LOCUS       Exported               13812 bp DNA     circular SYN 22-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Nov 9, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13812
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        29..133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             134..994
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2041..2062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2077..2107
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2115..2131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2204..2325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2455..2829)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     2605..2622
                     /label=SM68_eGFP_gibson_REV
     promoter        complement(2848..2895)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2943..3272)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2957..3092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3286..3714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    3760..3984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             4016..4196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4702..4718
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4721..5309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4827..4838
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5117..5136
                     /label=WPRE_REV
     primer_bind     5299..5357
                     /label=SM186
     primer_bind     5332..5356
                     /label=MluI-Thy1-REV
                     /note="Add MluI"
     CDS             complement(5334..5822)
                     /codon_start=1
                     /label=Thy1.1
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             complement(5823..5876)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     5848..5905
                     /label=SM98_VP64_gibson_REV
     primer_bind     5848..5905
                     /label=Fragment 2.REV
     primer_bind     complement(5854..5904)
                     /label=SM185
     primer_bind     complement(5863..5904)
                     /label=SM69_T2A_eGFP_gibson_FW
     CDS             complement(5883..5903)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5904..5918)
                     /label=Fragment 2.FOR
     CDS             complement(5928..6077)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(6090..6136)
                     /label=SM96_NEW_VP64_gibson_FW
     CDS             complement(6108..9362)
                     /codon_start=1
                     /label=dSaCas9
                     /translation="MAPKKKRKVGIHGVPAAKRNYILGLAIGITSVGYGIIDYETRDVI
                     DAGVRLFKEANVENNEGRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINP
                     YEARVKGLSQKLSEEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEE
                     KYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLE
                     TRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNL
                     VITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFT
                     NLKVYHDIKDITARKEIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQI
                     SNLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVD
                     DFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQT
                     NERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIP
                     RSVSFDNSFNNKVLVKQEEASKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRIS
                     KTKKEYLLEERDINRFSVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSING
                     GFTSFLRRKWKFKKERNKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQ
                     AESMPEIETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDK
                     GNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLY
                     KYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRF
                     DVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIK
                     INGELYRVIGVNNDLLNRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYST
                     DILGNLYEVKSKKHPQIIKKGKRPAATKKAGQAKKKK"
     CDS             complement(6108..6155)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(9336..9356)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9343..9374)
                     /label=Fragment 2.FOR
     primer_bind     complement(9343..9362)
                     /label=pSM133
     primer_bind     complement(9343..9362)
                     /label=Fragment 2.FOR
     regulatory      9359..9362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9387..9536)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    9516
                     /label=Synonymous Mutation (T->C)
                     /note="Reduces degree of overlap between other VP64 
