacctg atcctggacg agctgtggca caccaacgac aaccagatcg ctatcttcaa
     5581 ccggctgaag ctggtgccca agaaggtgga cctgtcccag cagaaagaga tccccaccac
     5641 cctggtggac gacttcatcc tgagccccgt cgtgaagaga agcttcatcc agagcatcaa
     5701 agtgatcaac gccatcatca agaagtacgg cctgcccaac gacatcatta tcgagctggc
     5761 ccgcgagaag aactccaagg acgcccagaa aatgatcaac gagatgcaga agcggaaccg
     5821 gcagaccaac gagcggatcg aggaaatcat ccggaccacc ggcaaagaga acgccaagta
     5881 cctgatcgag aagatcaagc tgcacgacat gcaggaaggc aagtgcctgt acagcctgga
     5941 agccatccct ctggaagatc tgctgaacaa ccccttcaac tatgaggtgg accacatcat
     6001 ccccagaagc gtgtccttcg acaacagctt caacaacaag gtgctcgtga agcaggaaga
     6061 agccagcaag aagggcaacc ggaccccatt ccagtacctg agcagcagcg acagcaagat
     6121 cagctacgaa accttcaaga agcacatcct gaatctggcc aagggcaagg gcagaatcag
     6181 caagaccaag aaagagtatc tgctggaaga acgggacatc aacaggttct ccgtgcagaa
     6241 agacttcatc aaccggaacc tggtggatac cagatacgcc accagaggcc tgatgaacct
     6301 gctgcggagc tacttcagag tgaacaacct ggacgtgaaa gtgaagtcca tcaatggcgg
     6361 cttcaccagc tttctgcggc ggaagtggaa gtttaagaaa gagcggaaca aggggtacaa
     6421 gcaccacgcc gaggacgccc tgatcattgc caacgccgat ttcatcttca aagagtggaa
     6481 gaaactggac aaggccaaaa aagtgatgga aaaccagatg ttcgaggaaa agcaggccga
     6541 gagcatgccc gagatcgaaa ccgagcagga gtacaaagag atcttcatca ccccccacca
     6601 gatcaagcac attaaggact tcaaggacta caagtacagc caccgggtgg acaagaagcc
     6661 taatagagag ctgattaacg acaccctgta ctccacccgg aaggacgaca agggcaacac
     6721 cctgatcgtg aacaatctga acggcctgta cgacaaggac aatgacaagc tgaaaaagct
     6781 gatcaacaag agccccgaaa agctgctgat gtaccaccac gacccccaga cctaccagaa
     6841 actgaagctg attatggaac agtacggcga cgagaagaat cccctgtaca agtactacga
     6901 ggaaaccggg aactacctga ccaagtactc caaaaaggac aacggccccg tgatcaagaa
     6961 gattaagtat tacggcaaca aactgaacgc ccatctggac atcaccgacg actaccccaa
     7021 cagcagaaac aaggtcgtga agctgtccct gaagccctac agattcgacg tgtacctgga
     7081 caatggcgtg tacaagttcg tgaccgtgaa gaatctggat gtgatcaaaa aagaaaacta
     7141 ctacgaagtg aatagcaagt gctatgagga agctaagaag ctgaagaaga tcagcaacca
     7201 ggccgagttt atcgcctcct tctacaacaa cgatctgatc aagatcaacg gcgagctgta
     7261 tagagtgatc ggcgtgaaca acgacctgct gaaccggatc gaagtgaaca tgatcgacat
     7321 cacctaccgc gagtacctgg aaaacatgaa cgacaagagg ccccccagga tcattaagac
     7381 aatcgcctcc aagacccaga gcattaagaa gtacagcaca gacattctgg gcaacctgta
     7441 tgaagtgaaa tctaagaagc accctcagat catcaaaaag ggcaaaaggc cggcggccac
     7501 gaaaaaggcc ggccaggcaa aaaagaaaaa gggatccgat gctaagtcac tgactgcctg
     7561 gtcccggaca ctggtgacct tcaaggatgt gtttgtggac ttcaccaggg aggagtggaa
     7621 gctgctggac actgctcagc agatcctgta cagaaatgtg atgctggaga actataagaa
     7681 cctggtttcc ttgggttatc agcttactaa gccagatgtg atcctccggt tggagaaggg
     7741 agaagagccc tggctggtgg agagagaaat tcaccaagag acccatcctg attcagagac
     7801 tgcatttgaa atcaaatcat cagttccgaa aaagaaacgc aaagttgcta gcgagggcag
     7861 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga acccagccat
     7921 cagcgtcgct ctcctgctct cagtcttgca ggtgtcccga gggcagaagg tgaccagcct
     7981 gacagcctgc ctggtgaacc aaaaccttcg cctggactgc cgccatgaga ataacaccaa
     8041 ggataactcc atccagcatg agttcagcct gacccgagag aagaggaagc acgtgctctc
     8101 aggcaccctt gggatacccg agcacacgta ccgctcccgc gtcaccctct ccaaccagcc
     8161 ctatatcaag gtccttaccc tagccaactt caccaccaag gatgagggcg actacttttg
     8221 tgagcttcgc gtttcgggcg cgaatcccat gagctccaat aaaagtatca gtgtgtatag
     8281 agacaagctg gtcaagtgtg gcggcataag cctgctggtt cagaacacat cctggatgct
     8341 gctgctgctg ctttccctct ccctcctcca agccctggac ttcatttctc tgtgagaatt
     8401 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8461 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8521 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8581 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8641 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8701 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8761 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8821 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8881 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8941 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9001 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     9061 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     9121 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     9181 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9241 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     9301 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     9361 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     9421 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     9481 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     9541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     9721 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     9781 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     9841 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     9901 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     9961 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
    10021 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    10081 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10141 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10201 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10261 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10321 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    10381 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    10441 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
    10501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
    10561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
    10621 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10681 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
    10741 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
    10801 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
    10861 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
    10921 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
    10981 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
    11041 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
    11101 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
    11161 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
    11221 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
    11281 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    11341 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    11401 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11521 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    11581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    11641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    11701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    11761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    11821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    11881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    11941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    12061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    12121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    12181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    12241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    12301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    12421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    12481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    13021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    13081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    13141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    13201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    13261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    13321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    13381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    13441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    13501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