                     sequence"
     misc_feature    9519
                     /label=Synonymous Mutation (C->T)
                     /note="Required for gblock synthesis"
     CDS             complement(9537..9563)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9551..9607)
                     /label=SM130_VP64_Linker_REV
     primer_bind     complement(9588..9607)
                     /label=SM164_VP64_dSaCas9_gblock1_FW
     misc_feature    9601..10803
                     
     primer_bind     10738..10757
                     /label=SM16_UbC_Rev
     misc_feature    10837..11081
                     /label=hU6
     misc_feature    11098..11180
                     /label=SagRNA scaffold
     misc_feature    11256..11373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11900..12133
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12626..12751
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12798..12978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(12996..13194)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        13196..13575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1981 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2161 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     2221 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2281 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     2341 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     2401 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc
     2461 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     2521 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     2581 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     2641 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     2701 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     2761 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     2821 cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga
     2881 tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc
     2941 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     3001 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     3061 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     3121 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3181 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3241 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     3301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     3721 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     3781 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     3841 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     3901 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     3961 atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta
     4021 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     4081 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4141 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4201 cagtccggat gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac
     4261 tctaatactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg
     4321 gtgttctctc cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag
     4381 caccatccaa aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca
     4441 gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag
     4501 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     4561 ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc
     4621 tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt
     4681 gctccatgtt tttctaggtc tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg
     4741 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     4801 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     4861 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     4921 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     4981 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     5041 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     5101 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     5161 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     5221 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     5281 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa ttctcacaga
     5341 gaaatgaagt ccagggcttg gaggagggag agggaaagca gcagcagcag catccaggat
     5401 gtgttctgaa ccagcaggct tatgccgcca cacttgacca gcttgtctct atacacactg
     5461 atacttttat tggagctcat gggattcgcg cccgaaacgc gaagctcaca aaagtagtcg
     5521 ccctcatcct tggtggtgaa gttggctagg gtaaggacct tgatataggg ctggttggag
     5581 agggtgacgc gggagcggta cgtgtgctcg ggtatcccaa gggtgcctga gagcacgtgc
     5641 ttcctcttct ctcgggtcag gctgaactca tgctggatgg agttatcctt ggtgttattc
     5701 tcatggcggc agtccaggcg aaggttttgg ttcaccaggc aggctgtcag gctggtcacc
     5761 ttctgccctc gggacacctg caagactgag agcaggagag cgacgctgat ggctgggttc
     5821 atagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct gccctcgcta
     5881 gcaactttgc gtttcttttt cggagatccg gaacttctag agttaatcag catgtccagg
     5941 tcgaaatcat caagggcgtc actgccgagc atgtcgaggt caaagtcatc aagggcatcc
     6001 gaaccaagca tgtcaaggtc aaaatcatcg agggcgtcac ttcccagcat atccagatca
     6061 aaatcgtcca atgcgtcagc ccgtccggaa ccgctggcct cggatccctt tttctttttt
     6121 gcctggccgg cctttttcgt ggccgccggc cttttgccct ttttgatgat ctgagggtgc
     6181 ttcttagatt tcacttcata caggttgccc agaatgtctg tgctgtactt cttaatgctc
     6241 tgggtcttgg aggcgattgt cttaatgatc ctggggggcc tcttgtcgtt catgttttcc
     6301 aggtactcgc ggtaggtgat gtcgatcatg ttcacttcga tccggttcag caggtcgttg
     6361 ttcacgccga tcactctata cagctcgccg ttgatcttga tcagatcgtt gttgtagaag
     6421 gaggcgataa actcggcctg gttgctgatc ttcttcagct tcttagcttc ctcatagcac
     6481 ttgctattca cttcgtagta gttttctttt ttgatcacat ccagattctt cacggtcacg
     6541 aacttgtaca cgccattgtc caggtacacg tcgaatctgt agggcttcag ggacagcttc
     6601 acgaccttgt ttctgctgtt ggggtagtcg tcggtgatgt ccagatgggc gttcagtttg
     6661 ttgccgtaat acttaatctt cttgatcacg gggccgttgt cctttttgga gtacttggtc
     6721 aggtagttcc cggtttcctc gtagtacttg tacaggggat tcttctcgtc gccgtactgt
     6781 tccataatca gcttcagttt ctggtaggtc tgggggtcgt ggtggtacat cagcagcttt
     6841 tcggggctct tgttgatcag ctttttcagc ttgtcattgt ccttgtcgta caggccgttc
     6901 agattgttca cgatcagggt gttgcccttg tcgtccttcc gggtggagta cagggtgtcg
     6961 ttaatcagct ctctattagg cttcttgtcc acccggtggc tgtacttgta gtccttgaag
     7021 tccttaatgt gcttgatctg gtggggggtg atgaagatct ctttgtactc ctgctcggtt
     7081 tcgatctcgg gcatgctctc ggcctgcttt tcctcgaaca tctggttttc catcactttt
     7141 ttggccttgt ccagtttctt ccactctttg aagatgaaat cggcgttggc aatgatcagg
     7201 gcgtcctcgg cgtggtgctt gtaccccttg ttccgctctt tcttaaactt ccacttccgc
     7261 cgcagaaagc tggtgaagcc gccattgatg gacttcactt tcacgtccag gttgttcact
     7321 ctgaagtagc tccgcagcag gttcatcagg cctctggtgg cgtatctggt atccaccagg
     7381 ttccggttga tgaagtcttt ctgcacggag aacctgttga tgtcccgttc ttccagcaga
     7441 tactctttct tggtcttgct gattctgccc ttgcccttgg ccagattcag gatgtgcttc
     7501 ttgaaggttt cgtagctgat cttgctgtcg ctgctgctca ggtactggaa tggggtccgg
     7561 ttgcccttct tgctggcttc ttcctgcttc acgagcacct tgttgttgaa gctgttgtcg
     7621 aaggacacgc ttctggggat gatgtggtcc acctcatagt tgaaggggtt gttcagcaga
     7681 tcttccagag ggatggcttc caggctgtac aggcacttgc cttcctgcat gtcgtgcagc
     7741 ttgatcttct cgatcaggta cttggcgttc tctttgccgg tggtccggat gatttcctcg
     7801 atccgctcgt tggtctgccg gttccgcttc tgcatctcgt tgatcatttt ctgggcgtcc
     7861 ttggagttct tctcgcgggc cagctcgata atgatgtcgt tgggcaggcc gtacttcttg
     7921 atgatggcgt tgatcacttt gatgctctgg atgaagcttc tcttcacgac ggggctcagg
     7981 atgaagtcgt ccaccagggt ggtggggatc tctttctgct gggacaggtc caccttcttg
     8041 ggcaccagct tcagccggtt gaagatagcg atctggttgt cgttggtgtg ccacagctcg
     8101 tccaggatca ggttgatggc cttcaggctc aggttgtggg tgccggtata gcccttcaga
     8161 ttagagatct gctcgatctc ttcctgggtc agctcggagt tcagattggt cagttcttcc
     8221 tggatgtcct cgctgctctg gtagatggtc aggatcttgg caatctgatc cagcagctcg
     8281 gcgttctcaa taatctcttt ccgggcggta atgtccttga tgtcgtggta caccttcagg
     8341 ttggtgaact cgggcttgcc ggtgctggtc actctgtagc ccttaatatc ctcttcgttc
     8401 acgaggattt ctttggcgat ctgcttcagg gtgggcttct tcttctgctt gaacacgttc
     8461 tcgatgatct ggaacttctc gtaatattcc agcttctcgt tctcgtccct ggtgatcacg
     8521 agattgttca ggtcgttcag ggcgttgtac aggtcggcgt tgtaggcgta cttcacgctc
     8581 cgcagttcct cggggaagta ggtgcagtgg cccatcagca tctcgtacca ttctttgatg
     8641 tccttccagc cgaaggggct gccctcgcca ggtccctcat agtaggtccg ccgggtttcc
     8701 agcaggtcga tgtaggtgtc gatgaagctc tggtccagct ggtggtaggc cttctgcacc
     8761 ttcagcagct gtttggcttc tttcacgtag tcgctggtct tgaatctgtt gatgctgccc
     8821 cgcacttcgc cgtctttctt cagccgttcc agctgcagtt cggccacgta tttctcttcc
     8881 agggccttgc tgttccggct gatctgctct ttggtggaca gctcgttgcc ggtgtcctct
     8941 tccacctcgt tcacgttgtg cacgcctctt ctcttggcca ggtgcagcag ggcggcagag
     9001 aactcttcct cgctcagctt ctggctcagg cccttcactc tggcctcgta ggggttgatg
     9061 ccgctcagct cgctgtggtc ggtcagcagg ttgtagtcga acagcagctt cttcactctc
     9121 tggattctat gccgcctccg ccgcttcagc cttctggcgc ctctcttgct ccgcctgccc
     9181 tcgttgtttt ccacgttggc ctctttgaac agccgcacgc cggcatcgat cacgtcccgt
     9241 gtctcgtagt cgatgatgcc gtagcccacg ctggtgatgc cgatggccag gcccaggatg
     9301 tagttccgct tggctgctgg gactccgtgg ataccgacct tccgcttctt ctttggggcc
     9361 atagagccgg aactcctaga gttaattaac atatcgagat cgaaatcgtc cagagcatcg
     9421 gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca
     9481 aagtcatcga gggcatcaga acccagcatg tcgaggtcaa aatcgtccag cgcgtcagcg
     9541 taatctggaa catcgtatgg gtacatggtg gctctagagt cgacctgcag cccaagcttc
     9601 gtctaacaaa aaagccaaaa acggccagaa tttagcggac aatttactag tctaacactg
     9661 aaaattacat attgacccaa atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa
     9721 cacactcgcc aaccccgagc gcatagttca aaaccggagc ttcagctact taagaagata
     9781 ggtacataaa accgaccaaa gaaactgacg cctcacttat ccctcccctc accagaggtc
     9841 cggcgcctgt cgattcagga gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag
     9901 aaaagcctac cgcacaccta ccggcaggtg gccccaccct gcattataag ccaacagaac
     9961 gggtgacgtc acgacacgac gaggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg
    10021 gcaccgcata actgccgccc ccgcaaccag acgacaaacc cgagttctcc agtcagtgac
    10081 aaacttcacg tcagggtccc cagatggtgc cccagcccat ctcacccgaa taagagcttt
    10141 cccgcattag cgaaggcctc aagaccttgg gttcttgccg cccaccatgc cccccacctt
    10201 gtttcaacga cctcacagcc cgccttacaa gcgtcttcca ttcaagactc gggaacagcc
    10261 gccattttgt gcgctccccc caacccccag ttcagggcaa ccttgctcgc ggacccagac
    10321 tacagccctt ggcggtctct ccacacgctt ccgtcccacc gagcggcccg gcggccacga
    10381 aagccccggc cagcccagca gcccgcaact caccaagtga cgatcacagc gatccacaaa
    10441 caagaaccgc gacccaaatc ccggctgcga cggaactagc tgtgccacac ccggcgcgtc
    10501 cttatataat catcggcgtt caccgcccca cggagatccc tccgcagaat cgccgagaag
    10561 ggactacttt tcctcgcctg ttccgctctc tggaaagaaa accagtgccc tagagtcacc
    10621 caagtcccgt cctaaaatgt ccttctgctg atactggggt tctaaggccg agtcttatga
    10681 gcagcgggcc gctgtcctga gcgtccgggc ggaaggatca ggaacgctcc tgcgcccttc
    10741 gtctgacgtg gcagcgctcg ccgtgaggag gggggcgccc gcgggaggcg ccaaaacccg
    10801 gcgcggaggc cagatctgga gccgacacgg gttaatgcct atttcccatg attccttcat
    10861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
    10921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
    10981 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
    11041 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacgga cgtctctgtt
    11101 ttagtactct ggaaacagaa tctactaaaa caaggcaaaa tgccgtgttt atctcgtcaa
    11161 cttgttggcg agattttttt gcggccgccc gcggtggagc tccagctttt gttcattaac
    11221 caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
    11281 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
    11341 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctg
    11401 cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt ctctgtctct
    11461 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
    11521 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
    11581 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
    11641 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
    11701 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
    11761 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
    11821 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
    11881 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
    11941 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
    12001 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
    12061 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
    12121 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
    12181 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
    12241 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg
    12301 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
    12361 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
    12421 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
    12481 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
    12541 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
    12601 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
    12661 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
    12721 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
    12781 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
    12841 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
    12901 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
    12961 gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc tcccaccgta
    13021 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
    13081 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
    13141 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
    13201 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
    13261 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
    13321 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
    13381 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
    13441 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
    13501 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
    13561 tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc
    13621 taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa
    13681 ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga tactggctta
    13741 actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg ggagatctcc
    13801 cgatccgtcg ac
//
